[1]刘振宇,王宏伟.小鼠原位肺癌模型构建及在放射治疗研究中的意义[J].陕西医学杂志,2022,51(7):791-794,810.[doi:DOI:10.3969/j.issn.1000-7377.2022.07.005]
　LIU Zhenyu,WANG Hongwei.Establishment of mouse model of lung cancer in situ and its significance in study of radiation therapy research[J].,2022,51(7):791-794,810.[doi:DOI:10.3969/j.issn.1000-7377.2022.07.005]
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小鼠原位肺癌模型构建及在放射治疗研究中的意义
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年7期
页码:
791-794,810
栏目:
基础研究
出版日期:
2022-07-05

文章信息/Info

Title:
Establishment of mouse model of lung cancer in situ and its significance in study of radiation therapy research
作者:
刘振宇; 王宏伟
(内蒙古医科大学附属医院放射治疗科,内蒙古 呼和浩特 010050)
Author(s):
LIU Zhenyu; WANG Hongwei
(Department of Radiotherapy,First Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010050,China)
关键词:
原位模型;  肺癌;  影像学;  放射治疗;  小鼠;  磁共振成像
Keywords:
In situ model;  Lung cancer;  Imaging;  Radiation therapy;  Mice;  MRI
分类号:
R 734.2
DOI:
DOI:10.3969/j.issn.1000-7377.2022.07.005
文献标志码:
A
摘要:
目的:构建小鼠原位肺癌模型,探讨其在放射治疗研究中的意义。方法:采用10只C57BL/6J小鼠,于左肺上叶接种鼠源性小鼠肺癌细胞,构建小鼠原位肺癌模型,观察小鼠接种肿瘤期间饮食、体重变化、病理学诊断及肺部磁共振成像(MRI)表现。结果:实验小鼠模型通过MRI及病理学验证,10只肺癌模型均构建成功,成功率为100%,成瘤时间为移植后7～14 d期间,21 d影像提示小鼠出现肺内、胸膜及淋巴结转移,在23 d统一处死后未发现肝脏及肾脏转移。结论:以鼠源性肺癌细胞构建的原位肺癌模型可重复,造模时间短,成功率高,肿瘤模型具有良好的稳定性和一致性; MRI验证可明显且直观体现肿瘤生长期,为后续开展放射治疗体内实验研究打下良好基础。
Abstract:
Objective:To establish mouse model of lung cancer in situ and to explore its significance in the study of radiation therapy research.Methods:Ten C57BL/6J mice were inoculated with mouse-derived lung cancer cells in the upper lobe of the left lung to establish a mouse model of lung cancer in situ.Diet,body weight changes,pathological diagnosis and lung magnetic resonance imaging(MRI)findings during tumor inoculation were observed.Results:The experimental mouse models were verified by MRI and pathology.All the 10 lung cancer models were successfully constructed with a success rate of 100%.The tumor formation time was 7 to 14 days after transplantation.On the 21st day,imaging suggested intrapulmonary,pleural,and lymph node metastasis in mice.On the 23st day,no liver and kidney metastasis was found after after sacrifice.Conclusion:The in situ lung cancer model constructed with mouse-derived lung cancer cells is reproducible,with short modeling time and high success rate.The tumor model has good stability and consistency.MRI verification can clearly and intuitively reflect the tumor growth stage.It lays a good foundation for the subsequent in vivo experimental research of radiation therapy.

参考文献/References:

[1] Altorki NK,Markowitz GJ,Gao D,et al.The lung microenvironment:An important regulator of tumour growth and metastasis[J].Nat Rev Cancer,2019,19(1):9-31.
[2] 郑伟珍,吴爱兵,杨志雄.移植性肺癌动物模型建立的研究进展[J].海南医学,2021,32(7):917-920.
[3] Day CP,Merlino G,Van Dyke T.Preclinical mouse cancer models:A maze of opportunities and challenges[J].Cell,2015,163(1):39-53.
[4] 周足力,陈修远,张鑫淼,等.肺癌人源性异种移植小鼠模型库的建立[J].中华实验外科杂志,2019,36(1):163-166.
[5] 胥孜杭,刘 菲,邹纯朴,等.小鼠肺癌原位模型的建立[J].中国医药导报,2015,12(1):15-18,169.
[6] 侯 敏,黄健清,戴丽军.化学致癌剂诱发的小鼠肺癌模型及应用研究[J].医学综述,2015,21(4):615-618.
[7] Spella M,Lilis I,Pepe MA,et al.Club cells form lung adenocarcinomas and maintain the alveoli of adult mice[J].Elife,2019,29(8):e45571.
[8] Ge GZ,Xu TR,Chen C.Tobacco carcinogen NNK-induced lung cancer animal models and associated carcinogenic mechanisms[J].Acta Biochim Biophys Sin(Shanghai),2015,47(7):477-487.
[9] Wang Y,Zhang Z,Yan Y,et al.A chemically induced model for squamous cell carcinoma of the lung in mice:Histopathology and strain susceptibility[J].Cancer Res,2004,64(5):1647-1654.
[10] Mervai Z,Egedi K,Kovalszky I,et al.Diethylnitrosamine induces lung adenocarcinoma in FVB/N mouse[J].BMC Cancer,2018,18(1):157.
[11] 吴逸明,李卫民,李卓炜,等.大鼠肺癌发生发展动物模型的构建[J].河南医科大学学报,2000,35(3):232-235,280.
[12] 鲁植艳.改良型大鼠肺鳞癌发展模型的建立[C]//中华医学会、中华医学会放射学分会.中华医学会第十三届全国放射学大会论文汇编(下册)[C].中华医学会放射学分会,2006:1.
[13] 马雪曼,王笑民,于明薇,等.小鼠lewis肺癌原位移植瘤和异位皮下移植瘤模型的对比研究[J].实用癌症杂志,2018,33(4):523-526.
[14] Bibby MC.Orthotopic models of cancer for preclinical drug evaluation:Advantages and disadvantages[J].Eur J Cancer,2004,40(6):852-857.
[15] 张贺龙,矢野圣二,三木丰和.经气管接种肺癌细胞建立肺癌动物模型的经验教训[J].第四军医大学学报,2002,23(17):1586.
[16] Ng SR,Rideout WM,Akama-Garren EH,et al.CRISPR-mediated modeling and functional validation of candidate tumor suppressor genes in small cell lung cancer[J].Proc Natl Acad Sci USA,2020,117(1):513-521.
[17] 佘佐亚,罗殿中,邝晓聪,等.绿色荧光蛋白转基因小鼠肺癌模型的建立[J].环境与健康杂志,2012,29(8):686-689,769.
[18] 张百红,岳红云.肿瘤微环境与肿瘤[J].国际肿瘤学杂志,2013,40(8):582-584.
[19] 贺春艳,吴月明.非小细胞肺癌患者缺氧诱导因子、血管内皮细胞生长因子表达水平及其临床意义[J].陕西医学杂志,2022,51(4):503-506.
[20] 岳 英,董锦华,肖月梅.肺免疫微环境中调节因子表达对肿瘤生长和转移的影响[J].陕西医学杂志,2021,50(4):495-498,502.

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备注/Memo

备注/Memo:
基金项目:内蒙古自治区自然科学基金资助项目(2019MS08024)
更新日期/Last Update: 2022-07-05