[1]朱 婧,郭建丽.氙气预处理对心肌缺血再灌注损伤大鼠心肌细胞保护作用研究[J].陕西医学杂志,2019,(1):3-5，31.
　ZU Jing,GUO Jianli..Protective effect of hernia preconditioning on myocardial cells in rats with myocardial ischemia reperfusion injury[J].,2019,(1):3-5，31.
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氙气预处理对心肌缺血再灌注损伤大鼠心肌细胞保护作用研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2019年1期
页码:
3-5，31
栏目:
基础研究
出版日期:
2019-01-05

文章信息/Info

Title:
Protective effect of hernia preconditioning on myocardial cells in rats with myocardial ischemia reperfusion injury
文章编号:
DOI:10.3969/j.issn.10007377.2019.01.001
作者:
朱 婧1; 郭建丽2
1.陕西省人民医院麻醉科（西安710068）;2.内蒙古鄂尔多斯市中心医院麻醉科（鄂尔多斯 017000）
Author(s):
ZU Jing; GUO Jianli.
Anaesthesiology Department of Shaanxi Provincial People’s Hospital(Xi’an 710068)
关键词:
心肌缺血再灌注; 心肌损伤; 麻醉预处理; 氙气; 细胞凋亡; 氧化应激
Keywords:
Key words Myocardial ischemiareperfusion; Myocardial injury; Anesthesia preconditioning; Helium; Apoptosis; Oxidative stress
分类号:
R542.2
文献标志码:
A
摘要:
摘 要 目的：通过观察氙气预处理对心肌缺血再灌注损伤（MIRI）大鼠心肌梗死范围、心肌细胞凋亡指数、心肌组织酶类及氧化应激反应水平的影响，探讨氙气预处理对MIRI大鼠心肌细胞的保护作用及机制。方法：随机抽签方式将64只雄性SD大鼠均分为四组。假手术组（S组）仅做穿线但不结扎；MIRI组（模型组）阻断左冠状动脉前降支，使大鼠缺血30 min，恢复灌注120 min； 0.5 MAC疝气预处理+心肌I/R组（0.5 XR组）和1 MAC疝气预处理+心肌I/R组（1 XR组）分别采用0.5 MAC氙气和1 MAC氙气预处理大鼠心脏后，缺血30 min，恢复灌注120 min。灌注120 min后，测定各组大鼠心肌梗死范围、心肌细胞凋亡指数、丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSHPX）含量及血清中血清肌酸激酶（CK）、肌酸激酶同工酶（CKMB）、一氧化氮合酶（eNOS）活性。结果：与S组相比，模型组心肌梗死范围明显增大，心肌细胞凋亡指数显著升高，心肌组织中SOD、GSHPX和血清eNOS活性显著下降，MDA含量显著增加，血清CK、CKMB活性显著增强（P＜0.01）；与模型组比，0.5 XR组和1 XR组大鼠心肌梗死范围显著缩小，心肌细胞凋亡指数显著下降，心肌组织中SOD、GSHPX和血清eNOS活性显著增强， MDA含量显著下降，血清CK、CKMB活性显著减小（P＜0.05）。但0.5 XR组和1 XR组大鼠上述各指标间比较差异无统计学意义（P＞0.05）。结论：氙气预处理对MIRI大鼠心肌组织及细胞具有保护作用，其作用机制可能与抑制机体心肌组织氧化应激反应有关。
Abstract:
Abstract Objective: To observe the effects of hernia preconditioning on myocardial infarction size, myocardial apoptosis index, myocardial enzymes and oxidative stress in rats with myocardial ischemiareperfusion (MIRI) injury, and to investigate the effects of hernia preconditioning on MIRIinduced rat myocardium and the mechanism.Methods: 64 male SpragueDawley rats were divided into 4 groups by random sampling. The sham operation group (S group) only threaded but did not ligature; the MIRI group (model group) blocked the left anterior descending coronary artery, and the rats were ischemia for 30 min, and the perfusion was restored for 120 min; 0.5 XR group and 1 XR group were pretreated with 0.5 MAC helium and 1 MAC helium, respectively, and ischemia was 30 min, and perfusion was continued for 120 min. After 120 min of perfusion, the myocardial infarct size, cardiomyocyte apoptosis index, MDA, SOD, GSHPXcontent and serum CK, CKMB and eNOS activities were measured.Results: Compared with the S group, the myocardial infarction range of the model group was significantly increased, the apoptotic index of cardiomyocytes was significantly increased, the activity of SOD, GSHPXand serum eNOS in myocardial tissue was significantly decreased, the content of MDA was significantly increased, and the activity of serum CK and CKMB were significantly increased (P＜0.01). Compared with the model group, the myocardial infarction range of the 0.5 XR group and the 1 XR group was significantly reduced, the apoptotic index of myocardial cells was significantly decreased, the activities of SOD, GSHPX and serum eNOS were significantly increased, and the content of MDA was significantly decreased,the activity of serum CK,CKMB was significantly reduced(P＜0.05). However, there was no significant difference between the above indexes in 0.5 XR group and 1 XR group (P＞0.05).Conclusion: Helium preconditioning has protective effects on myocardial tissue and cells in rats with MIRI injury, and its mechanism may be related to inhibition of oxidative stress in myocardial tissue.

参考文献/References:

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更新日期/Last Update: 2019-01-22