[1]赵 惠,代路勤,王晓丹,等.PGE2对人子宫内膜间质细胞迁移的影响及作用机制实验研究[J].陕西医学杂志,2026,(5):600-604,610.[doi:DOI:10.3969/j.issn.1000-7377.2026.05.004]
　ZHAO Hui,DAI Luqin,WANG Xiaodan,et al.Effect of PGE2 on the migration of human endometrial stromal cells and its mechanism[J].,2026,(5):600-604,610.[doi:DOI:10.3969/j.issn.1000-7377.2026.05.004]
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PGE2对人子宫内膜间质细胞迁移的影响及作用机制实验研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2026年5期
页码:
600-604,610
栏目:
基础研究
出版日期:
2026-05-05

文章信息/Info

Title:
Effect of PGE2 on the migration of human endometrial stromal cells and its mechanism
作者:
赵 惠; 代路勤; 王晓丹; 玛依拉·吾守尔; 巴登才次克
（巴音郭楞蒙古自治州人民医院产科，新疆 库尔勒 841000）
Author(s):
ZHAO Hui; DAI Luqin; WANG Xiaodan; MAYILA Wushouer; BADENG Caicike
(Department of Obstetrics,Bayingolin Mongolian Autonomous Prefecture People’s Hospital,Korla 841000,China)
关键词:
前列腺素E2; 人子宫内膜间质细胞; 核苷酸寡聚化结构域样受体热蛋白结构域相关蛋白3/半胱氨酸天冬氨酸蛋白酶1/白细胞介素-1β通路; 迁移; 缩宫素
Keywords:
Prostaglandin E2; Human endometrial stromal cells; NLRP3/Caspase-1/IL-1β pathway; Migration; Oxytocin
分类号:
R36
DOI:
DOI:10.3969/j.issn.1000-7377.2026.05.004
文献标志码:
A
摘要:
目的：探讨前列腺素E2（PGE2）对人子宫内膜间质细胞（hESCs）迁移的影响及其可能的作用机制。方法：选取hESCs细胞，随机分为对照组（加入等量培养基，无其他处理）、PGE2组（加入终浓度25 μmol/L PGE2）、缩宫素组（加入终浓度10 nmol/L缩宫素）、PGE2+核苷酸寡聚化结构域样受体热蛋白结构域相关蛋白3（NLRP3）抑制剂组（加入终浓度25 μmol/L PGE2+10 μmol/L NLRP3抑制剂MCC950）、PGE2+半胱氨酸天冬氨酸蛋白酶1（Caspase-1）抑制剂组（加入终浓度25 μmol/L PGE2+20 μmol/L Caspase-1抑制剂VX-765）。ELISA法检测各组hESCs细胞PGE2水平，细胞划痕实验检测各组细胞迁移率，RT-qPCR检测各组细胞中NLRP3、cleaved Caspase-1、白细胞介素-1β（IL-1β）、基质金属蛋白酶-2（MMP-2）、MMP-9 mRNA表达，Western blot检测各组细胞中NLRP3、cleaved Caspase-1、IL-1β、MMP-2、MMP-9蛋白表达。结果：PGE2组细胞迁移率、细胞中PGE2水平及NLRP3、cleaved Caspase-1、IL-1β、MMP-2、MMP-9 mRNA及蛋白表达水平高于对照组、缩宫素组，缩宫素组高于对照组（均P＜0.05）。PGE2+NLRP3抑制剂组、PGE2+Caspase-1抑制剂组上述指标低于PGE2组（均P＜0.05）。结论：PGE2可促进hESCs细胞迁移，其作用机制可能与激活NLRP3/Caspase-1/IL-1β通路有关。
Abstract:
Objective:To investigate the effect of prostaglandin E2 (PGE2) on the migration of human endometrial stromal cells (hESCs) and its possible mechanism.Methods：hESCs were randomly divided into a control group (treated with an equal volume of medium without other intervention),a PGE2 group (treated with 25 μmol/L PGE2),an oxytocin group (treated with 10 nmol/L oxytocin),a PGE2+NOD-like receptor family pyrin domain-containing protein 3 (NLRP3） inhibitor group (treated with 25 μmol/L PGE2+10 μmol/L MCC950,an inhibitor of NLRP3),and a PGE2+Caspase-1 inhibitor group (treated with 25 μmol/L PGE2+20 μmol/L VX-765,an inhibitor of Caspase-1).The level of PGE2 in hESCs was detected by ELISA.The cell migration rate was measured by wound-healing assay.The mRNA expressions of NLRP3,cleaved Caspase-1,interleukin-1β (IL-1β),matrix metalloproteinase-2 (MMP-2),and MMP-9 were determined by RT-qPCR.The protein expressions of NLRP3,cleaved Caspase-1,IL-1β,MMP-2,and MMP-9 were detected by Western blot.Results:The cell migration rate,intracellular PGE2 level,and mRNA and protein expressions of NLRP3,cleaved Caspase-1,IL-1β,MMP-2,and MMP-9 in the PGE2 group were higher than those in the control group and the oxytocin group,and those in the oxytocin group were higher than those in the control group (all P<0.05).The above indicators in the PGE2+NLRP3 inhibitor group and the PGE2+Caspase-1 inhibitor group were lower than those in the PGE2 group (all P<0.05).Conclusion:PGE2 can promote the migration of hESCs,and its mechanism may be related to the activation of the NLRP3/Caspase-1/IL-1β pathway.

参考文献/References:

[1]李淑真,韩素新,高伟,等.控释地诺前列酮栓用于妊娠晚期引产促宫颈成熟的疗效观察[J].中国妇幼保健,2019,34(3):495-498.
[2]王志先,杨波,董俊婵,等.双球囊扩张与药物治疗用于足月妊娠促宫颈成熟的临床效果比较研究[J].临床误诊误治,2018,31(4):93-96.
[3]陈冰.601例缩宫素催产引产结果分析[J].中国妇幼保健,2022,37(8):1447-1449.
[4]李娟,钟惠敏.宫颈成熟度评估及促宫颈成熟方法的研究进展[J].医学综述,2024,30(14):1711-1716.
[5]张多多,桂婷,张婉玉,等.过氧化氢通过影响细胞骨架、活性氧和线粒体功能诱发子宫内膜间质细胞的衰老[J].生殖医学杂志,2025,34(9):1229-1237.
[6]唐文波,王霖霖,杨雪,等.miR-455对子宫内膜间质细胞氧化应激的调控作用及相关机制研究[J].浙江医学,2020,42(18):1935-1939.
[7]HEWITT S C,ORELLANA F,MARQUARDT R M,et al.Transcriptional coregulator ZMIZ1 modulates estrogen responses that are essential for healthy endometrial function[J].J Clin Invest,2025,135(23):193212.
[8]周志鹏,韦维,陈丽,等.安胃汤对CAG大鼠NLRP3/Caspase-1/IL-1β信号轴细胞焦亡调控研究[J].时珍国医国药,2022,33(12):2885-2888.
[9]黄怡.PGE2经NLRP3/caspase-1/IL-1β途径诱导细胞焦亡促进hEM15A迁移的机制研究[D].兰州:兰州大学,2023.
[10]王露,李丽.足月妊娠不同促宫颈成熟及引产方式研究进展[J].中国现代医生,2022,60(24):116-118.
[11]王秋霞,杨丽杰.足月妊娠促宫颈成熟方法的研究进展[J].中国妇幼保健,2018,33(21):5031-5033.
[12]ELSAMANI MOHAMED S A,EL HASSAN HAMID M H.Comparative effectiveness,safety,and cost-effectiveness of labour induction methods:A systematic review[J].Cureus,2025,17(9):93382.
[13]GUPTA J K,MAHER A,STUBBS C,et al.A randomized trial of synthetic osmotic cervical dilator for induction of labor vs dinoprostone vaginal insert[J].Am J Obstet Gynecol MFM,2022,4(4):100628.
[14]CHOO S N,KANNEGANTI A,ABDUL AZIZ M N D B,et al.MEchanical DIlatation of the Cervix-in a Scarred uterus (MEDICS):The study protocol of a randomised controlled trial comparing a single cervical catheter balloon and prostaglandin PGE2 for cervical ripening and labour induction following caesarean delivery[J].BMJ Open,2019,9(11):e028896.
[15]ELHESSY H M,KHATTAB B A,BADAWY A M,et al.Thymoquinone attenuates aluminum chloride-induced testicular injury by inhibiting NLRP3/Caspase 1/IL-1β inflammasome signaling and polarizing the macrophages toward anti-inflammatory M2 phenotype[J].Cells,2025,14(23):1906.
[16]张伟强,赵伟,刘晨,等.基于NLRP3/Caspase-1信号通路探讨虾青素对盐敏感性高血压模型大鼠肾脏的保护作用[J].实用心脑肺血管病杂志,2026,34(2):79-83.
[17]钟燕波,朱昀恒.宫颈癌患者血清核苷酸结合寡聚化结构域样受体蛋白3炎症小体和相关细胞因子表达及临床意义[J].临床误诊误治,2024,37(15):35-40.
[18]蔡猛,张永宁.三七总皂苷调控TLR4/NLRP3/Caspase-1信号通路对骨关节炎大鼠软骨细胞焦亡的影响[J].中医药信息,2023,40(2):11-17.
[19]何星灵,李姿儒,张小娇,等.清化养肺颗粒通过调控NLRP3/Caspase-1/GSDMD信号通路抑制肺纤维化巨噬细胞焦亡的机制[J].中华中医药杂志,2025,40(5):2567-2573.
[20]袁翠,何银艳,卢伟,等.胃镜活检组织NLRP3、Caspase-1表达与胃癌分化程度及预后关系研究[J].陕西医学杂志,2025,54(9):1264-1269,1284.
[21]陈臣,李倩,刘涛,等.大黄酸调控炎症小体活化抑制急性脑出血大鼠小胶质细胞焦亡的机制研究[J].陕西医学杂志,2025,54(10):1316-1323.
[22]亢雪峰,马兰,钟伟兰,等.保产无忧散对孕晚期大鼠Cx-43基因表达及宫颈成熟相关指标的影响[J].陕西中医,2022,43(10):1364-1368.
[23]魏丹,周永红,郭瑜,等.早产与炎症反应相关机制的研究进展[J].中国医刊,2024,59(5):485-488.
[24]焦翠翠,张雯昕,孙丽洪,等.鞘内注射瞬时受体电位香草酸受体通道1阻滞剂对宫颈扩张性内脏痛反应的影响[J].中华医学杂志,2019,99(13):1018-1023.
[25]DU M,WANG Y,LIU Z,et al.Effects of IL-1β on MMP-9 expression in cementoblast-derived cell line and MMP-mediated degradation of type Ⅰ collagen[J].Inflammation,2019,42(2):413-425.

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备注/Memo

备注/Memo:
新疆维吾尔自治区卫生健康科技计划项目（2025001FYJKSCZLKJZX652829652）
更新日期/Last Update: 2026-05-05