[1]闫柳润,龚 婷,方峰杰.IL-37调节Nrf2/NF-κB信号通路对牙周炎大鼠炎症反应的影响[J].陕西医学杂志,2025,54(5):607-611.[doi:DOI:10.3969/j.issn.1000-7377.2025.05.006]
　YAN Liurun,GONG Ting,FANG Fengjie.Effect of IL-37 on inflammatory response in rats with periodontitis via regulation of the Nrf2/NF-κB signaling pathway[J].,2025,54(5):607-611.[doi:DOI:10.3969/j.issn.1000-7377.2025.05.006]
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IL-37调节Nrf2/NF-κB信号通路对牙周炎大鼠炎症反应的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
54
期数:
2025年5期
页码:
607-611
栏目:
基础研究
出版日期:
2025-05-05

文章信息/Info

Title:
Effect of IL-37 on inflammatory response in rats with periodontitis via regulation of the Nrf2/NF-κB signaling pathway
作者:
闫柳润; 龚 婷; 方峰杰
(安徽医科大学附属巢湖医院口腔科,安徽 合肥 238000)
Author(s):
YAN Liurun; GONG Ting; FANG Fengjie
(Department of Stomatology,Chaohu Hospital of Anhui Medical University,Hefei 238000,China)
关键词:
牙周炎;  白细胞介素-37;  核因子E2相关因子2;  核因子-κB;  炎症反应;  大鼠
Keywords:
Periodontitis;  Interleukin-37;  Nuclear factor erythroid 2-related factor 2;  Nuclear factor-κB;  Inflammatory response;  Rats
分类号:
R -33
DOI:
DOI:10.3969/j.issn.1000-7377.2025.05.006
文献标志码:
A
摘要:
目的:探讨白细胞介素-37(IL-37)对牙周炎大鼠炎症反应的影响,并初步探讨潜在的作用机制。方法:将30只大鼠随机分为对照组、模型组、IL-37组,每组10只。除对照组外,模型组和IL-37组建立大鼠牙周炎模型。术后每隔1日IL-37组于牙周袋内注射IL-37重组蛋白(1 μg),对照组和模型组同时牙周袋内注射等量0.9%氯化钠溶液。连续干预4周后,对各组大鼠牙龈指数(GI)、龈沟出血指数(SBI)、牙周附着丧失(AL)进行测定; 检测大鼠外周血炎症细胞(白细胞、淋巴细胞、巨噬细胞)数量; ELISA法检测大鼠牙周组织中促炎因子IL-6、IL-8和肿瘤坏死因子-α(TNF-α)水平,抗炎因子IL-37、IL-10表达水平; HE染色观察各组大鼠牙周组织病理学改变; Western blot检测各组大鼠牙周组织中细胞质和细胞核内核因子E2相关因子2(Nrf2)以及核因子-κB(NF-κB)蛋白磷酸化水平。结果:与对照组比较,模型组牙周组织发生明显病理损伤,牙周指标GI、SBI、AL升高; 与模型组比较,IL-37组牙周组织病理损伤明显改善,牙周指标GI、SBI、AL下降(均P<0.05)。与对照组比较,模型组外周血中炎症细胞数量及牙周组织中IL-6、IL-8和TNF-α水平升高,牙周组织中IL-37、IL-10水平降低; 与模型组比较,IL-37组外周血中炎症细胞数量及牙周组织中IL-6、IL-8和TNF-α水平降低,牙周组织中IL-37、IL-10水平升高(均P<0.05)。与对照组比较,模型组细胞核Nrf2蛋白表达水平降低,p-NF-κB p65及细胞质Nrf2蛋白表达水平升高; 与模型组比较,IL-37组细胞核Nrf2蛋白表达水平升高,p-NF-κB p65及细胞质Nrf2蛋白表达水平降低(均P<0.05)。结论:IL-37能减轻牙周炎大鼠牙周炎症反应,改善牙周组织损伤,其作用机制可能与调控Nrf2/NF-κB通路有关。
Abstract:
Objective:To investigate the effect of interleukin-37(IL-37)on inflammatory response in rats with periodontitis and preliminarily explore the underlying mechanisms.Methods:Thirty rats were randomly divided into three groups:control group,model group and IL-37 group,with 10 rats in each group.Except for the control group,periodontitis models were established in the model and IL-37 groups.The IL-37 group received intra-periodontal pocket injections of recombinant IL-37 protein(1 μg)every other day starting from the day after surgery,while the control and model groups received equivalent volumes of 0.9% sodium chloride solution.After 4 weeks of intervention,the gingival index(GI),sulcus bleeding index(SBI),and attachment loss(AL)were measured in each group.The number of peripheral blood inflammatory cells(white blood cells,lymphocytes and macrophages)was determined.Levels of pro-inflammatory cytokines(IL-6,IL-8 and TNF-α)and anti-inflammatory cytokines(IL-37,IL-10)in periodontal tissues were measured using ELISA.Histopathological changes in periodontal tissues were observed by HE staining.The levels of nuclear factor erythroid 2-related factor 2(Nrf2)in the cytoplasm and nucleus of periodontal tissues and the phosphorylation levels of nuclear factor-κB(NF-κB)protein were detected by Western blot.Results:Compared with the control group,the model group exhibited significant pathological damage in periodontal tissues and increased periodontal indices(GI,SBI and AL).Compared with the model group,the IL-37 group showed marked improvement in periodontal tissue damage and decreased periodontal indices(all P<0.05).Compared with the control group,the model group had increased numbers of peripheral blood inflammatory cells and elevated levels of IL-6,IL-8 and TNF-α in periodontal tissues,while IL-37 and IL-10 levels were decreased.In contrast,the IL-37 group had reduced numbers of peripheral blood inflammatory cells and lower levels of IL-6,IL-8 and TNF-α,with increased IL-37 and IL-10 levels in periodontal tissues(all P<0.05).Compared with the control group,the model group exhibited decreased nuclear Nrf2 protein expression and increased p-NF-κB p65 and cytoplasmic Nrf2 protein expression.Compared with the model group,the IL-37 group showed increased nuclear Nrf2 protein expression and decreased p-NF-κB p65 and cytoplasmic Nrf2 protein expression(all P<0.05).Conclusion:IL-37 can alleviate inflammatory response and improve periodontal tissue damage in rats with periodontitis.Its mechanism of action may be related to the regulation of the Nrf2/NF-κB signaling pathway.

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备注/Memo

备注/Memo:
[基金项目]安徽省卫生厅医药科研计划项目(11367KJ202101821)
更新日期/Last Update: 2025-05-05