[1]翟天元,曹金龙,郭凌宇,等.良性前列腺增生间质细胞通过分泌TGF-β1破坏前列腺腺上皮屏障[J].陕西医学杂志,2026,(5):593-599.[doi:DOI:10.3969/j.issn.1000-7377.2026.05.003]
　ZHAI Tianyuan,CAO Jinlong,GUO Lingyu,et al.Stromal cells in benign prostatic hyperplasia disrupt the prostatic luminal epithelial barrier by secreting TGF-β1[J].,2026,(5):593-599.[doi:DOI:10.3969/j.issn.1000-7377.2026.05.003]
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良性前列腺增生间质细胞通过分泌TGF-β1破坏前列腺腺上皮屏障

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2026年5期
页码:
593-599
栏目:
基础研究
出版日期:
2026-05-05

文章信息/Info

Title:
Stromal cells in benign prostatic hyperplasia disrupt the prostatic luminal epithelial barrier by secreting TGF-β1
作者:
翟天元; 曹金龙; 郭凌宇; 薛 力; 李 峰
（西安交通大学第二附属医院泌尿外科，陕西 西安 710004）
Author(s):
ZHAI Tianyuan; CAO Jinlong; GUO Lingyu; XUE Li; LI Feng
(Department of Urology,the Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,China)
关键词:
良性前列腺增生; 转化生长因子-β1; E-钙黏蛋白; 紧密连接蛋白-1; 腺上皮屏障; 间质-上皮相互作用
Keywords:
Benign prostatic hyperplasia; Transforming growth factor-β1; E-Cadherin; Claudin-1; Luminal epithelial barrier; Stromal epithelial interaction
分类号:
R36
DOI:
DOI:10.3969/j.issn.1000-7377.2026.05.003
文献标志码:
A
摘要:
目的：探讨良性前列腺增生（BPH）中间质细胞对前列腺腺上皮屏障的影响及其可能的机制。方法：选取人正常前列腺间质永生化细胞系WPMY-1和良性前列腺上皮细胞系BHPrE-1。将BHPrE-1细胞分为对照组和处理组，其中对照组使用BHPrE-1培养基培养，处理组使用含有不同比例WPMY-1条件培养基培养以及含或不含转化生长因子-β1（TGF-β1）抗体的BHPrE-1培养基或WPMY-1条件培养基培养。即处理组分为50% WPMY-1条件培养基组（50% WPMY-1条件培养基+50% BHPrE-1细胞培养基培养）和100% WPMY-1条件培养基组（100% WPMY-1条件培养基培养）；处理组分为WPMY-1条件培养基组（100% WPMY-1条件培养基培养）、TGF-β1抗体组（含有1 μg/ml TGF-β1抗体的BHPrE-1细胞培养基培养）、WPMY-1条件培养基+TGF-β1抗体组（含有1 μg/ml TGF-β1抗体的100% WPMY-1条件培养基培养）。采用跨上皮电阻（TER）测量仪和FITC-dextran Transwell渗透性实验检测各组TER值和荧光值；RT-qPCR和Western blot检测各组细胞中E-钙黏蛋白（E-Cadherin）和紧密连接蛋白-1（Claudin-1）mRNA及蛋白表达；ELISA法检测培养基中TGF-β1浓度。结果：与对照组比较，50%、100% WPMY-1条件培养基组TER值依次降低，荧光值依次升高（均P<0.05）。与对照组比较，50%、100% WPMY-1条件培养基组BHPrE-1细胞中E-Cadherin mRNA表达水平变化差异无统计学意义（均P>0.05），而E-Cadherin蛋白、Claudin-1 mRNA及蛋白表达水平依次降低（均P<0.05）。与BHPrE-1细胞培养基比较，WPMY-1条件培养基中TGF-β1浓度升高（P<0.05）。与对照组、TGF-β1抗体组、WPMY-1条件培养基+TGF-β1抗体组比较，WPMY-1条件培养基组TER值降低，荧光值升高（均P<0.05）。对照组、TGF-β1抗体组、WPMY-1条件培养基+TGF-β1抗体组TER值和荧光值比较差异无统计学意义（均P>0.05）。与对照组、TGF-β1抗体组、WPMY-1条件培养基+TGF-β1抗体组比较，WPMY-1条件培养基组E-Cadherin蛋白、Claudin-1 mRNA及蛋白表达水平降低（均P<0.05）。对照组、TGF-β1抗体组、WPMY-1条件培养基+TGF-β1抗体组E-Cadherin、Claudin-1 mRNA及蛋白表达水平比较差异无统计学意义（均P>0.05）。结论：BPH中间质细胞通过分泌TGF-β1下调上皮细胞中E-Cadherin及Claudin-1表达，进而破坏前列腺腺上皮屏障。
Abstract:
Objective:To investigate the effect of stromal cells on the prostatic luminal epithelial barrier in benign prostatic hyperplasia (BPH) and its potential mechanism.Methods：The human normal prostatic stromal immortalized cell line WPMY-1 and benign prostatic epithelial cell line BHPrE-1 were selected.BHPrE-1 cells were divided into a control group and treatment groups.The control group was cultured with BHPrE-1 medium,while the treatment groups were cultured with BHPrE-1 medium containing different proportions of WPMY-1 conditioned medium,or BHPrE-1 medium or WPMY-1 conditioned medium with or without transforming growth factor-β1 (TGF-β1) antibody.Specifically,the treatment groups included a 50% WPMY-1 conditioned medium group (cultured with 50% WPMY-1 conditioned medium+50% BHPrE-1 cell medium) and a 100% WPMY-1 conditioned medium group (cultured with 100% WPMY-1 conditioned medium).Other treatment groups were set as follows：a WPMY-1 conditioned medium group (cultured with 100% WPMY-1 conditioned medium),a TGF-β1 antibody group (cultured with BHPrE-1 cell medium containing 1 μg/ml TGF-β1 antibody),and a WPMY-1 conditioned medium+TGF-β1 antibody group (cultured with 100% WPMY-1 conditioned medium containing 1 μg/ml TGF-β1 antibody).Transepithelial electrical resistance (TER) meter and FITC-dextran Transwell permeability assay were used to detect TER values and fluorescence values in each group.RT-qPCR and Western blot were applied to measure the mRNA and protein expressions of E-Cadherin and Claudin-1 in cells of each group.ELISA was used to detect the concentration of TGF-β1 in the culture medium.Results:Compared with the control group,the TER values in the 50% and 100% WPMY-1 conditioned medium groups were decreased sequentially,and the fluorescence values were increased sequentially (all P<0.05).Compared with the control group,there were no significant differences in the mRNA expression levels of E-Cadherin in BHPrE-1 cells between the 50% and 100% WPMY-1 conditioned medium groups (all P>0.05),whereas the protein expression of E-Cadherin and the mRNA and protein expressions of Claudin-1 were decreased sequentially (all P<0.05).Compared with BHPrE-1 cell medium,the concentration of TGF-β1 in WPMY-1 conditioned medium was increased (P<0.05).Compared with the control group,the TGF-β1 antibody group,and the WPMY-1 conditioned medium+TGF-β1 antibody group,the TER value was decreased and the fluorescence value was increased in the WPMY-1 conditioned medium group (all P<0.05).There were no significant differences in TER values and fluorescence values among the control group,the TGF-β1 antibody group,and the WPMY-1 conditioned medium+TGF-β1 antibody group (all P>0.05).Compared with the control group,the TGF-β1 antibody group,and the WPMY-1 conditioned medium+TGF-β1 antibody group,the protein expression of E-Cadherin and the mRNA and protein expressions of Claudin-1 were decreased in the WPMY-1 conditioned medium group (all P<0.05).There were no significant differences in the mRNA and protein expressions of E-Cadherin and Claudin-1 among the control group,the TGF-β1 antibody group,and the WPMY-1 conditioned medium+TGF-β1 antibody group (all P>0.05).Conclusion:Stromal cells in BPH downregulate the expressions of E-Cadherin and Claudin-1 in epithelial cells by secreting TGF-β1,thereby disrupting the prostatic luminal epithelial barrier.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目（82100812）；陕西省自然科学基础研究计划项目（2020JQ-544）
更新日期/Last Update: 2026-05-05