[1]彭潇,伍星,陈宏,等.槲皮素通过促进M2型巨噬细胞极化改善膝骨性关节炎大鼠滑膜炎症实验研究[J].陕西医学杂志,2026,(6):775-780.[doi:DOI:10.3969/j.issn.1000-7377.2026.06.009]
　PENG Xiao,WU Xing,CHEN Hong,et al.Quercetin improves synovial inflammation in KOA rats by promoting M2-type macrophage polarization[J].,2026,(6):775-780.[doi:DOI:10.3969/j.issn.1000-7377.2026.06.009]
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槲皮素通过促进M2型巨噬细胞极化改善膝骨性关节炎大鼠滑膜炎症实验研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2026年6期
页码:
775-780
栏目:
基础研究
出版日期:
2026-06-05

文章信息/Info

Title:
Quercetin improves synovial inflammation in KOA rats by promoting M2-type macrophage polarization
作者:
彭潇; 伍星; 陈宏; 何瀚威; 邓尚斌; 朱毅琳
（长沙市中医医院 长沙市第八医院骨伤科，湖南 长沙 410000）
Author(s):
PENG Xiao; WU Xing; CHEN Hong; HE Hanwei; DENG Shangbin; ZHU Yilin
(Department of Orthopedics and Traumatology，Changsha Hospital of Traditional Chinese Medicine，Changsha 410000，China)
关键词:
膝骨性关节炎; 槲皮素; M2型巨噬细胞极化; 滑膜炎症; 大鼠
Keywords:
Knee osteoarthritis; Quercetin; M2 macrophage polarization; Synovial inflammation; Rat
分类号:
R 285.5
DOI:
DOI:10.3969/j.issn.1000-7377.2026.06.009
文献标志码:
A
摘要:
目的：探讨槲皮素对膝骨性关节炎（KOA）大鼠滑膜炎症的作用及机制。方法：建立KOA模型大鼠，随机分为假手术组、模型组、槲皮素低剂量组（50 mg/kg）、槲皮素高剂量组（100 mg/kg）和塞来昔布组（20 mg/kg），每组10只。各组大鼠每日灌胃相应药物1次，持续4周。检测各组大鼠关节痛行为学；HE染色观察关节组织病理变化；酶联免疫吸附试验（ELISA）检测血清和关节液白细胞介素（IL）-6、单核细胞趋化蛋白-1（MCP-1）、IL-10水平；免疫组化染色检测滑膜组织肿瘤坏死因子-α（TNF-α）、环氧化酶-2（COX-2）水平；Western blot检测滑膜组织诱导型一氧化氮合酶（iNOS）、CD68、精氨酸酶1（Arg1）、CD206蛋白表达。结果：与假手术组比较，模型组大鼠机械痛阈值、热敏痛阈值降低（均P<0.05）；血清和关节液IL-6、MCP-1水平升高，IL-10水平降低（均P<0.05）；膝关节组织中软骨退变伴滑膜炎性增生，且滑膜组织TNF-α、COX-2、iNOS、CD68蛋白水平升高，Arg1、CD206蛋白水平降低（均P<0.05）。与假手术组比较，槲皮素低、高剂量组和塞来昔布组大鼠机械痛阈值、热敏痛阈值升高（均P<0.05）；血清和关节液IL-6、MCP-1水平降低，IL-10水平升高（均P<0.05）；膝关节组织病理损伤减轻，且滑膜组织TNF-α、COX-2、iNOS、CD68蛋白水平降低，Arg1、CD206蛋白水平升高（均P<0.05）。结论：槲皮素可缓解KOA大鼠关节疼痛症状，减轻其滑膜炎症，其机制可能与促进M2型巨噬细胞极化有关。
Abstract:
Objective:To investigate the effects and mechanism of quercetin on synovial inflammation in rats with knee osteoarthritis (KOA).Methods：A rat model of KOA was established and randomly divided into the following groups (n=10 each)：sham-operated group，model group，low-dose quercetin group (50 mg/kg)，high-dose quercetin group (100 mg/kg)，and celecoxib group (20 mg/kg).All groups received daily intragastric administration of the respective agents for 4 weeks.Behavioral assessments of joint pain were conducted.Histopathological alterations in joint tissues were visualized via HE staining.Interleukin-6 (IL-6)，monocyte chemoattractant protein-1 (MCP-1)，and IL-10 concentrations in serum and synovial fluid were quantified by ELISA.Synovial protein expression was profiled using immunohistochemistry and Western blot.Specifically，immunohistochemistry was utilized for the detection of tumor necrosis factor-α (TNF-α) and cyclooxygenase-2 (COX-2)，whereas Western blot was used for the quantification of inducible nitric oxide synthase (iNOS)，CD68，arginase1 (Arg1)，and CD206.Results:Compared with the sham group，KOA model rats exhibited decreased mechanical and thermal pain thresholds (all P<0.05);IL-6 and MCP-1 levels were elevated and IL-10 level was reduced in serum and synovial fluid (all P<0.05);cartilage degeneration was accompanied by synovial inflammatory hyperplasia;synovial expression of TNF-α，COX-2，iNOS，and CD68 proteins were increased，along with decreased Arg1 and CD206 proteins (all P<0.05).Relative to the model group，both low- and high-dose quercetin groups and celecoxib group reversed these changes：mechanical and thermal pain thresholds were increased (all P<0.05));IL-6 and MCP-1 levels in serum and synovial fluid were decreased while IL-10 level was increased (all P<0.05);histopathological damage was alleviated;and TNF-α，COX-2，iNOS，and CD68 protein levels in synovial were reduced，whereas Arg1 and CD206 protein levels were elevated (all P<0.05).Conclusion:Quercetin alleviates joint pain and attenuates synovial inflammation in KOA rats，possibly by promoting M2 macrophage polarization.

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备注/Memo

备注/Memo:
湖南省卫生健康委科研项目（W20243270）
更新日期/Last Update: 2026-06-05