[1]张佳乐,李传坤.血清可溶性CD40配体、甘露聚糖结合凝集素相关丝氨酸蛋白酶3联合检测在老年急性缺血性脑卒中诊断中的价值[J].陕西医学杂志,2025,54(7):978-982.[doi:DOI:10.3969/j.issn.1000-7377.2025.07.021]
　ZHANG Jiale,LI Chuankun.The application value of serum sCD40L combined with MASP-3 in the diagnosis of acute ischemic stroke in the elderly[J].,2025,54(7):978-982.[doi:DOI:10.3969/j.issn.1000-7377.2025.07.021]
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血清可溶性CD40配体、甘露聚糖结合凝集素相关丝氨酸蛋白酶3联合检测在老年急性缺血性脑卒中诊断中的价值

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
54
期数:
2025年7期
页码:
978-982
栏目:
临床检验
出版日期:
2025-07-05

文章信息/Info

Title:
The application value of serum sCD40L combined with MASP-3 in the diagnosis of acute ischemic stroke in the elderly
作者:
张佳乐; 李传坤
（西安交通大学第一附属医院神经内科，陕西 西安 710061）
Author(s):
ZHANG Jiale; LI Chuankun
(Department of Neurosurgery，The First Affiliated Hospital of Xi’an Jiaotong University，Xi’an 710061，China）
关键词:
可溶性CD40配体; 甘露聚糖结合凝集素相关丝氨酸蛋白酶3; 急性缺血性脑卒中; 诊断; 血清; 老年人
Keywords:
Soluble CD40 ligand; Mannan binding lectin associated serine protease 3; Acute ischemic stroke; Diagnosis; Serum; Older people
分类号:
R 743
DOI:
DOI:10.3969/j.issn.1000-7377.2025.07.021
文献标志码:
A
摘要:
目的：探讨血清可溶性CD40配体（sCD40L）、甘露聚糖结合凝集素相关丝氨酸蛋白酶3（MASP-3）在老年急性缺血性脑卒中（AIS）的诊断价值。方法：选取82例老年AIS患者为AIS组，根据美国国立卫生院卒中量表（NIHSS）评分将其分为轻度组27例、中度组30例、重度组25例。选择老年体检健康志愿者82例为对照组，两组均于2022年1月至2024年3月收治。采用酶联免疫吸附试验（ELISA）检测血清中sCD40L、MASP-3水平；Spearman相关性分析AIS患者血清中sCD40L、MASP-3水平与NIHSS评分的关系；受试者工作特征（ROC）曲线分析诊断价值。Logistic回归分析影响因素。结果：AIS组较对照组的总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）水平显著升高，高密度脂蛋白胆固醇（HDL-C）水平显著降低（均P＜0.05）。与对照组比较，AIS患者血清中sCD40L水平显著升高，MASP-3水平显著降低（均P＜0.05）。血清sCD40L水平在AIS患者中与NIHSS评分呈正相关（r=0.691，P＜0.05），MASP-3水平则与之呈负相关（r=－0.409，P＜0.05）。与轻度组比较，中度组和重度组AIS患者血清中sCD40L水平、NIHSS评分显著升高，MASP-3水平显著降低（均P＜0.05）；与中度组比较，重度组sCD40L水平、NIHSS评分显著升高，MASP-3水平显著降低（均P＜0.05）；sCD40L、MASP-3联合诊断老年AIS的AUC高于sCD40L、MASP-3单独诊断的AUC值（Z=17.851，P＜0.001；Z=8.933，P＜0.001）。Logistic回归分析分析发现sCD40L（OR=2.364）、MASP-3（OR=0.639）、NIHSS评分（OR=4.258）水平是老年AIS发生的影响因素（均P＜0.05）。结论：老年AIS患者血清sCD40L高表达、MASP-3低表达，二者联合对老年AIS有较高诊断价值。
Abstract:
Objective:To investigate the diagnostic value of serum soluble CD40 ligand(sCD40L) combined with mannan-binding lectin-associated serine protease 3(MASP-3) in elderly patients with acute ischemic stroke(AIS).Methods：82 Elderly AIS patients were selected as AIS group，and were divided into mild group(27 cases)，moderate group(30 cases) and severe group(25 cases) according to the score of National Institutes of Health Stroke Scale(NIHSS).82 elderly healthy volunteers were selected as the control group，and both groups were treated from January 2022 to March 2024.Enzyme linked immunosorbent assay(ELISA) was applied to detect sCD40L and MASP-3 levels in serum；Spearman correlation was applied to analyze the relationship between serum sCD40L，MASP-3 levels and NIHSS score in AIS patients；diagnostic value of receiver operating characteristic(ROC) curve applysis.Logistic regression analysis was used to analyze the influencing factors.Results:Compared with the control group，the levels of total cholesterol(TC)，triglyceride(TG)，and low density lipoprotein cholesterol(LDL-C) in AIS group patients were obviously increased，while the level of high density lipoprotein cholesterol(HDL-C) was obviously reduced(all P＜0.05).Compared with the control group，the serum level of sCD40L in AIS patients was obviously increased，while the level of MASP-3 was obviously reduced(all P＜0.05).Serum sCD40L level in AIS patients was positively correlated with NIHSS score(r=0.691，P＜0.05)，while MASP-3 level was negatively correlated with the NIHSS score(r=－0.409，P＜0.05).Compared with the mild group，the serum sCD40L level and NIHSS score of AIS patients in the moderate and severe groups were obviously increased，while the MASP-3 level was obviously reduced(P＜0.05)；compared with the moderate group，the sCD40L level and NIHSS score in the severe group obviously increased，while the MASP-3 level obviously decreased(P＜0.05)；the AUC value of sCD40L combined with MASP-3 in diagnosis for elderly AIS was higher than that of sCD40L and MASP-3 alone diagnosis(Z=17.851，P＜0.001；Z=8.933，P＜0.001).Logistic regression analysis showed that sCD40L(OR=2.364)，MASP-3(OR=0.639) and NIHSS score(OR=4.258) were the influential factors for the occurrence of AIS in the elderly(P＜0.05).Conclusion:Elderly AIS patients have high expression of sCD40L and low expression of MASP-3 in their serum，the combination of the two has high diagnostic value for elderly AIS.

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备注/Memo

备注/Memo:
陕西省自然科学基础研究计划项目(2020JQ-509)
更新日期/Last Update: 2025-07-07