[1]赵云龙,赵宏达,任凯,等.丙泊酚调节MAPK/ERK信号通路对IL-1β诱导的软骨细胞损伤的改善作用实验研究[J].陕西医学杂志,2025,54(12):1622-1627.[doi:DOI:10.3969/j.issn.1000-7377.2025.12.006]
　ZHAO Yunlong,ZHAO Hongda,REN Kai,et al.Ameliorative effect of propofol on IL-1β-induced chondrocyte injury by regulating MAPK/ERK signaling pathway[J].,2025,54(12):1622-1627.[doi:DOI:10.3969/j.issn.1000-7377.2025.12.006]
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丙泊酚调节MAPK/ERK信号通路对IL-1β诱导的软骨细胞损伤的改善作用实验研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
54
期数:
2025年12期
页码:
1622-1627
栏目:
基础研究
出版日期:
2025-12-05

文章信息/Info

Title:
Ameliorative effect of propofol on IL-1β-induced chondrocyte injury by regulating MAPK/ERK signaling pathway
作者:
赵云龙1; 赵宏达2; 任凯3; 董丽强4
[1.哈尔滨市中医医院手术麻醉科，黑龙江 哈尔滨 150000；2.哈尔滨市第四医院麻醉科，黑龙江 哈尔滨 150026；3.哈尔滨市第一专科医院MECT（麻醉）科，黑龙江 哈尔滨 150000；4.黑龙江中医药大学附属第一医院手术麻醉科，黑龙江 哈尔滨 150040]
Author(s):
ZHAO Yunlong1; ZHAO Hongda2; REN Kai3; DONG Liqiang4
(1.Department of Surgical Anesthesiology,Harbin Hospital of Traditional Chinese Medicine,Harbin 150000,China;2.Department of Anesthesiology,Harbin Fourth Hospital,Harbin 150026,China;3.Department of MECT [Anesthesiology],Harbin First Specialized Hospital,Harbin 150000,China;4.Department of Surgical Anesthesiology,the First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China)
关键词:
软骨细胞损伤; 丙泊酚; 丝裂原活化蛋白激酶; 细胞外信号调节激酶; 白细胞介素-1β; 改善作用
Keywords:
Chondrocyte injury; Propofol; Mitogen-activated protein kinase; Extracellular regulated protein kinase; Interleukin-1β; Ameliorative effect
分类号:
R -33
DOI:
DOI:10.3969/j.issn.1000-7377.2025.12.006
文献标志码:
A
摘要:
目的：探讨丙泊酚（PRO）调节丝裂原活化蛋白激酶（MAPK）/细胞外信号调节激酶（ERK）信号通路对白细胞介素-1β（IL-1β）诱导的软骨细胞损伤的改善作用。方法：体外培养人软骨细胞C-28/I2，并随机分为Ctrl组、IL-1β组、PRO低剂量组（12.5 μmol/L PRO）、PRO中剂量组（25 μmol/L PRO）、PRO高剂量组（50 μmol/L PRO）和PRO高剂量+Chicoric acid组（50 μmol/L PRO+10 μmol/L MAPK/ERK信号通路激活剂Chicoric acid），其中对照组常规培养，其余各组均用10 ng/ml IL-1β诱导。CCK-8法检测C-28/I2细胞增殖；流式细胞术检测C-28/I2细胞凋亡；试剂盒检测C-28/I2细胞上清液中氧化应激指标；ELISA法检测C-28/I2细胞上清液中炎症因子水平；Western blot检测C-28/I2细胞中MAPK/ERK信号通路蛋白表达。结果：与IL-1β组比较，Ctrl组C-28/I2细胞存活率、细胞上清液中谷胱甘肽过氧化物酶（GSH-Px）水平降低，凋亡率、丙二醛（MDA）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）水平及p-p38 MAPK/p38 MAPK、p-ERK1/2/ERK1/2蛋白表达升高（均P＜0.05）。与IL-1β组比较，PRO低剂量组、PRO中剂量组和PRO高剂量组C-28/I2细胞存活率、细胞上清液中GSH-Px水平升高，凋亡率、MDA、IL-6、TNF-α水平及p-p38 MAPK/p38 MAPK、p-ERK1/2/ERK1/2蛋白表达降低，且呈剂量依赖性（均P＜0.05）。与PRO高剂量组比较，PRO高剂量+Chicoric acid组C-28/I2细胞存活率、细胞上清液中GSH-Px水平降低，凋亡率、MDA、IL-6、TNF-α水平及p-p38 MAPK/p38 MAPK、p-ERK1/2/ERK1/2蛋白表达升高（均P＜0.05）。结论：PRO可通过抑制MAPK/ERK信号通路改善IL-1β诱导的软骨细胞损伤。
Abstract:
Objective:To explore the ameliorative effect of propofol (PRO) on IL-1β-induced chondrocyte injury by regulating the MAPK/ERK signaling pathway.Methods：Human chondrocytes C-28/I2 were cultured in vitro and randomly classified into Ctrl group,IL-1β group,low-dose PRO group (12.5 μmol/L PRO),medium-dose PRO group (25 μmol/L PRO),high-dose PRO group (50 μmol/L PRO),and high-dose PRO+Chicolic acid group (50 μmol/L PRO+10 μmol/L MAPK/ERK signaling pathway activator Chicolic acid).The control group was cultured routinely,while the other groups were induced with 10 ng/ml IL-1β.CCK-8 method was used to measure the proliferation of C-28/I2 cells.Flow cytometry was performed to measure apoptosis in C-28/I2 cells.The reagent kit was used to measure oxidative stress indicators in the supernatant.ELISA method was used to detect the inflammatory factors in the supernatant.Western blot was performed to detect the MAPK/ERK signaling pathway proteins in C-28/I2 cells.Results:Compared with the Ctrl group,the IL-1β group showed a decrease in C-28/I2 cell survival rate,GSH-Px in cell supernatant,and an increase in apoptosis rate,MDA,IL-6,TNF-α levels,p-p38 MAPK/p38 MAPK and p-ERK1/2/ERK1/2 proteins (all P<0.05).Compared with the IL-1β group,the low-,medium- and high-dose PRO groups showed an increase in C-28/I2 cell survival rate,GSH-Px in cell supernatant,and a decrease in apoptosis rate,MDA,IL-6,TNF-α levels,p-p38 MAPK/p38 MAPK and p-ERK1/2/ERK1/2 proteins,and the changes were dose-dependent (all P<0.05).The high-dose PRO+Chicolic acid group showed a decrease in C-28/I2 cell survival rate,GSH-Px in cell supernatant,and an increase in apoptosis rate,MDA,IL-6,TNF-α levels,p-p38 MAPK/p38 MAPK and p-ERK1/2/ERK1/2 proteins than the high-dose PRO group (all P<0.05).Conclusion:PRO can ameliorate IL-1β-induced chondrocyte injury by inhibiting MAPK/ERK signaling pathway.

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备注/Memo

备注/Memo:
黑龙江省中医药科研项目（ZHY2024-080）
更新日期/Last Update: 2025-12-05