[1]徐 律,张 炜,胡 志,等.长链非编码RNA LINC00106调控微小RNA-19b对膀胱癌细胞增殖和迁移的影响[J].陕西医学杂志,2025,54(5):579-583.[doi:DOI:10.3969/j.issn.1000-7377.2025.05.001]
　XU Lv,ZHANG Wei,HU Zhi,et al.Effects of long non-coding RNA LINC00106 on the proliferation and migration of bladder cancer cells via regulation of miR-19b[J].,2025,54(5):579-583.[doi:DOI:10.3969/j.issn.1000-7377.2025.05.001]
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长链非编码RNA LINC00106调控微小RNA-19b对膀胱癌细胞增殖和迁移的影响

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
54
期数:
2025年5期
页码:
579-583
栏目:
基础研究
出版日期:
2025-05-05

文章信息/Info

Title:
Effects of long non-coding RNA LINC00106 on the proliferation and migration of bladder cancer cells via regulation of miR-19b
作者:
徐 律1; 张 炜1; 胡 志1; 付 桥1; 孙 伟1; 盖强强2; 陈一衍1
(1.武汉大学附属同仁医院 武汉市第三医院泌尿外科,湖北 武汉 430074; 2.华中科技大学同济医学院附属同济医院泌尿外科,湖北 武汉 430030)
Author(s):
XU Lv1; ZHANG Wei1; HU Zhi1; FU Qiao1; SUN Wei1; GE Qiangqiang2; CHEN Yiyan1
(1.Department of Urology,Tongren Hospital of Wuhan University,Wuhan 430074,China; 2.Department of Urology,Tongji Hospital,Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430030,China)
关键词:
膀胱癌;  长链非编码RNA LINC00106;  微小RNA-19b;  细胞增殖;  细胞迁移
Keywords:
Bladder cancer;  Long non-coding RNA LINC00106;  miR-19b;  Cell proliferation;  Cell migration
分类号:
R 36
DOI:
DOI:10.3969/j.issn.1000-7377.2025.05.001
文献标志码:
A
摘要:
目的:探讨长链非编码RNA(lncRNA)LINC00106对膀胱癌细胞增殖和迁移的影响及其可能的机制。方法:Lnc2Cancer数据库分析LINC00106表达与膀胱癌患者总生存期和无病生存期的关系。实时荧光定量PCR(RT-qPCR)检测LINC00106在膀胱癌细胞系5637、J82、EJ、RT-4中的表达。体外培养膀胱癌5637细胞,并分别转染si-NC和si-LINC00106,分为si-NC组和si-LINC00106组。克隆形成实验和划痕实验分别检测干扰LINC00106表达对5637细胞增殖和迁移的影响。Western blot检测干扰LINC00106表达对5637细胞中香橼Rho相互作用激酶(CIT)、细胞周期蛋白依赖性激酶2(CDK2)、细胞周期蛋白E(Cyclin E)、Snail家族转录抑制因子2(SNAI2)、Twist相关蛋白1(Twist)蛋白表达的影响。LnCeVar靶基因在线软件预测LINC00106的下游靶基因。双荧光素酶报告基因实验验证LINC00106与微小RNA(miR)-19b的靶向关系。RT-qPCR检测干扰LINC00106表达对5637细胞中miR-19b表达的影响。结果:LINC00106低表达膀胱癌患者的总生存期和无病生存期均长于LINC00106高表达患者(均P<0.01)。LINC00106在膀胱癌细胞系5637、J82、EJ、RT-4中的表达量高于正常膀胱上皮SV-HUC-1细胞,且LINC00106在5637细胞中表达最高(均P<0.01)。干扰LINC00106表达后,5637细胞克隆形成数、划痕愈合率低于si-NC组(均P<0.01)。与si-NC组比较,si-LINC00106组5637细胞中CIT、CDK2、Cyclin E、SNAI2、Twist蛋白表达水平降低(均P<0.01)。LINC00106可靶向结合miR-19b。si-LINC00106组5637细胞中miR-19b表达水平高于si-NC组(P<0.01)。结论:LINC00106表达与膀胱癌患者生存期有关,干扰LINC00106表达通过靶向miR-19b抑制膀胱癌细胞的增殖和迁移。
Abstract:
Objective:To investigate the effects of long non-coding RNA(lncRNA)LINC00106 on the proliferation and migration of bladder cancer cells and its potential mechanisms.Methods:The relationship between LINC00106 expression and the overall survival and disease-free survival of bladder cancer patients was analyzed using the Lnc2Cancer database.The expression of LINC00106 in bladder cancer cell lines 5637,J82,EJ and RT-4 was detected by real-time quantitative PCR(RT-qPCR).Bladder cancer 5637 cells were cultured in vitro and transfected with si-NC or si-LINC00106,forming the si-NC group and si-LINC00106 group,respectively.Colony formation and scratch assays were used to assess the effects of LINC00106 knockdown on cell proliferation and migration in 5637 cells.Western blot was performed to detect the expression levels of Citron Rho-interacting kinase(CIT),cyclin-dependent kinase 2(CDK2),Cyclin E,Snail family transcriptional repressor 2(SNAI2),and Twist-related protein 1(Twist)in 5637 cells after LINC00106 knockdown.The downstream target genes of LINC00106 were predicted using the LnCeVar online software.The targeting relationship between LINC00106 and miR-19b was verified by dual-luciferase reporter assays.The expression of miR-19b in 5637 cells after LINC00106 knockdown was detected by RT-qPCR.Results:Patients with low LINC00106 expression had longer overall survival and disease-free survival compared to those with high LINC00106 expression(all P<0.01).LINC00106 expression was higher in bladder cancer cell lines 5637,J82,EJ and RT-4 than in normal bladder epithelial SV-HUC-1 cells,with the highest expression in 5637 cells(all P<0.01).After LINC00106 knockdown,the number of colonies and scratch wound healing rate in 5637 cells were significantly lower than those in the si-NC group(all P<0.01).Compared with the si-NC group,the expression levels of CIT,CDK2,Cyclin E,SNAI2 and Twist proteins in 5637 cells were decreased in the si-LINC00106 group(all P<0.01).LINC00106 was found to directly target miR-19b.The expression level of miR-19b in 5637 cells was higher in the si-LINC00106 group than in the si-NC group(P<0.01).Conclusion:LINC00106 expression is associated with the survival of bladder cancer patients.Knockdown of LINC00106 inhibits the proliferation and migration of bladder cancer cells by targeting miR-19b.

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备注/Memo

备注/Memo:
[基金项目]国家自然科学基金资助项目(82103284)
更新日期/Last Update: 2025-05-05