[1]李玉洁,韩 萌,杨国宁,等.芪参益气滴丸对慢性心衰大鼠线粒体分裂-融合及丝裂原活化蛋白激酶/细胞外信号调节激酶信号通路的影响[J].陕西医学杂志,2025,54(4):465-469,486.[doi:DOI:10.3969/j.issn.1000-7377.2025.04.006]
　LI Yujie,HAN Meng,YANG Guoning,et al.Effects of Qishenyiqi dropping pills on mitochondrial fission and fusion and MAPK/ERK1/2 signaling pathway in chronic heart failure rats[J].,2025,54(4):465-469,486.[doi:DOI:10.3969/j.issn.1000-7377.2025.04.006]
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芪参益气滴丸对慢性心衰大鼠线粒体分裂-融合及丝裂原活化蛋白激酶/细胞外信号调节激酶信号通路的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
54
期数:
2025年4期
页码:
465-469,486
栏目:
基础研究
出版日期:
2025-04-05

文章信息/Info

Title:
Effects of Qishenyiqi dropping pills on mitochondrial fission and fusion and MAPK/ERK1/2 signaling pathway in chronic heart failure rats
作者:
李玉洁1; 2; 韩 萌3; 杨国宁4; 胡 辉3; 林正捷3; 陈原林3
(1.邢台医学院附属医院心内科,河北 邢台 054000; 2.邢台市中心医院心内科,河北 邢台 054000; 3.邢台市中心医院静配中心,河北 邢台 054000; 4.邢台市中心医院心内科CCU,河北 邢台 054000)
Author(s):
LI Yujie1; 2; HAN Meng3; YANG Guoning4; HU Hui3; LIN Zhengjie3; CHEN Yuanlin3
(1.Department of Cardiology,Affiliated Hospital of Xingtai Medical College,Xingtai 054000,China; 2.Department of Cardiology,Xingtai Central Hospital,Xingtai 054000,China; 3.Static Distribution Center,Xingtai Central Hospital,Xingtai 054000,China; 4.CCU,Department of Cardiology,Xingtai Central Hospital,Xingtai 054000,China)
关键词:
慢性心力衰竭;  MAPK/ERK1/2信号通路;  芪参益气滴丸;  线粒体功能;  心肌细胞;  心功能
Keywords:
Chronic heart failure;  MAPK/ERK1/2 signaling pathway;  Qishenyiqi dropping pill;  Mitochondrial function;  Cardiomyocytes;  Cardiac function
分类号:
R 541.6
DOI:
DOI:10.3969/j.issn.1000-7377.2025.04.006
文献标志码:
A
摘要:
目的:探讨芪参益气滴丸对慢性心力衰竭(CHF)大鼠线粒体分裂-融合及丝裂原活化蛋白激酶/细胞外信号调节激酶(MAPK/ERK1/2)信号通路的影响。方法:选取60雄性SD大鼠,随机分为对照组、模型组、阳性对照组、芪参益气滴丸组,建立大鼠CHF模型,每组15只。对照组、模型组以10 ml/kg蒸馏水灌胃,每天1次。芪参益气滴丸组使用蒸馏水配制成300 kg/L药液灌胃,阳性对照组卡托普利组10 mg/kg灌胃,连续30 d。采用超声仪器测各组大鼠心功能,以苏木精-伊红(HE)染色观察心脏组织结构变化,JC-1染色法测细胞线粒体膜电位变化,蛋白质免疫印迹(Western blot)检测心脏组织中MAPK/ERK1/2通路及视神经萎缩蛋白1(OPA1)、动力相关蛋白1(Drp1)表达。结果:模型组与对照组比较,心功能下降,阳性对照组、芪参益气滴丸组与模型组比较心功能改善(均P<0.05)。模型组心肌细胞分布异常,形状和结构不完整,部分空泡化或已坏死,心肌纤维断裂,排列无序,较多炎性细胞浸润。而阳性对照组、芪参益气滴丸组心肌细胞形态趋于正常,个别细胞坏死,心肌纤维排列异常改善,仅少量炎性细胞浸润。模型组与对照组比较,绿色荧光增多,红色荧光减少,线粒体膜电位下降; 阳性对照组、芪参益气滴丸组与模型组比较,绿色荧光减少,红色荧光增多,线粒体膜电位升高(均P<0.05)。与对照组比较,模型组心肌组织OPA1蛋白表达下降,p-p38MAPK、p-ERK1/2、Drp1蛋白表达升高,与模型组比较,阳性对照组、芪参益气滴丸组心肌组织OPA1蛋白表达升高,p-p38MAPK、p-ERK1/2、Drp1蛋白表达下降(均P<0.05)。结论:芪参益气滴丸能够改善线粒体功能,发挥心肌保护作用,其机制可能与MAPK/ERK1/2通路有关。
Abstract:
Objective:To investigate the effects of Qishenyiqi dropping pills on mitogen-activated protein kinase/extracellular signal-regulated kinase(MAPK/ERK1/2)signaling pathway in chronic heart failure(CHF)rats.Methods:Sixty male SD rats were randomly divided into control group,model group,positive control group and Qishenyiqi dropping pill group.CHF model of rats was established,there were 15 rats in each group.Control group and model group were given 10 ml/kg distilled water once a day.Qishenyiqi dropping pill group was given 300 kg /L liquid prepared with distilled water by intragastric administration,while captopril group was given 10 mg/kg intragastric administration for 30 days.Using ultrasonic instruments measured between groups in the rat cardiac function,with hematoxylin-eosin(HE)staining to observe heart organization structure change,JC-1 staining cells mitochondrial membrane potential changes,Western Blot was used to detect the MAPK/ERK1/2 pathway and the expression of optic atrophy protein 1(OPA1)and dynamin-related protein 1(Drp1)in heart tissue.Results:Compared with the control group,the heart function of the model group decreased,and the positive control group and Qishenyiqi dropping pill group improved the heart function compared with the model group(P<0.05).Myocardial cells in the model group were abnormal in distribution,incomplete in shape and structure,partially vacuolated or necrotic,broken myocardial fibers,disordered,and infiltrated by more inflammatory cells.The morphology and morphology of cardiomyocytes in positive control group and Qishenyiqi dropping pill group tended to be normal,some cells were necrotic,the arrangement of myocardial fibers was abnormally improved,and only a few inflammatory cells infiltrated.Compared with the model group,the green fluorescence increased,the red fluorescence decreased,and the mitochondrial membrane potential decreased; compared with the model group,the positive control group and Qishenyiqi dropping pill group reduced the green fluorescence,increased the red fluorescence,and the mitochondrial membrane potential increased(all P<0.05).Compared with the control group,the expression of OPA1 protein in cardiac tissue of the model group decreased,while the expression of p-p38MAPK,p-ERK1/2 and Drp1 protein increased.Compared with the model group,the expression of OPA1 protein in cardiac tissue of the positive control group and Qishenyiqi dropping pill group increased.The protein expressions of P-P38MAPK,P-ERK1/2 and Drp1 were decreased(all P<0.05).Conclusion:Qishenyiqi dropping pills can improve mitochondrial function and exert myocardial protection,the mechanism of which may be related to MAPK/ERK1/2 pathway.

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备注/Memo

备注/Memo:
[基金项目]河北省中医药管理局科研计划项目(2021453); 河北省邢台市科技计划项目(2023ZC067)
更新日期/Last Update: 2025-04-07