[1]王俊诸,郭 威,范 曦,等.黄芪多糖联合维生素C对镉致雌性大鼠肾脏损伤的保护作用[J].陕西医学杂志,2025,54(4):458-464.[doi:DOI:10.3969/j.issn.1000-7377.2025.04.005]
　WANG Junzhu,GUO Wei,FAN Xi,et al.Protective effect of Astragalus polysaccharide combined with vitamin C on cadmium induced kidney injury in female rats[J].,2025,54(4):458-464.[doi:DOI:10.3969/j.issn.1000-7377.2025.04.005]
点击复制

黄芪多糖联合维生素C对镉致雌性大鼠肾脏损伤的保护作用
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
54
期数:
2025年4期
页码:
458-464
栏目:
基础研究
出版日期:
2025-04-05

文章信息/Info

Title:
Protective effect of Astragalus polysaccharide combined with vitamin C on cadmium induced kidney injury in female rats
作者:
王俊诸1; 郭 威1; 范 曦1; 黄 强1; 杨 俊2; 陈 蓉1
(1.长沙医学院,湖南 长沙 410219; 2.湖南中医药大学第一附属医院,湖南 长沙 410007)
Author(s):
WANG Junzhu1; GUO Wei1; FAN Xi1; HUANG Qiang1; YANG Jun2; CHEN Rong1
(1.Changsha Medical University,Changsha 410219,China; 2.The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410007,China)
关键词:
镉;  黄芪多糖;  维生素C;  肾脏;  凋亡;  半胱氨酸天冬氨酸蛋白酶-3
Keywords:
Cadmium;  Astragalus polysaccharides;  Vitamin C;  Kidney;  Apoptosis;  Casepase-3
分类号:
R692
DOI:
DOI:10.3969/j.issn.1000-7377.2025.04.005
文献标志码:
A
摘要:
目的:探究黄芪多糖联合维生素C对镉致雌性大鼠肾脏损伤的保护作用。方法:将24只SD雌性大鼠随机分成四组每组6只,分别为正常对照组、镉染毒组、黄芪多糖联合维生素C低浓度组、黄芪多糖联合维生素C高浓度组。试验结束后处死大鼠采集新鲜血液,生物化学法检测大鼠肾功能生化指标肌酐(CRE)含量、尿素氮(BUN)含量,并采集其肾脏组织进行苏木精-伊红染色法(HE)观察肾组织病理变化情况; 马松染色法(Masson)观察肾组织纤维化情况; 免疫荧光法(IF)、免疫组化法(IHC)检测肾组织中半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)蛋白表达情况; 原位末端标记法(TUNEL荧光染色)检测肾组织细胞凋亡情况。结果:经氯化镉长期腹腔注射后,镉染毒组雌性大鼠生化结果显示血清中CRE、BUN含量显著升高; HE结果显示可见少量肾小管上皮细胞水肿,细胞肿胀,胞质疏松淡染,间质明显增生,可见少量炎性细胞浸润,肾小体形态发生不规则改变,肾小囊与肾小球之间界限模糊,球囊粘连; Masson结果显示肾脏组织可见大量蓝色胶原纤维; IF、IHC结果均显示凋亡信号相关通路Caspase-3的表达显著升高; TUNEL荧光染色结果显示肾脏组织细胞TUENL阳性表达率显著升高,肾脏组织细胞出现大量凋亡。较镉染毒组比较黄芪多糖联合维生素C低、高浓度组血清中CRE、BUN含量、凋亡信号相关通路Caspase-3的表达显著降低; 病理损伤、肾脏组织纤维化、肾脏组织细胞凋亡等方面均出现不同程度的改善。结论:黄芪多糖联合维生素C对镉致雌性大鼠肾脏组织的损伤具有保护作用,可有效拮抗镉对肾脏组织的损伤,减少肾脏组织细胞的凋亡并恢复肾脏功能。
Abstract:
Objective:To investigate the protective effect of Astragalus polysaccharides combined with vitamin C on cadmium induced kidney injury in female rats.Method:24 female SD rats were randomly divided into 4 groups,with 6 rats in each group:normal control group,cadmium exposure group,low concentration group of Huangqi polysaccharide combined with vitamin C,and high concentration group of Huangqi polysaccharide combined with vitamin C.After the experiment,the rats were euthanized and fresh blood was collected.Biochemical methods were used to detect the biochemical indicators of creatinine(CRE)and urea nitrogen(BUN)in the kidney function of the rats,and their kidney tissue was collected for hematoxylin and eosin(HE)staining to observe the pathological changes in the kidney tissue; Observation of renal tissue fibrosis using Masson staining method(Masson); Immunofluorescence assay(IF),immunohistochemistry(IHC),and detection of Caspase-3 protein expression in renal tissue; Detection of renal tissue cell apoptosis using in situ end labeling(TUNEL fluorescence staining)method.Results:After long-term intraperitoneal injection of cadmium chloride,the biochemical results of female rats in the cadmium exposed group showed a significant increase in serum CRE and BUN levels; The H&E results showed that a small amount of renal tubular epithelial cells were edematous,swollen,with loose and lightly stained cytoplasm and significant interstitial proliferation.A small number of inflammatory cell infiltration was observed,and irregular changes in renal corpuscle morphology were observed.The boundary between the renal capsule and glomerulus was blurred,and balloon adhesions were observed; the Masson results showed a large number of blue collagen fibers in the kidney tissue; Both IF and IHC results showed a significant increase in the expression of the apoptotic signaling pathway Caspase-3; the TUNEL fluorescence staining results showed a significant increase in the positive expression rate of TUNEL in renal tissue cells,and a large amount of apoptosis occurred in renal tissue cells.Compared with the cadmium exposure group,the serum creatinine content,urea nitrogen content,and expression of apoptosis signal related pathway Caspase-3 were significantly reduced in the low and high-dose groups of Astragalus polysaccharide combined with vitamin C; Pathological damage,renal tissue fibrosis,and renal tissue cell apoptosis have all shown varying degrees of improvement.Conclusion:Astragalus polysaccharide combined with vitamin C has a protective effect on cadmium induced kidney tissue damage in female rats,effectively antagonizing cadmium induced kidney tissue damage,reducing apoptosis of kidney tissue cells,and restoring kidney function.

参考文献/References:

[1] 王妍.我国有色金属工业土壤重金属污染防治的现状与对策[J].有色金属(冶炼部分),2021(3):1-9.
[2] WU H,LIAO Q,CHILLRUD S N,et al.Environmental ex-posure to cadmium:health risk assessment and its as- sociations with hypertension and impaired kidney function[J].Sci Rep,2016,6:29989.
[3] 李敏,杨前,刘亚轩,等.镉通过诱导自噬促进肾小管上皮细胞铁死亡的研究[J].陆军军医大学学报,2022,44(17):1705-1711.
[4] THÉVENOD F.Catch me if you can Novel aspects of cadmium transport in mammalian cells[J].Biometals,2010,23(5):857-875.
[5] LIN S,ZHU H,QIN H Z,et al.Effect of Stemona tuberosa alkaloids on apoptosis of SMMC-7721cells and expression of Bcl-2,Bax,and cleaved caspase-3[J].Chin J Exp Tradit Med Form,2021,27(19):73-79.
[6] 龚频,白晓换,陈福欣,等.镉损伤小鼠肾脏形态学研究及姜黄素的保护作用[J].陕西科技大学学报:自然科学版,2015,35(3):140-143.
[7] PARI L,SHAGIRTHA K.Hesperetin protects against oxidative stress related hepatic dysfunction by cadmium in rats[J].Exp Toxicol Pathol,2012,64(5):513-520.
[8] PRABU S M,SHAGIRTHA K,RENUGADEVI J.Quercetin in combination with vitamins(C and E)improves oxidative stress and renal injury in cadmium intoxicated rats[J].Eur Rev Med Pharmacol Sci,2010,14(11):903-914.
[9] 王珍,尤其嘉,杨靖亚,等.芦根多糖对镉中毒小鼠肝肾组织损伤的保护作用[J].食品工业科技,2013,34(2):349-352.
[10] 郑小蓉.黄芪多糖对镉染毒大鼠肝脏损伤的保护作用[J].工业卫生与职业病,2018,44(6):418-420,426.
[11] 安方玉,颜春鲁,刘永琦,等.黄芪多糖对镉染毒大鼠肾损伤的保护作用[J].中国临床药理学杂志,2018,34(1):49-52.
[12] PALLER M S,HOIDAL J R,FERRIS T F.Oxygen free radicals in ischemic acute renal failure in the rat[J].J Clin Invest,1984,4(4):1156-1164.
[13] YIMING Z,HANG Y,BING S,et al.Antagonistic effect of VDR/CREB1 pathway on cadmium-induced apoptosis in porcine spleen[J].Ecotoxicol Environ Saf,2021,209:111819.
[14] 李建华,李宁侠,陶绍辉,等.未成年人2552例体内镉、铜、铁、铅、锌、钙、镁元素水平调查研究[J].陕西医学杂志,2020,49(12):1691-1694.
[15] 许慧,王菊玉,张立梅,等.槲皮素对小鼠肝肾慢性镉中毒的保护作用[J].中国兽医杂志,2023,59(4):137-142.
[16] RANI A,KUMAR A,LAL A,et al.Cellular mechanisms of cadmium-induced toxicity:A review[J].Int J En-viron Health Res,2014,24(4):378-399.
[17] KIM K S,LIM H J,LIM J S,et al.Curcumin amelio-rates cadmium-induced nephrotoxicity in sprague dawley rats[J].Food Chem Toxicol,2018,114:34-40.
[18] JOHRI N,JACQUILLET G,UNWIN R.Heavy metal poisoning:The effects of cadmium on the kidney[J].Bio-metals,2010,23(5):783-792.
[19] 谭汉全,尹雪梅.PTH、BUN及Cr联合检测在肾功能损伤早期诊断中的临床价值[J].海南医学院学报,2015,21(2):197-199.
[20] 丁小丽,董文轩,宫中桂,等.长期镉暴露对大鼠肾纤维化的影响及葛根素的保护效应[J].甘肃农业大学学报,2023,58(4):1-8,20.
[21] 李有幸,陆钦晨,庞雅琴,等.亚慢性镉暴露对大鼠血常规和肝肾组织的影响[J].右江民族医学院学报,2021,43(2):155-159.
[22] 丁通,骆骄阳,杨世海,等.天然药物防治镉中毒的现代研究进展[J].中国中药杂志,2018,43(10):2006-2013.
[23] 邢喜平,何玉林,薛娜,等.黄芪多糖对镉染毒大鼠肝肾损伤改善和肠道菌群结构调节的作用[J].中国药理学通报,2023,39(2):332-339.
[24] 李红梅.标准物质质量控制及不确定度评定[M].北京:中国标准出版社,2014:126-128.

备注/Memo

备注/Memo:
[基金项目]国家级大学生创新训练计划项目(教高司函[2023]8号-S202310823014); 湖南省大学生创新创业训练计划项目(湘教通[2023]237号-4016); 湖南省教育厅科学研究项目(20C0179)
更新日期/Last Update: 2025-04-07