[1]罗红清,邓洁,徐丽,等.杜仲水提取物通过PI3K/AKT途径调节铁代谢改善糖尿病性骨质疏松症实验研究[J].陕西医学杂志,2025,54(12):1608-1615.[doi:DOI:10.3969/j.issn.1000-7377.2025.12.004]
　LUO Hongqing,DENG Jie,XU Li,et al.Eucommia ulmoides water extract regulates iron metabolism through PI3K/AKT pathway to improve diabetic osteoporosis[J].,2025,54(12):1608-1615.[doi:DOI:10.3969/j.issn.1000-7377.2025.12.004]
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杜仲水提取物通过PI3K/AKT途径调节铁代谢改善糖尿病性骨质疏松症实验研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
54
期数:
2025年12期
页码:
1608-1615
栏目:
基础研究
出版日期:
2025-12-05

文章信息/Info

Title:
Eucommia ulmoides water extract regulates iron metabolism through PI3K/AKT pathway to improve diabetic osteoporosis
作者:
罗红清1; 邓洁2; 徐丽3; 卢靖1; 李洪辉1; 邝涛1; 刘采群1
（1.湖南中医药大学第一附属医院脊柱二科，湖南 长沙 410007；2.长沙市妇幼保健院妇产科，湖南 长沙 410007；3.湖南中医药大学第一附属医院脊柱一科，湖南 长沙 410007）
Author(s):
LUO Hongqing1; DENG Jie2; XU Li3; LU Jing1; LI Honghui1; KUANG Tao1; LIU Caiqun1
(1.The Second Department of Spine,the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410007,China;2.Department of Obstetrics and Gynecology,Changsha Maternal and Child Health Hospital,Changsha 410007,China;3.The First Department of Spine,the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410007,China)
关键词:
糖尿病性骨质疏松症; 杜仲水提取物; 磷脂酰肌醇3-激酶; 蛋白激酶B; 信号通路; 铁代谢; 骨再生
Keywords:
Diabetic osteoporosis; Eucommia ulmoides water extract; Phosphatidylinositol 3-kinase; Protein kinase B; Signaling pathway; Iron metabolism; Bone regeneration
分类号:
R 285.5
DOI:
DOI:10.3969/j.issn.1000-7377.2025.12.004
文献标志码:
A
摘要:
目的：探讨杜仲（EUL）水提取物通过磷脂酰肌醇3-激酶（PI3K）/蛋白激酶B（AKT）通路调节铁代谢对糖尿病性骨质疏松症（DOP）中的改善作用。方法：将60只SD大鼠随机分为对照组、DOP组和EUL组，每组20只。通过高脂肪饮食结合链脲佐菌素注射建立DOP大鼠模型。EUL组大鼠口服EUL水提取物2.5 g/（kg·d），对照组和DOP组口服等量蒸馏水，持续12周。使用显微CT评估骨密度（BMD）、骨小梁数量（Tb.N）和骨小梁体积分数（BV/TV）。检测血清铁离子和丙二醛（MDA）水平，并通过Western blot分析PI3K/AKT信号通路相关蛋白的表达。使用MC3T3-E1成骨细胞模型分析EUL水提取物对细胞增殖、成骨分化及铁代谢的影响。结果：与对照组比较，DOP组大鼠BMD、Tb.N和BV/TV显著降低，EUL组大鼠BMD、Tb.N和BV/TV较DOP组显著增加（均P<0.05）。EUL处理显著降低了DOP大鼠血清铁离子和MDA水平，同时提高了骨组织中磷酸化PI3K（p-PI3K）和磷酸化AKT（p-AKT）蛋白的表达（均P<0.05）。在MC3T3-E1细胞中，EUL显著提高了高糖条件下的细胞增殖能力、ALP活性和钙沉积，并改善了细胞的铁代谢紊乱。加入PI3K抑制剂LY294002后，EUL的改善效果受到抑制。结论：EUL水提取物通过激活PI3K/AKT信号通路，可有效改善DOP模型中的铁代谢紊乱和成骨功能障碍。
Abstract:
Objective:To investigate the effect of Eucommia ulmoides (EUL) water extract on the improvement of diabetic osteoporosis (DOP) through regulating iron metabolism via the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) pathway.Methods：Sixty SD rats were randomly divided into the control group,the DOP group and the EUL group,with 20 rats in each group.The DOP rat model was established by using a high-fat diet combined with streptozotocin injection.The EUL group rats were orally administered 2.5 g/(kg�?偊bd) of EUL water extract,while the control group and the DOP group were orally administered the same amount of distilled water,for a duration of 12 weeks.Micro-CT was used to evaluate bone mineral density (BMD),trabecular number (Tb.N) and trabecular volume/total volume (BV/TV).Serum iron ions and malondialdehyde (MDA) levels were detected,and the expression of proteins related to the PI3K/AKT signaling pathway was analyzed by Western blot.The MC3T3-E1 osteoblast cell model was used to analyze the effects of EUL water extract on cell proliferation,osteogenic differentiation and iron metabolism.Results:Compared with the control group,the BMD,Tb.N and BV/TV in the DOP group were significantly decreased,while those in the EUL group were significantly increased compared with the DOP group (all P<0.05).EUL treatment significantly reduced the serum iron ions and MDA levels in DOP rats,and simultaneously increased the expression of p-PI3K and p-AKT proteins in bone tissue (all P<0.05).In MC3T3-E1 cells,EUL significantly enhanced the cell proliferation ability,ALP activity and calcium deposition under high glucose conditions,and improved the iron metabolism disorder of the cells.The improvement effect of EUL was inhibited after adding the PI3K inhibitor LY294002.Conclusion:The EUL water extract can effectively alleviate iron metabolism disorders and osteogenic dysfunction in the DOP model by activating the PI3K/AKT signaling pathway.

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备注/Memo

备注/Memo:
湖南省中医药科研课题（B2024042）
更新日期/Last Update: 2025-12-05