[1]韩海英,郭秀伟,张培彤.川芎对肺癌干细胞原发性肿瘤相关黏附分子影响的实验研究[J].陕西医学杂志,2024,(4):435-438,448.[doi:DOI:10.3969/j.issn.1000-7377.2024.04.001]
　HAN Haiying,GUO Xiuwei,ZHANG Peitong.Effect of Rhizoma Chuanxiong on primary tumor-associated adhesion molecules of lung cancer stem cells[J].,2024,(4):435-438,448.[doi:DOI:10.3969/j.issn.1000-7377.2024.04.001]
点击复制

川芎对肺癌干细胞原发性肿瘤相关黏附分子影响的实验研究
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年4期
页码:
435-438,448
栏目:
基础研究
出版日期:
2024-04-05

文章信息/Info

Title:
Effect of Rhizoma Chuanxiong on primary tumor-associated adhesion molecules of lung cancer stem cells
作者:
韩海英1; 郭秀伟2; 张培彤2
(1.首都医科大学附属北京友谊医院,北京 100050; 2.中国中医科学院广安门医院肿瘤科,北京 100053)
Author(s):
HAN Haiying; GUO Xiuwei; ZHANG Peitong
(Beijing Friendship Hospital Affiliated to Capital Medical University,Beijing 100050,China)
关键词:
川芎;  肺癌;  CD44v6;  β1整合素;  干细胞;  黏附
Keywords:
Rhizoma chuanxiong;  Lung cancer;  CD44v6;  β1-integrin;  Stem cells;  Adhesion
分类号:
R 73-3
DOI:
DOI:10.3969/j.issn.1000-7377.2024.04.001
文献标志码:
A
摘要:
目的:探究川芎对肺癌干细胞样细胞荷瘤小鼠原发肿瘤的黏附作用机制。方法:通过无血清培养技术,培养肺癌细胞中的干细胞样细胞,并将其种植在小鼠脚垫。按照处理药物分为空白对照组(Con组)、川芎组(RC组)、顺铂组(DDP组)、川芎+顺铂组(RC+DDP组)并干预,3周后,在无菌环境中手术剥取肿瘤组织。通过Western blot实验技术和免疫组化技术,检测分析小鼠肿瘤细胞中相关黏附蛋白CD44v6、β1整合素的表达。结果:CD44v6在RC+DDP中的表达较DDP组、RC组及Con组减少,且差异具有统计学意义(均P<0.05)。β1整合素原发瘤组织在RC+DDP组中的表达低于Con组、RC组及RC+DDP组(均P<0.05)。结论:川芎和顺铂的联合应用,在一定程度上可减少肺癌干细胞样细胞原发肿瘤中黏附分子CD44v6、β1整合素的表达,从而抑制其黏附能力。
Abstract:
Objective:To investigate the adhesion mechanism of Rhizoma Chuanxiong to primary tumor in lung cancer stem cell-like cells primary tumors in mouse model.Methods:Lung cancer stem-like cells were cultured by using serum-free culture techniques and implanted into mouse footpads.According to the treatment drugs,they were divided into blank control group(Con group),Rhizoma Chuanxiong group(RC group),cisplatin group(DDP group),Rhizoma Chuanxiong plus cisplatin group(RC+DDP group)and intervened,and the tumor tissues were surgically removed in a sterile environment after 3 weeks.The expression of adhesion proteins CD44v6 and β1-integrin in mouse tumor cells was analyzed by using Western blot and immunohistochemistry techniques.Results:The expression of CD44v6 in the RC+DDP group was significantly lower than that in the DDP,RC,and Con groups(all P<0.05).The expression of β1-integrin in the primary tumor tissues in the RC+DDP group was lower compared to the Con,RC,and RC+DDP groups(all P<0.05).Conclusion:The combination of Rhizoma Chuanxiong and cisplatin can inhibit the adhesion ability of lung cancer stem cell-like cells primary tumor by reducing the expression of adhesion molecules CD44v6 and β1-integrin.

参考文献/References:

[1] 郭家毓,呼群.小细胞肺癌免疫治疗研究进展[J].陕西医学杂志,2023,52(1):118-120.
[2] ZHANG Y,LUO G,ETXEBERRIA J,et al.Global patterns and trends in lung cancer incidence:A population-based study[J].J Thorac Oncol,2021,16(6):933-944.
[3] 王玲,申鸿.癌胚抗原、糖类抗原、CA125、血管生成素、细胞角蛋白19片段及甲胎蛋白检测在肺癌诊断中的临床研究[J].陕西医学杂志,2019,48(8):1080-1084.
[4] SUNG H,FERLAY J,SIEGEL R L,et al.Global cancer statistics 2020:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J].CA Cancer J Clin,2021,71(3):209-249.
[5] HE S,LI H,CAO M,et al.Trends and risk factors of lung cancer in China[J].Chin J Cancer Res,2020,32(6):683-694.
[6] 李健,贾瑞,王超,等.健脾益气汤联合化疗对肺癌术后患者免疫功能的影响[J].陕西中医,2023,44(4):459-462.
[7] TAO J,ZHU L,YAKOUB M,et al.Cell-Cell interactions drive metastasis of circulating tumor microemboli[J].Cancer Res,2022,82(15):2661-2671.
[8] 杨玲,李连弟,陈育德,等.中国肺癌死亡趋势分析及发病、死亡的估计与预测[J].中国肺癌杂志,2005,8(4):274-278.
[9] WANG W,JIN J,ZHOU Z,et al.Snail inhibits metastasis via regulation of Ecadherin and is associated with prognosis in colorectal cancer[J].Oncol Lett,2023,25(6):271.
[10] 朱鸿宇,陈鹏,董国璋,等.肺腺癌中免疫微环境特征与EGFR突变状态的相关性研究[J].中国肺癌杂志,2023,26(3):204-216.
[11] 刘宝瑞,徐修礼,刘文超,等.4种中药制剂对人肺癌A549细胞增殖的影响[J].中国药理学通报,2002,18(1):94-96.
[12] BARTOLOMÉR A,AIZPURUA C,JAÉN M,et al.Monoclonal antibodies directed against cadherin RGD exhibit therapeutic activity against melanoma and colorectal cancer metastasis[J].Clin Cancer Res,2018,24(2):433-444.
[13] ZHANG Q,ZHANG S,CHEN J,et al.The interplay between integrins and immune cells as a regulator in cancer immunology[J].Int J Mol Sci,2023,24(7):6170.
[14] ZHAO G,GONG L,SU D,et al.Cullin5 deficiency promotes small-cell lung cancer metastasis by stabilizing integrin β1[J].J Clin Invest,2019,129(3):972-987.
[15] KAWAHARA R,NIWA Y,SIMIZU S.Integrin β1 is an essential factor in vasculogenic mimicry of human cancer cells[J].Cancer Sci,2018,109(8):2490-2496.
[16] 徐廉松,黄富豪,张语涵,等.川芎通过多成分、多靶点、多信号通路和多生物学功能发挥抗肺癌脑转移作用[J].南方医科大学学报,2021,41(9):1319-1328.
[17] 付裕豪,邱学佳,李宵,等.第三代EGFR-TKIs在晚期非小细胞肺癌中的遴选量化评估[J].中国医院药学杂志,2023,43(1):21-27.
[18] TODARO M,GAGGIANESI M,CATALANO V,et al.CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis[J].Cell Stem Cell,2014,14(3):342-356.
[19] SAWANT S,GOKULAN R,DONGRE H,et al.Prognostic role of Oct4,CD44 and c-Myc in radio-chemo-resistant oral cancer patients and their tumourigenic potential in immunodeficient mice[J].Clin Oral Investig,2016,20(1):43-56.
[20] 孟唤男,郭晓冬,周小翠,等.骨痛灵方通过抑制整合素αvβ3通路缓解肺癌骨转移癌性疼痛[J].中国疼痛医学杂志,2023,29(2):92-100.
[21] 刘梦瑶,苟理尧,夏菁,等.整合素β2促进人乳腺癌细胞MCF-7的迁移、侵袭与粘附[J].基因组学与应用生物学,2019,38(1):313-319.
[22] 张曦,雷光焰,张新伟.Musashi2基因在肺鳞癌中的表达及其对Notch1信号通路介导的CD44v6(+)肺癌干细胞维持的研究[J].广西医科大学学报,2021,38(6):1067-1076.
[23] 张春节,张志远.免疫组化法检测非小细胞肺癌组织中CD44v6、Ki-67的表达与患者临床病理特征及转移的关系[J].内蒙古师范大学学报(自然科学汉文版),2020,49(1):72-77.
[24] 李蒙,刘槟,燕晓茹,等.不同氧环境下川芎和丹参对肺癌干细胞样细胞血管生成拟态的作用[J].辽宁中医杂志,2021,48(3):151-154.
[25] 郭秀伟,张培彤,王耀焓,等.川芎对肺癌干细胞样PG细胞荷瘤裸鼠手术前后HIF-1α表达的影响[J].辽宁中医杂志,2017,44(5):1070-1074.

相似文献/References:

[1]陈正浤,雷光焰△,韩 乐,等.三氧化二砷抑制人肺癌细胞NCI-H460增殖并促进其凋亡实验研究*[J].陕西医学杂志,2020,49(10):1219.[doi:DOI:10.3969/j.issn.1000-7377.2020.10.005]
[2]何建春,李煜华.纵隔型肺癌MRI、CT影像学特征及诊断价值对比研究*[J].陕西医学杂志,2020,49(10):1232.[doi:DOI:10.3969/j.issn.1000-7377.2020.10.008]
　HE Jianchun,LI Yuhua..Comparative study on MRI and CT features and diagnostic value for mediastinal lung cancer[J].,2020,49(4):1232.[doi:DOI:10.3969/j.issn.1000-7377.2020.10.008]
[3]荆冠军,赵一鉴△.阿帕替尼治疗晚期肺癌对患者血清肿瘤标志物的影响研究[J].陕西医学杂志,2020,49(10):1351.[doi:DOI:10.3969/j.issn.1000-7377.2020.10.040]
　JING Guanjun,ZHAO Yijian..Effect of apatinib on serum tumor markers in patients with advanced lung cancer[J].,2020,49(4):1351.[doi:DOI:10.3969/j.issn.1000-7377.2020.10.040]
[4]张 珊,任小平,田应选.老年肺癌患者心理痛苦现状调查及影响因素分析[J].陕西医学杂志,2021,50(2):245.[doi:DOI:10.3969/j.issn.1000-7377.2021.02.030]
　ZHANG Shan,REN Xiaoping,TIAN Yingxuan.Investigation on psychological distress status of elderly patients with lung cancerand analysis of its influencing factors[J].,2021,50(4):245.[doi:DOI:10.3969/j.issn.1000-7377.2021.02.030]
[5]刘振宇,王宏伟.小鼠原位肺癌模型构建及在放射治疗研究中的意义[J].陕西医学杂志,2022,51(7):791.[doi:DOI:10.3969/j.issn.1000-7377.2022.07.005]
　LIU Zhenyu,WANG Hongwei.Establishment of mouse model of lung cancer in situ and its significance in study of radiation therapy research[J].,2022,51(4):791.[doi:DOI:10.3969/j.issn.1000-7377.2022.07.005]
[6]刘 丽,武愫斌.双氧化酶成熟因子2基因检测在肺癌患者淋巴结转移及预后预测中的价值研究[J].陕西医学杂志,2022,51(8):1029.[doi:DOI:10.3969/j.issn.1000-7377.2022.08.029]
　LIU Li,WU Subin.Value of DUOXA2 level in predicting lymph node metastasis and prognosis of lung cancer patients[J].,2022,51(4):1029.[doi:DOI:10.3969/j.issn.1000-7377.2022.08.029]
[7]代 佳,李 林,贺 林,等.中性粒细胞/淋巴细胞比值、血小板/淋巴细胞比值与肺癌早期诊断以及临床病理特征相关性分析[J].陕西医学杂志,2023,52(4):468.[doi:DOI:10.3969/j.issn.1000-7377.2023.04.024]
[8]王松海,凡 瑾,陈 捷.红景天苷对Lewis 肺癌小鼠恶性胸腔积液的抑制作用及其对免疫功能的影响[J].陕西医学杂志,2024,(1):37.[doi:DOI:10.3969/j.issn.1000-7377.2024.01.008]
　WANG Songhai,FAN Jin,CHEN Jie.Inhibitory effect of SALidroside on malignant pleural effusion and its effect on immune function in mice with Lewis lung cancer[J].,2024,(4):37.[doi:DOI:10.3969/j.issn.1000-7377.2024.01.008]

备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81673797)
更新日期/Last Update: 2024-04-07