[1]杨天翔,王怡,刘学起,等.RhoA/ROCK信号转导通路在原发性膝关节骨性关节炎滑膜组织中的表达及意义[J].陕西医学杂志,2026,(3):406-412.[doi:DOI:10.3969/j.issn.1000-7377.2026.03.020]
　YANG Tianxiang,WANG Yi,LIU Xueqi,et al.Expression and significance of RhoA/ROCK signal transduction pathway in synovial tissues of primary knee osteoarthritis[J].,2026,(3):406-412.[doi:DOI:10.3969/j.issn.1000-7377.2026.03.020]
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RhoA/ROCK信号转导通路在原发性膝关节骨性关节炎滑膜组织中的表达及意义

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2026年3期
页码:
406-412
栏目:
临床病理
出版日期:
2026-03-05

文章信息/Info

Title:
Expression and significance of RhoA/ROCK signal transduction pathway in synovial tissues of primary knee osteoarthritis
作者:
杨天翔1; 2; 王怡2; 刘学起1; 2; 王胤斌1; 2; 马彪1; 王若瑜1; 马亚星1; 陈德胜2
（1.宁夏医科大学第三临床医学院，宁夏 银川 750002；2.宁夏回族自治区人民医院 宁夏医科大学附属自治区人民医院，宁夏 银川 750002）
Author(s):
YANG Tianxiang1; 2; WANG Yi2; LIU Xueqi1; 2; WANG Yinbin1; 2; MA Biao1; WANG Ruoyu1; MA Yaxing1; CHEN Desheng2
(1.Third Clinical Medical College of Ningxia Medical University,Yinchuan 750002,China;2.People’s Hospital of Ningxia Hui Autonomous Region,Ningxia Medical University,Yinchuan 750002,China)
关键词:
膝关节骨性关节炎; 滑膜组织; 滑膜炎; Ras同源基因家族成员A; Rho相关蛋白激酶; 信号转导通路; 组织形态
Keywords:
Knee osteoarthritis; Synovial tissues; Synovitis; Ras homolog gene family member A; Rho-associated protein kinase; Signaling transduction pathway; Histomorphology
分类号:
R 684.3
DOI:
DOI:10.3969/j.issn.1000-7377.2026.03.020
文献标志码:
A
摘要:
目的：探讨Ras同源基因家族成员A（RhoA）/Rho相关蛋白激酶（ROCK）信号转导通路在原发性膝关节骨性关节炎（KOA）滑膜组织中的表达及意义。方法：收集30例行全膝关节置换术（TKA）的原发性KOA患者的滑膜组织作为观察组，以25例来自下肢离断术的滑膜组织作为对照组，比较两组样本的外观形态与HE染色病理改变。通过免疫组织化学（IHC）染色、蛋白质免疫印迹（Western blot）及实时荧光定量PCR（RT-qPCR）检测RhoA和ROCK蛋白及mRNA的表达变化。结果：观察组标本呈现高度增生、充血、水肿、细胞排列紊乱及空泡化细胞。对照组标本呈白色，表面光滑无水肿，细胞/基质排列有序且均匀。观察组滑膜炎病理评分及RhoA、ROCK mRNA和蛋白表达水平高于对照组（均P<0.05）。结论：KOA患者滑膜组织中RhoA和ROCK mRNA及蛋白表达水平显著提高，表明RhoA/ROCK信号通路参与了原发性KOA发病过程。
Abstract:
Objective:To investigate the expression and significance of the Ras homolog gene family member A (RhoA)/Rho-associated protein kinase (ROCK) signal transduction pathway in the synovial tissues of primary knee osteoarthritis (KOA).Methods：The synovial tissue samples obtained from 30 patients with primary KOA who underwent TKA and 25 patients who underwent lower limb amputation were set as the observation group and the control group respectively.The appearance morphology and HE staining results of the two groups of specimens were compared.The expression changes of RhoA and ROCK proteins and mRNAs were detected by IHC,Western blot,and RT-qPCR.Results:The observation group specimens showed high proliferation,congestion,oedema,unorganized cellular arrangement,and presence of vacuolated cells.The control group specimens were white with a smooth surface and non-oedematous texture,and showed organized and homogenous cell/stroma arrangement.The pathological score of synovitis in the observation group was higher than that in the control group,as well as the expression levels of RhoA,ROCK mRNA and protein (all P<0.05).Conclusion:Patients with KOA expressed high levels of RhoA and ROCK transcripts and proteins in synovial tissues,indicating that the RhoA/ROCK signaling pathway is involved in the pathogenesis of primary KOA.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目（82060408）；国家卫生健康委医疗器械使用管理课题（2025TKA007）；宁夏回族自治区东西部科技成果引进转化专项项目（2024CJE09046）；宁夏回族自治区临床医学研究所开放课题（2023KFZD01）；宁夏回族自治区留学人员创新创业项目（NRS2021-5）
更新日期/Last Update: 2026-03-05