[1]张百超,单鸿伟.臭氧联合艾蒿花粉诱发大鼠雷暴哮喘动物模型构建及评价[J].陕西医学杂志,2026,(4):467-472,499.[doi:DOI:10.3969/j.issn.1000-7377.2026.04.006]
　ZHANG Baichao,SHAN Hongwei.Establishment and evaluation of an animal model of thunderstorm asthma in rats induced by ozone combined with artemisia pollen[J].,2026,(4):467-472,499.[doi:DOI:10.3969/j.issn.1000-7377.2026.04.006]
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臭氧联合艾蒿花粉诱发大鼠雷暴哮喘动物模型构建及评价

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2026年4期
页码:
467-472,499
栏目:
基础研究
出版日期:
2026-04-05

文章信息/Info

Title:
Establishment and evaluation of an animal model of thunderstorm asthma in rats induced by ozone combined with artemisia pollen
作者:
张百超1; 单鸿伟2
（1.内蒙古医科大学第一临床医学院，内蒙古 呼和浩特 010110;2.内蒙古医科大学附属医院急诊科，内蒙古 呼和浩特 010050）
Author(s):
ZHANG Baichao1; SHAN Hongwei2
（1.First Clinical Medical College,Inner Mongolia Medical University,Hohhot 010110，China；2.Emergency Department,Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010050,China）
关键词:
臭氧; 艾蒿花粉; 过敏性鼻炎; 雷暴哮喘; 过敏性哮喘; 动物模型
Keywords:
Ozone; Artemisia pollen; Allergic rhinitis; Thunderstorm asthma; Allergic asthma; Animal model
分类号:
R 562.2
DOI:
DOI:10.3969/j.issn.1000-7377.2026.04.006
文献标志码:
A
摘要:
目的：探究通过臭氧和艾蒿花粉致敏并激发过敏性鼻炎（AR）大鼠，构建雷暴哮喘（TA）动物模型并评价。方法：使用艾蒿花粉（AP）通过间隔3 d注射致敏外加连续滴鼻5 d构建艾蒿花粉过敏性鼻炎大鼠模型，通过表观症状评分筛选（≥5分）造模成功大鼠32只，将32只大鼠分为对照组（D组，n=8）、花粉组（H组，n=8）、臭氧组（O组，n=8）、花粉臭氧组（HO组，n=8），根据分组对各组进行磷酸盐缓冲生理盐水（PBS）、艾蒿花粉、臭氧单独或联合雾化激发构建雷暴哮喘动物模型。最后一次激发前测定各组肺功能基线水平，包括支气管收缩系数、呼吸比和潮气量，最后一次激发后复测上述指标，麻醉处死取材测定血清IgE及肺组织病理，评估模型。结果：①雾化激发前后H组、O组、HO组组内比较气道阻力（收缩系数、呼吸比）明显升高（均P＜0.001），潮气量明显下降（均P＜0.01），提示支气管哮喘模型构建成功；②激发前H组与D组、O组与D组、HO组与D组组间比较气道阻力显著提高（均P＜0.05）；③激发后H组与D组、O组与D组组间比较气道阻力著提高（均P＜0.001），且HO组与D组、HO组与H组、HO组与O组组间比较气道阻力提高更为显著（均P＜0.0001）；④D组与K组比较、O组与D组比较、H组与D组、HO组与D组、H组与O组比较血清IgE显著升高（均P＜0.01），其中HO组与D组比较差异更为显著（均P＜0.0001），另外HO组与H组、HO组与O组比较血清IgE也有显著升高（均P＜0.01）；⑤D组与K组比较、H组与D组比较、O组与D组比较、HO组与D组比较、HO组与H组比较、HO组与O组比较、H组与O组比较肺组织炎性浸润均有统计学差异（均P＜0.0001）。结论：臭氧与艾蒿花粉协同加重过敏性鼻炎大鼠气道病重塑，成功构建雷暴哮喘动物模型，为雷暴哮喘研究提供基础。
Abstract:
Objective:To investigate the establishment and evaluation of a thunderstorm asthma （TA） animal model by sensitizing and challenging rats with allergic rhinitis （AR） induced by ozone and Artemisia pollen （AP）.Methods：A rat model of Artemisia polleninduced allergic rhinitis was established via intraperitoneal sensitization every 3 days combined with consecutive nasal drops for 5 days.Thirtytwo rats with successful modeling （apparent symptom score ≥ 5 points） were selected and randomly divided into four groups （n=8 each）：control group （Group D）,pollen group （Group H）,ozone group （Group O）,and pollenozone group （Group HO）.According to the grouping,the rats in each group were challenged with PBS,Artemisia pollen,ozone alone or in combination via nebulization to construct the TA animal model.Before the last challenge,the baseline pulmonary function indices of each group were measured,including bronchoconstriction coefficient,respiratory rate ratio,and tidal volume.The above indices were remeasured after the last challenge.The rats were anesthetized and sacrificed for sample collection to detect serum IgE levels and observe pulmonary histopathology,so as to evaluate the model.Results:①Intragroup comparisons showed that airway resistance （bronchoconstriction coefficient and respiratory rate ratio） was significantly increased （all P<0.001） and tidal volume was significantly decreased （all P<0.01） in Groups H,O,and HO before and after nebulization challenge,indicating the successful establishment of the bronchial asthma model.②Before challenge,intergroup comparisons revealed that airway resistance was significantly higher in Group H vs Group D,Group O vs Group D,and Group HO vs Group D （all P<0.05）.③After challenge,airway resistance was significantly elevated in Group H vs.Group D and Group O vs Group D （all P<0.001）;moreover,the increase in airway resistance was more pronounced in Group HO vs Group D,Group HO vs Group H,and Group HO vs Group O （all P<0.0001）.④Serum IgE levels were significantly higher in Group D vs Group K,Group O vs Group D,Group H vs Group D,Group HO vs Group D,and Group H vs Group O （all P<0.01）,with the most significant difference observed in Group HO vs Group D （all P<0.0001）.In aD-Dition,serum IgE levels were also significantly increased in Group HO vs Group H and Group HO vs Group O （all P<0.01）.⑤Intergroup comparisons demonstrated significant differences in pulmonary inflammatory infiltration between Group D vs Group K,Group H vs Group D,Group O vs Group D,Group HO vs Group D,Group HO vs Group H,Group HO vs Group O,and Group H vs Group O （all P<0.0001）.Conclusion:O3 and AP synergistically exacerbate airway remodeling in rats with AR,and the TA animal model was successfully established.This model provides a solid experimental foundation for further research on TA.

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备注/Memo

备注/Memo:
内蒙古自治区自然基金资助项目（2023MS03018）；内蒙古医科大学附属医院临床医学学科科研能力提升专项项目（NYFY2025LCYXXK015）；内蒙古医科大学联合项目（YKD2022LH025）
更新日期/Last Update: 2026-04-05