[1]徐慧琛,侯 超.油酸对胃癌细胞增殖、迁移、侵袭及运动活性的影响实验研究[J].陕西医学杂志,2026,(5):579-586,592.[doi:DOI:10.3969/j.issn.1000-7377.2026.05.001]
　XU Huichen,HOU Chao.Effects of oleic acid on proliferation,migration,invasion and motility of gastric cancer cells[J].,2026,(5):579-586,592.[doi:DOI:10.3969/j.issn.1000-7377.2026.05.001]
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油酸对胃癌细胞增殖、迁移、侵袭及运动活性的影响实验研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2026年5期
页码:
579-586,592
栏目:
基础研究
出版日期:
2026-05-05

文章信息/Info

Title:
Effects of oleic acid on proliferation,migration,invasion and motility of gastric cancer cells
作者:
徐慧琛1; 侯 超2
[1.广州中医药大学深圳医院（福田）内分泌科，广东 深圳 518034；2.深圳市中医院肿瘤与血液病科，广东 深圳 518033]
Author(s):
XU Huichen1; HOU Chao2
(1.Department of Endocrinology,Shenzhen Hospital [Futian] of Guangzhou University of Chinese Medicine,Shenzhen 518034,China;2.Department of Oncology and Hematology,Shenzhen Traditional Chinese Medicine Hospital,Shenzhen 518033,China)
关键词:
胃癌; 油酸; 脂肪甘油三酯脂肪酶; 增殖; 迁移; 侵袭; 运动活性
Keywords:
Gastric cancer; Oleic acid; ATGL; Proliferation; Migration; Invasion; Motility
分类号:
R36
DOI:
DOI:10.3969/j.issn.1000-7377.2026.05.001
文献标志码:
A
摘要:
目的：探讨油酸对胃癌细胞增殖、迁移、侵袭及运动活性的影响。方法：选取人胃黏膜上皮细胞（GES-1）和人胃癌细胞系（AGS、HGC-27、MKN-45、MKN-28），加入不同浓度的油酸，采用油红O染色法检测细胞内脂滴形成情况，MTT法和EdU染色法评估细胞的活性及增殖情况，伤口愈合实验和Transwell实验观察细胞的迁移和侵袭能力，高内涵细胞成像技术监测细胞的实时运动，RT-qPCR和Western blot检测细胞内脂肪甘油三酯脂肪酶（ATGL）mRNA及蛋白表达水平。结果：与GES-1细胞比较，除24 h AGS细胞外，MKN-45、MKN-28、HGC-27（24、48 h）和AGS（48 h）细胞脂滴形成率升高（均P<0.05）。与0 μmol/L油酸比较，50～400 μmol/L油酸浓度下（24、48 h）MKN-45细胞存活率升高（均P<0.05）。与0 μmol/L油酸比较，50～200 μmol/L油酸浓度下（24、48 h）HGC-27细胞存活率差异无统计学意义（均P＞0.05），而300、400 μmol/L油酸浓度下（24、48 h）HGC-27细胞存活率降低（均P<0.05）。与0 μmol/L油酸比较，200 μmol/L油酸浓度下（24、48 h）MKN-45和HGC-27细胞增殖率升高（均P<0.05）。与0 μmol/L油酸比较，除24 h MKN-45细胞外，200 μmol/L油酸浓度下MKN-45（48 h）和HGC-27（24、48 h）细胞迁移率升高（均P<0.05）。与0 μmol/L油酸比较，除24 h HGC-27细胞外，200 μmol/L油酸浓度下MKN-45（24、48 h）和HGC-27（48 h）细胞迁移数目升高（均P<0.05）。与0 μmol/L油酸比较，除48 h HGC-27细胞外，200 μmol/L油酸浓度下MKN-45（24、48 h）和HGC-27（24 h）细胞侵袭数目升高（均P<0.05）。与0 μmol/L油酸比较，经200 μmol/L油酸处理后的MKN-45和HGC-27细胞均方位移随观察时间延长呈上升趋势，HGC-27细胞实时移动速度加快（P<0.05），MKN-45和HGC-27细胞运动活性增强。与GES-1细胞比较，AGS、HGC-27、MKN-45和MKN-28细胞ATGL蛋白表达水平均有下调，但差异无统计学意义（均P＞0.05）。与GES-1细胞比较，AGS、HGC-27、MKN-45和MKN-28细胞ATGL mRNA表达水平均有下调，其中HGC-27与MKN-28细胞ATGL mRNA表达水平差异有统计学意义（均P<0.05）。结论：胃癌细胞的脂滴形成能力高于正常胃上皮细胞，可能与ATGL表达下调有关。油酸能够增强胃癌细胞的增殖、迁移、侵袭能力及运动活性，但其作用具有细胞亚型特异性。
Abstract:
Objective:To investigate the effects of oleic acid on proliferation,migration,invasion and motility of gastric cancer cells.Methods：Human gastric mucosal epithelial cells (GES-1) and human gastric cancer cell lines (AGS,HGC-27,MKN-45,MKN-28) were treated with different concentrations of oleic acid.Intracellular lipid droplet formation was detected by oil red O staining.Cell viability and proliferation were evaluated by MTT assay and EdU staining.Wound healing assay and Transwell assay were used to observe cell migration and invasion.Real-time cell movement was monitored by high-content cell imaging.The mRNA and protein expression levels of adipose triglyceride lipase (ATGL) were detected by RT-qPCR and Western blot.Results:Compared with GES-1 cells,the lipid droplet formation rate was increased in MKN-45,MKN-28,HGC-27 cells at 24 and 48 hours and AGS cells at 48 hours (all P<0.05),except for AGS cells at 24 hours.Compared with 0 μmol/L oleic acid,the viability of MKN-45 cells was increased at 50-400 μmol/L oleic acid at 24 and 48 hours (all P<0.05).Compared with 0 μmol/L oleic acid,there was no significant difference in the viability of HGC-27 cells at 50-200 μmol/L oleic acid at 24 and 48 hours (all P>0.05),while the viability was decreased at 300 and 400 μmol/L oleic acid at 24 and 48 hours (all P<0.05).Compared with 0 μmol/L oleic acid,the proliferation rate of MKN-45 and HGC-27 cells was increased at 200 μmol/L oleic acid at 24 and 48 hours (all P<0.05).Compared with 0 μmol/L oleic acid,the migration rate was increased in MKN-45 cells at 48 hours and HGC-27 cells at 24 and 48 hours treated with 200 μmol/L oleic acid (all P<0.05),except for MKN-45 cells at 24 hours.Compared with 0 μmol/L oleic acid,the number of migrated cells was increased in MKN-45 cells at 24 and 48 hours and HGC-27 cells at 48 hours treated with 200 μmol/L oleic acid (all P<0.05),except for HGC-27 cells at 24 hours.Compared with 0 μmol/L oleic acid,the number of invasive cells was increased in MKN-45 cells at 24 and 48 hours and HGC-27 cells at 24 hours treated with 200 μmol/L oleic acid (all P<0.05),except for HGC-27 cells at 48 hours.Compared with 0 μmol/L oleic acid,the mean squared displacement of MKN-45 and HGC-27 cells treated with 200 μmol/L oleic acid showed an upward trend with prolonged observation time,the real-time movement speed of HGC-27 cells was accelerated (P<0.05),and the motility of both cell lines was enhanced.Compared with GES-1 cells,the protein expression of ATGL was downregulated in AGS,HGC-27,MKN-45 and MKN-28 cells,but the differences were not statistically significant (all P>0.05).Compared with GES-1 cells,the mRNA expression of ATGL was downregulated in AGS,HGC-27,MKN-45 and MKN-28 cells,and the differences were statistically significant in HGC-27 and MKN-28 cells (all P<0.05).Conclusion:Lipid droplet formation ability is higher in gastric cancer cells than in normal gastric epithelial cells,which may be related to the downregulation of ATGL expression.Oleic acid can enhance the proliferation,migration,invasion and motility of gastric cancer cells in a cell subtype-specific manner.

参考文献/References:

[1]SUNDAR R,NAKAYAMA I,MARKAR S R,et al.Gastric cancer[J].Lancet,2025,405(10494):2087-2102.
[2]郑荣寿,陈茹,韩冰峰,等.2022 年中国恶性肿瘤流行情况分析[J].中华肿瘤杂志,2024,46(3):221-231.
[3]WANG F H,ZHANG X T,TANG L,et al.The Chinese Society of Clinical Oncology (CSCO):Clinical guidelines for the diagnosis and treatment of gastric cancer,2023[J].Cancer Commun (Lond),2024,44(1):127-172.
[4]BIAN X,LIU R,MENG Y,et al.Lipid metabolism and cancer[J].Review J Exp Med,2021,218(1):e20201606.
[5]包鑫鑫,马月,朱雅婷,等.基于脂质代谢相关基因的食管鳞癌预后预测模型构建及体外细胞实验验证[J].陕西医学杂志,2024,53(12):1587-1592,1598.
[6]ITO T,SATO K,MAEKAWA H,et al.Elevated levels of serum fatty acid synthase in patients with gastric carcinoma[J].Oncol Lett,2014,7(3):616-620.
[7]LIN A S,SHUMAN J H B,KOTNALA A,et al.Loss of corpus-specific lipids in helicobacter pylori-induced atrophic gastritis[J].mSphere,2021,6(6):e0082621.
[8]CHEN D,ZHOU X B,YAN P Y,et al.Lipid metabolism reprogramming in colorectal cancer[J].J Cell Biochem,2023,124(1):3-16.
[9]LI X Z,YAN Y,ZHANG J F,et al.Oleic acid in the absence of a PPARγ agonist increases adipogenic gene expression in bovine muscle satellite cells1[J].J Anim Sci,2019,97(10):4114-4123.
[10]GUO Z Y,LI Y J,GUO Y Y,et al.Oleic acid activates TGFβ-Smad3 signaling to promote ovarian cancer progression[J].J Ovarian Res,2025,18(1):180.
[11]CHAJES V,JENAB M,ROMIEU I,et al.Plasma phospholipid fatty acid concentrations and risk of gastric adenocarcinomas in the European Prospective Investigation into Cancer and Nutrition (EPIC-EURGAST)[J].Am J Clin Nutr,2011,94(5):1304-1313.
[12]LIN G L,LIU Q N,XIE C J,et al.Upregulated FSP1 by GPD1/1L mediated lipid droplet accumulation enhances ferroptosis resistance and peritoneal metastasis in gastric cancer[J].Cell Commun Signal,2025,23(1):132.
[13]ZHANG J,PENG J,WANG S,et al.Perilipin2-dependent lipid droplets accumulation promotes metastasis of oral squamous cell carcinoma via epithelial-mesenchymal transition[J].Cell Death Discov,2025,11(1):30.
[14]LIN Y,ZHANG X,WANG Y,et al.LPCAT2-mediated lipid droplet production supports pancreatic cancer chemoresistance and cell motility[J].Int Immunopharmacol,2024,139:112681.
[15]SHEN D,GAO Y,HUANG Q,et al.E2F1 promotes proliferation and metastasis of clear cell renal cell carcinoma via activation of SREBP1-dependent fatty acid biosynthesis[J].Cancer Lett,2021,514:48-62.
[16]MORSHED M N,FITCHEV P,ALAM M M,et al.DGAT1 inhibition enhances olaparib-induced lipotoxic apoptosis in metastatic castration-resistant prostate cancer[J].FASEB J,2025,39(15):e70925.
[17]WARREN W,OSBPRM M,YATES A,et al.The emerging role of fatty acid-binding protein 3 (FABP3) in cancer[J].Drug Discov Today,2025,30(11):104504.
[18]赵静,洪子强,苟云久.脂肪甘油三酯脂肪酶在肿瘤中的研究进展[J].临床与实验病理学杂志,2024,40(2):198-201.
[19]YIN H F,LIN W T,MO L M,et al.Adipose triglyceride lipase promotes the proliferation of colorectal cancer cells via enhancing the lipolytic pathway[J].J Cell Mol Med,2021,25(8):3963-3975.
[20]LIU M L,YU X G,LIN L Y,et al.ATGL promotes the proliferation of hepatocellular carcinoma cells via the p-AKT signaling pathway[J].J Biochem Mol Toxicol,2019,33(11):e22391.
[21]WANG Y Y,ATTANE C,MILHAS D,et al.Mammary adipocytes stimulate breast cancer invasion through metabolic remodeling of tumor cells[J].JCI Insight,2017,2(4):e87489.
[22]王梦婷,王云龙,梁梦霞,等.油酸诱导的脂滴合成对骨肉瘤细胞增殖、迁移、侵袭和上皮-间质转化的影响[J].安徽医科大学学报,2026,61(1):9-15.
[23]秦虹,张晓玉,罗旋,等.油酸激活Src基因诱导Hela细胞增殖、迁移和侵袭的研究[J].重庆医学,2021,50(18):3084-3089.
[24]YONEZAWA T,HAGA S,KOBAYASHI Y,et al.Unsaturated fatty acids promote proliferation via ERK1/2 and Akt pathway in bovine mammary epithelial cells[J].Biochem Biophys Res Commun,2008,367(4):729-735.
[25]TAHARA E.Molecular aspects of invasion and metastasis of stomach cancer[J].Verh Dtsch Ges Pathol,2000,84:43-49.
[26]JIANG H P,YU D Y,YANG P H,et al.Revealing the transcriptional heterogeneity of organ-specific metastasis in human gastric cancer using single-cell RNA Sequencing[J].Clin Transl Med,2022,12(2):e730.
[27]蒋琳,王伟,黄文泉,等.油酸抑制舌鳞癌细胞侵袭迁移能力的研究[J].江西医药,2021,56(7):923-926.
[28]吴金秀,王翠喆,张美秀,等.油酸对子宫内膜癌细胞Ishikawa生物学行为的影响及机制初探[J].石河子大学学报(自然科学版),2018,36(5):575-580.
[29]LI S,ZHOU T,LI C,et al.High metastaticgastric and breast cancer cells consume oleic acid in an AMPK dependent manner[J].PLoS One,2014,9(5):e97330.
[30]古美美,胡吉贤,张金聚,等.应用无标记高内涵成像分析技术检测乳酸对肿瘤细胞增殖与迁移的促进作用[J].广州医科大学学报,2024,52(1):1-6.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目（82305102)
更新日期/Last Update: 2026-05-05