[1]康涛,王倩倩.丹参酮ⅡA通过抑制JNK信号通路减轻高糖诱导的周围神经炎性损伤[J].陕西医学杂志,2026,(4):462-466.[doi:DOI:10.3969/j.issn.1000-7377.2026.04.005]
　KANG Tao,WANG Qianqian.Tanshinone ⅡA alleviates high glucoseinduced peripheral neuroinflammatory injury by inhibiting the JNK signaling pathway[J].,2026,(4):462-466.[doi:DOI:10.3969/j.issn.1000-7377.2026.04.005]
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丹参酮ⅡA通过抑制JNK信号通路减轻高糖诱导的周围神经炎性损伤

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2026年4期
页码:
462-466
栏目:
基础研究
出版日期:
2026-04-05

文章信息/Info

Title:
Tanshinone ⅡA alleviates high glucoseinduced peripheral neuroinflammatory injury by inhibiting the JNK signaling pathway
作者:
康涛1; 王倩倩2
（1.陕西省人民医院神经内2科，陕西 西安 710068；2.西安交通大学第一附属医院中医科，陕西 西安 710061）
Author(s):
KANG Tao1; WANG Qianqian2
（1.Department of Neurology Ⅱ,Shaanxi Provincial People’s Hospital,Xi’an 710068,China;2.Departmentof Traditional Chinese Medicine,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,China）
关键词:
糖尿病周围神经病变; 丹参酮ⅡA; c-Jun N端激酶; 磷酸化c-Jun; 磷酸化转录激活因子2; 炎性因子
Keywords:
Diabetic peripheral neuropathy; Tanshinone ⅡA; c-Jun Nterminal Kinase; Phosphorylated c-Jun; Phosphorylated activating transcription factor 2; Inflammatory factors
分类号:
R 587.1
DOI:
DOI:10.3969/j.issn.1000-7377.2026.04.005
文献标志码:
A
摘要:
目的：糖尿病周围神经病变（DPN）与炎性反应密切相关，JNK信号通路是炎性反应的关键环节。丹参酮ⅡA（TⅡA）具有抑制炎性反应、保护神经的作用。本研究探讨TⅡA通过调控JNK信号通路干预DPN的分子机制。方法：采用RSC96细胞构建体外模型。实验设对照组、高糖组、TⅡA高、中、低组。采用CCK-8法评估TⅡA对细胞活力的影响。Western blot法检测磷酸化c-Jun氨基末端激酶（p-JNK）、磷酸化c-Jun（p-c-Jun）、磷酸化激活转录因子2（p-ATF-2）及炎症因子（IL-1β、IL-6、TNF-α）的表达水平。ELISA法检测炎性因子的含量。结果：CCK-8结果显示，TⅡA对RSC96细胞活力的影响呈现时间依赖性，随着作用时间的延长，细胞活力增强，以72 h最为显著。浓度方面，2~6 μg/ml范围内，细胞活力显著增强，之后随着浓度上升，细胞增殖活力减弱。WB结果显示，与对照组比较，高糖组p-JNK、p-c-Jun、p-ATF-2、IL-1β、IL-6和TNF-α的蛋白表达水平上调（均P<0.05）。TⅡA干预后，这些指标被显著抑制（均P<0.05）。ELISA实验显示，高糖诱导的细胞上清液中IL-1β、IL-6和TNF-α含量升高，而TⅡA可以逆转这一现象（均P<0.05）。结论：TⅡA可能通过抑制JNK信号通路减少促炎因子的释放，从而发挥对DPN的治疗作用。
Abstract:
Objective:Diabetic peripheral neuropathy （DPN） is closely associated with inflammatory responses,and the JNK signaling pathway is a key link in inflammation.Tanshinone ⅡA （TⅡA） possesses antiinflammatory and neuroprotective effects.This study aims to investigate the molecular mechanism by which TⅡA intervenes in DPN through regulating the JNK signaling pathway.Methods：An in vitro model was established using RSC96 cells.Experiments were divided into control group,high glucose group,and high/medium/low TⅡA concentration groups.The CCK-8 assay was used to evaluate the effect of TⅡA on cell viability.Western blot （WB） was used to detect the expression levels of JNK,p-JNK,p-c-Jun,p-ATF-2,and inflammatory factors （IL-1β,IL-6,TNF-α）.The content of inflammatory factors in the cell supernatant was measured by ELISA.Results:CCK-8 results showed that the effect of TⅡA on RSC96 cell viability was timedependent.Cell viability increased with prolonged action time,most significantly at 72 hours.Regarding concentration,within the range of 2~6 μg/ml,cell viability significantly increased;beyond this range,cell proliferation activity decreased with increasing concentration.WB results showed that compared with the control group,the protein expression levels of p-JNK,p-c-Jun,p-ATF-2,IL-1β,IL-6,and TNF-α were upregulated in the high glucose group （all P<0.05）.After TⅡA intervention,these indicators were significantly inhibited （all P<0.05）.ELISA experiments showed that the contents of IL-1β,IL-6,and TNF-α in the cell supernatant induced by high glucose were increased,and TⅡA reversed this phenomenon （P<0.05）.Conclusion:TⅡA may exert therapeutic effects on DPN by inhibiting the JNK signaling pathway and reducing the release of proinflammatory factors.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目（82205017）；陕西省自然科学基础研究计划项目（2020JQ515）；陕西省中医药管理局课题（2021ZZJC013）
更新日期/Last Update: 2026-04-05