[1]熊英琼,江铃铃,彭毓棻,等.基于磷脂酰肌醇3激酶/蛋白激酶B信号通路探究麦冬皂苷D对脑缺血再灌注损伤的神经保护作用[J].陕西医学杂志,2026,(1):35-42.[doi:DOI:10.3969/j.issn.1000-7377.2026.01.006]
　XIONG Yingqiong,JIANG Lingling,PENG Yufen,et al.Exploring the neuroprotective effect of ophiopogonin D on cerebral ischemia-reperfusion injury based on the PI3K/AKT signaling pathway[J].,2026,(1):35-42.[doi:DOI:10.3969/j.issn.1000-7377.2026.01.006]
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基于磷脂酰肌醇3激酶/蛋白激酶B信号通路探究麦冬皂苷D对脑缺血再灌注损伤的神经保护作用

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2026年1期
页码:
35-42
栏目:
基础研究
出版日期:
2026-01-05

文章信息/Info

Title:
Exploring the neuroprotective effect of ophiopogonin D on cerebral ischemia-reperfusion injury based on the PI3K/AKT signaling pathway
作者:
熊英琼1; 江铃铃2; 彭毓棻1 ; 黄招君1; 李小兵1 ; 万琛宜1
（ 1.南昌大学第一附属医院神经内科，江西 南昌 330006；2.江西中医药大学研究生院，江西 南昌330006）
Author(s):
XIONG Yingqiong1; JIANG Lingling2; PENG Yufen1; HUANG Zhaojun1; LI Xiaobing1; WAN Chenyi1
（1.Department of Neurology,First Affiliated Hospital of Nanchang University，Nanchang 330006，China;2.Graduate School of Jiangxi University of Traditional Chinese Medicine，Nanchang 330006，China）
关键词:
缺血性脑卒中; 脑缺血再灌注; 麦冬皂苷D; 神经保护; 炎症; 凋亡
Keywords:
ischemic stroke; Cerebral ischemia-reperfusion; Ophiopogonin D; Neuroprotection; Inflammation; Apoptosis
分类号:
R 743
DOI:
DOI:10.3969/j.issn.1000-7377.2026.01.006
文献标志码:
A
摘要:
目的：探讨麦冬皂苷D（OPD）单用或与依达拉奉（Edaravone）联用通过调控磷脂酰肌醇3激酶（PI3K）/蛋白激酶B（AKT）信号通路对脑缺血再灌注损伤（CIRI）的神经保护作用。方法：120只雌性C57BL/6小鼠，随机分为Sham组、脑缺血再灌注（MCAO/R）组、OPD组、Edaravone组和OPD+Edaravone组，每组24只。制备小鼠右侧大脑MCAO/R模型，脑缺血1 h后按分组方案给药，1次/d，持续7 d。每日称量体重，对小鼠进行神经行为学评分；转棒实验和旷场实验评估小鼠运动功能；激光散斑实验观察脑血流恢复情况；TTC染色观察脑梗死体积；HE染色、尼氏染色观察炎性细胞浸润以及神经元情况；WB检测缺血半暗带区相关蛋白表达。结果：造模后第5天和第7天，OPD、Edaravone及OPD+Edaravone组小鼠体重显著高于MCAO/R组（均P<0.01）。Zea-Longa神经评分显示，与MCAO/R组比较，三个治疗组第5天和第7天神经功能损伤明显减轻（均P<0.05）。与MCAO/R组比较，三个治疗组第3天和第7天停留时间显著延长，运动距离显著增加（均P<0.05）。OPD、Edaravone及OPD+Edaravone组小鼠在再灌注3 d和7 d的局部脑血流量（rCBF）比值显著增加（均P<0.05），脑梗死体积明显减少（P<0.001）。HE和Nissl染色显示，三个治疗组脑组织损伤减轻，炎性细胞浸润减少，细胞凋亡改善，尼氏小体减少减轻。WB结果表明，与MCAO/R组比较，三个治疗组Arg1、Bcl-xL、p-PI3K/PI3K和p-AKT/AKT蛋白表达显著升高，Iba1和iNOS蛋白表达显著下降（均P<0.05），同时OPD+Edaravone组Iba1和iNOS蛋白表达比较单独OPD组或单独Edaravone组显著降低（均P<0.05）。结论：麦冬皂苷D单用或与依达拉奉联用可显著缓解脑缺血再灌注损伤后的体重减轻、神经功能缺损、运动功能障碍，并通过激活PI3K/AKT信号通路改善炎症反应和抑制神经元凋亡。
Abstract:
Objective:To explore the neuroprotective effect of ophiopogonin D (OPD) alone or in combination with Edaravone on cerebral ischemia-reperfusion injury (CIRI) by regulating the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway.Methods：A total of 120 female C57BL/6 mice were randomly assigned to five groups (n=24 per group)：Sham group,cerebral ischemia-reperfusion (MCAO/R) group,OPD group,edaravone group,and OPD+edaravone group.Middle cerebral artery occlusion-reperfusion (MCAO/R) models were established in mice,followed by daily drug administration according to group assignments after 1 hour of cerebral ischemia,lasting for 7 days.Body weight and neurological behavior scores were recorded daily.Motor function was evaluated using rotarod and open field tests,cerebral blood flow recovery was monitored by laser speckle imaging,cerebral infarct volume was measured by TTC staining,and inflammatory cell infiltration and neuronal morphology were assessed via HE and Nissl staining.Western blotting was used to examine protein expression in the ischemic penumbra regions.Results:On day 5 and day 7 post-modeling,body weight was significantly higher in OPD,edaravone,and OPD+edaravone groups compared to the MCAO/R group (all P<0.01).Zea-Longa neurological scores showed that neurological deficits were significantly alleviated on day 5 and day 7 in all three treatment groups compared to the MCAO/R group (all P<0.05).The three treatment groups exhibited prolonged retention time and increased locomotion distance on day 3 and day 7 (all P<0.05).Regional cerebral blood flow (rCBF) ratios were significantly increased in OPD,edaravone,and OPD+edaravone groups at 3 and 7 days post-reperfusion (all P<0.05),accompanied by markedly reduced cerebral infarct volumes (P<0.001).HE and Nissl staining revealed attenuated brain tissue damage,reduced inflammatory cell infiltration,improved neuronal apoptosis,and preserved Nissl bodies in all treatment groups.Western blotting demonstrated that compared to the MCAO/R group,treatment groups showed upregulated Arg1 and Bcl-xL expression,increased ratios of p-PI3K/PI3K and p-AKT/AKT,and downregulated Iba1 and iNOS expression (all P<0.05).Notably,Iba1 and iNOS expression levels in the OPD+edaravone group were significantly lower than those in either the OPD or edaravone monotherapy groups (all P<0.05).Conclusion:Ophiopogonin D alone or combined with edaravone effectively alleviates weight loss,neurological deficits,and motor dysfunction following cerebral ischemia-reperfusion injury,exerting anti-inflammatory and anti-apoptotic effects through activation of the PI3K/AKT signaling pathway.

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备注/Memo

备注/Memo:
江西省中医药管理局科技计划项目（2022A309）
更新日期/Last Update: 2026-01-05