[1]丁思航,田蕾.TET1过表达对人肝癌HepG2细胞增殖和侵袭及裸鼠移植瘤生长的影响实验研究[J].陕西医学杂志,2026,(6):736-741.[doi:DOI:10.3969/j.issn.1000-7377.2026.06.003]
　DING Sihang,TIAN Lei.Effects of TET1 overexpression on proliferation and invasion of human hepatocellular carcinoma HepG2 cells and growth of transplanted tumor in nude mice[J].,2026,(6):736-741.[doi:DOI:10.3969/j.issn.1000-7377.2026.06.003]
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TET1过表达对人肝癌HepG2细胞增殖和侵袭及裸鼠移植瘤生长的影响实验研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2026年6期
页码:
736-741
栏目:
基础研究
出版日期:
2026-06-05

文章信息/Info

Title:
Effects of TET1 overexpression on proliferation and invasion of human hepatocellular carcinoma HepG2 cells and growth of transplanted tumor in nude mice
作者:
丁思航; 田蕾
（锦州医科大学附属第一医院消化内科，辽宁 锦州 121000）
Author(s):
DING Sihang; TIAN Lei
(Department of Gastroenterology，the First Hospital of Jinzhou Medical University，Jinzhou 121000，China)
关键词:
肝癌; 甲基胞嘧啶双加氧酶1; HepG2细胞; 增殖; 侵袭; 迁移; 裸鼠
Keywords:
Hepatocellular carcinoma; Ten-eleven translocation methylcytosine di-oxygenase 1; HepG2 cells; Proliferation; Invasion; Migration; Nude mice
分类号:
R 735.7
DOI:
DOI:10.3969/j.issn.1000-7377.2026.06.003
文献标志码:
A
摘要:
目的：探讨甲基胞嘧啶双加氧酶1（TET1）过表达对人肝癌HepG2细胞增殖和侵袭及裸鼠移植瘤生长的影响。方法：选择人肝癌HepG2细胞，分为空白组（无细胞处理）、阴性对照组（转染空质粒pLVX-MYRF）和TET1过表达组（转染重组质粒pLVX-MYRF-TET1）。实时荧光定量PCR（RT-qPCR）和Western blot分别检测各组HepG2细胞TET1 mRNA和蛋白表达。CCK-8法检测各组HepG2细胞增殖能力。HepG2细胞侵袭和迁移能力以Transwell实验、细胞划痕实检测。构建皮下移植瘤模型评估成瘤能力。结果：TET1过表达组TET1 mRNA和蛋白表达高于空白组和阴性对照组（均P＜0.05）。TET1过表达组24、48、72 h细胞增殖率低于空白组和阴性对照组（均P＜0.05）。TET1过表达组HepG2细胞侵袭、迁移能力低于空白组和阴性对照组（均P＜0.05）。TET1过表达组裸鼠肿瘤体积、肿瘤重量低于空白组和阴性对照组（均P＜0.05）。结论：TET1过表达可抑制人肝癌HepG2细胞增殖、侵袭、迁移及裸鼠肝癌移植瘤生长。
Abstract:
Objective:To investigate the effects of Ten-eleven translocation methylcytosine di-oxygenase 1 (TET1) overexpression on the proliferation and invasion of human hepatocellular carcinoma HepG2 cells and the growth of transplanted tumor in nude mice.Methods：Human hepatocellular carcinoma HepG2 cells were selected and divided into a blank group (without treatment)，a negative control group (transfected with the empty plasmid pLVX-MYRF)，and a TET1 overexpression group (transfected with the recombinant plasmid pLVX-MYRF-TET1).RT-qPCR and Western blot were used to detect the expression of TET1 mRNA and protein in HepG2 cells of each group.The proliferation ability was detected by CCK-8 assay.Transwell assay and cell scratch assay were used to detect the invasion and migration abilities.A subcutaneous tumor transplantation model was constructed to evaluate the tumor formation ability.Results:The TET1 overexpression group had significantly higher mRNA and protein levels of TET1 than blank group and the negative control group (all P<0.05).The proliferation rates of cells in TET1 overexpression group at 24，48 and 72 hours were lower than those in blank group and negative control group (all P<0.05).TET1 overexpression group had lower invasion and migration abilities than blank group and negative control group (all P<0.05).The TET1 overexpression group had lower tumor volume and weight than blank group and negative control group (all P<0.05).Conclusion:Overexpression of TET1 can inhibit the proliferation，invasion，migration of human liver cancer HepG2 cells，as well as the growth of liver cancer transplanted tumors in nude mice.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目（81902452）
更新日期/Last Update: 2026-06-05