[1]刘铁兵,张石垒,刘飞,等.LINC01123调节miR-185-5p/TSPAN1轴对胰腺癌细胞增殖、迁移和侵袭的影响实验研究[J].陕西医学杂志,2025,54(6):736-741,747.[doi:DOI:10.3969/j.issn.1000-7377.2025.06.003]
　LIU Tiebing,ZHANG Shilei,LIU Fei,et al.Effects of LINC01123 on proliferation,migration and invasion of pancreatic cancer cells by regulating miR-185-5p/TSPAN1 axis[J].,2025,54(6):736-741,747.[doi:DOI:10.3969/j.issn.1000-7377.2025.06.003]
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LINC01123调节miR-185-5p/TSPAN1轴对胰腺癌细胞增殖、迁移和侵袭的影响实验研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
54
期数:
2025年6期
页码:
736-741,747
栏目:
基础研究
出版日期:
2025-06-05

文章信息/Info

Title:
Effects of LINC01123 on proliferation,migration and invasion of pancreatic cancer cells by regulating miR-185-5p/TSPAN1 axis
作者:
刘铁兵; 张石垒; 刘飞; 孙海宽; 郑雪迪; 刘江波
（河南科技大学临床医学院 河南科技大学第一附属医院乳腺肿瘤外科，河南 洛阳 471000）
Author(s):
LIU Tiebing; ZHANG Shilei; LIU Fei; SUN Haikuan; ZHENG Xuedi; LIU Jiangbo
(Department of Breast Tumor Surgery,School of Clinical Medicine,Henan University of Science and Technology,Luoyang 471000,China)
关键词:
胰腺癌; LINC01123; 微小RNA-185-5p; 跨膜蛋白1; 增殖; 迁移; 侵袭
Keywords:
Pancreatic cancer; LINC01123; miR-185-5p; Tetraspanin 1; Proliferation; Migration; Invasion
分类号:
R -33
DOI:
DOI:10.3969/j.issn.1000-7377.2025.06.003
文献标志码:
A
摘要:
目的：探讨LINC01123对胰腺癌（PC）细胞增殖、迁移和侵袭的影响及潜在分子机制。方法：收集45例PC患者的PC组织及邻近正常组织样本，体外培养人PC细胞系（PANC-1、SW1990、ASPC1、BxPC-3和CFPAC-1）。实时荧光定量PCR（qRT-PCR）检测组织和细胞LINC01123、微小RNA-185-5p（miR-185-5p）和跨膜蛋白1（TSPAN1）mRNA表达，分析PC组织中LINC01123、miR-185-5p、TSPAN1表达水平的相关性。将PANC-1细胞分为对照组、阴性对照组、LINC01123敲低组、抑制剂对照组和联合组，检测LINC01123、miR-185-5p及TSPAN1 mRNA和蛋白表达，以及细胞活力、细胞凋亡、侵袭和迁移；双荧光素酶报告基因分析PANC-1细胞中LINC01123、miR-185-5p和TSPAN1的调控作用。结果：与正常组织和细胞比较，PC组织和细胞中LINC01123和TSPAN1 mRNA表达升高，miR-185-5p表达降低，且miR-185-5p表达与LINC01123、TSPAN1 mRNA呈负相关，LINC01123表达与TSPAN1 mRNA呈正相关（均P<0.05）。沉默LINC01123可上调miR-185-5p表达，抑制TSPAN1表达，降低PANC-1细胞的增殖、迁移和侵袭能力，并促进细胞凋亡。抑制miR-185-5p可减弱LINC01123沉默对PANC-1细胞恶性生物学行为的抑制作用。双荧光素酶报告基因实验证实，LINC01123可靶向负调控miR-185-5p表达，TSPAN1是miR-185-5p的靶标。结论：沉默LINC01123可能通过miR-185-5p/TSPAN1轴抑制PC细胞恶性生物学行为。
Abstract:
Objective:To investigate the effects of LINC01123 on the proliferation,migration and invasion of pancreatic cancer (PC) cells and its potential molecular mechanism.Methods：PC tissue and adjacent normal tissue samples were collected from 45 PC patients,and human PC cell lines (PANC-1,SW1990,ASPC1,BxPC-3,and CFPAC-1) were cultured in vitro.RT-qPCR was used to detect the expression of LINC01123,miR-185-5p and tetraspanin 1 (TSPAN1) mRNA in tissues and cells.The correlation of LINC01123,miR-185-5p and TSPAN1 expression levels in PC tissues was analyzed.PANC-1 cells were assigned into control group,negative control group,LINC01123 knockdown group,inhibitor control group,and combined group.The expressions of LINC01123,miR-185-5p and TSPAN1 mRNA and protein,as well as cell viability,apoptosis,invasion and migration were detected.Dual luciferase reporter gene experiment was used to analyze the regulatory effects of LINC01123,miR-185-5p and TSPAN1 in PANC-1 cells.Results:Compared with normal tissues and cells,the expression levels of LINC01123 and TSPAN1 mRNA in PC tissues and cells were increased,while the expression level of miR-185-5p was decreased,and the expression of miR-185-5p was negatively correlated with LINC01123 and TSPAN1 mRNA,the expression of LINC01123 was positively correlated with TSPAN1 mRNA (all P<0.05).Silencing LINC01123 was able to upregulate miR-185-5p expression,inhibit TSPAN1 expression,reduce the proliferation,migration and invasion ability of PANC-1 cells,and promote cell apoptosis.Inhibition of miR-185-5p was able to weaken the inhibitory effect of LINC01123 silencing on the malignant biological behavior of PANC-1 cells.The dual luciferase reporter gene experiment confirmed that LINC01123 was able to target negative regulation of miR-185-5p expression,and TSPAN1 was the target of miR-185-5p.Conclusion:Silencing LINC01123 may inhibit the malignant biological behavior of PC cells through the miR-185-5p/TSPAN1 axis.

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备注/Memo

备注/Memo:
河南省医学科技攻关计划联合共建项目（LHGJ20210596）
更新日期/Last Update: 2025-06-04