[1]张静妮,张 莹,岳 虹.自身免疫性肝炎患者肝组织与外周血T细胞免疫球蛋白及黏蛋白结构域分子3、细胞因子信号转导抑制因子3表达及临床意义[J].陕西医学杂志,2025,54(3):404-407,423.[doi:DOI:10.3969/j.issn.1000-7377.2025.03.023]
　ZHANG Jingni,ZHANG Ying,YUE Hong.Expression and clinical significance of Tim-3 and SOCS3 in liver tissue and peripheral blood of patients with autoimmune hepatitis[J].,2025,54(3):404-407,423.[doi:DOI:10.3969/j.issn.1000-7377.2025.03.023]
点击复制

自身免疫性肝炎患者肝组织与外周血T细胞免疫球蛋白及黏蛋白结构域分子3、细胞因子信号转导抑制因子3表达及临床意义
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
54
期数:
2025年3期
页码:
404-407,423
栏目:
临床病理
出版日期:
2025-03-05

文章信息/Info

Title:
Expression and clinical significance of Tim-3 and SOCS3 in liver tissue and peripheral blood of patients with autoimmune hepatitis
作者:
张静妮1; 张 莹2; 岳 虹2
(1.西安市红会医院检验科,陕西 西安 710054; 2.西安市第一医院检验科,陕西 西安 710002)
Author(s):
ZHANG Jingni1; ZHANG Ying2; YUE Hong2
(1.Department of Laboratory,Xi'an Honghui Hospital,Xi'an 710054,China; 2.Department of Laboratory,Xi'an No.1 Hospital,Xi'an 710002,China)
关键词:
自身免疫性肝炎;  T细胞免疫球蛋白及黏蛋白结构域分子3;  细胞因子信号转导抑制因子3;  炎症因子;  趋化因子
Keywords:
Autoimmune hepatitis;  T cell immunoglobulin and mucin domain containing molecule 3;  Suppressor of cytokine signaling 3;  Inflammatory factors;  Chemokines
分类号:
R 575.1
DOI:
DOI:10.3969/j.issn.1000-7377.2025.03.023
文献标志码:
A
摘要:
目的:探究自身免疫性肝炎患者肝组织与外周血T细胞免疫球蛋白及黏蛋白结构域分子3(Tim-3)、细胞因子信号转导抑制因子3(SOCS3)表达及诊断价值。方法:选取自身免疫性肝炎患者83例为自身免疫性肝炎组,选取体检健康者86例为对照组。采用免疫组化染色和酶联免疫吸附法检测肝组织及外周血Tim-3、SOCS3表达。采用全自动生化分析仪检测自身免疫相关的炎症因子白细胞介素-2(IL-2)、IL-6、IL-21以及趋化因子C-C Motif趋化因子配体2(CCL2)、C-X-C Motif趋化因子配体9(CXCL9)、趋化因子C-C-基元受体6(CCR6)表达。采用Pearson相关性分析Tim-3、SOCS3表达与炎症因子和趋化因子的相关性。采用受试者工作特征(ROC)曲线分析Tim-3、SOCS3表达水平对自身免疫性肝炎的诊断价值。结果:免疫组化染色结果显示,自身免疫性肝炎组Tim-3、SOCS3阳性表达率高于对照组(均P<0.05)。与对照组比较,自身免疫性肝炎组炎症因子(IL-2、IL-6、IL-21)、趋化因子(CCL2、CXCL9、CCR6)以及Tim-3和SOCS3水平显著升高(均P<0.05)。Pearson相关性分析显示,Tim-3、SOCS3与炎症因子IL-2、IL-6、IL-21及趋化因子CCL2、CXCL9、CCR6呈正相关(均P<0.05)。ROC曲线分析显示,Tim-3、SOCS3联合检测对自身免疫性肝炎的诊断价值优于各自单独检测(均P<0.05)。结论:Tim-3、SOCS3在自身免疫性肝炎患者肝组织及外周血中均呈高表达,两者联合检测对自身免疫性肝炎具有良好的诊断价值。
Abstract:
Objective:To investigate the expression and clinical significance of T cell immunoglobulin and mucin domain containing molecule 3(Tim-3)and suppressor of cytokine signaling 3(SOCS3)in liver tissue and peripheral blood of patients with autoimmune hepatitis.Methods:Eighty-three patients with autoimmune hepatitis were selected as autoimmune hepatitis group,and 86 healthy people were selected as control group.The expressions of Tim-3 and SOCS3 in peripheral blood and liver tissues were detected by immunohistochemical staining and enzyme-linked immunosorbent assay.Autoimmune-related inflammatory factors IL-2,IL-6,IL-21,CCL2,CXCL9 CCR6 were detected by automatic biochemical analyzer.Pearson correlation analysis was used to analyze the correlation between the expressions of Tim-3,SOCS3 and inflammatory factors and chemokines.ROC curve was used to analyze the diagnostic value of Tim-3 and SOCS3 expression levels for autoimmune hepatitis.Results:Immunohistochemical staining showed that the positive expression rates of Tim-3 and SOCS3 in the autoimmune hepatitis group were higher than those in the control group(both P<0.05).Compared with the control group,the levels of inflammatory factors(IL-2,IL-6,IL-21),chemokines(CCL2,CXCL9,CCR6),Tim-3 and SOCS3 in the autoimmune hepatitis group were significantly increased(all P<0.05).Pearson correlation analysis showed that Tim-3 and SOCS3 were positively correlated with inflammatory factors IL-2,IL-6,IL-21 and chemokines CCL2,CXCL9,CCR6(all P<0.05).ROC curve analysis showed that the diagnostic value of Tim-3 and SOCS3 combined detection for autoimmune hepatitis was better than that of each single detection(all P<0.05).Conclusion:Tim-3 and SOCS3 are highly expressed in liver tissue and peripheral blood of patients with autoimmune hepatitis,and the combined detection of TIM-3 and SOCS3 has a good diagnostic value for autoimmune hepatitis.

参考文献/References:

[1] 李兵.自身免疫性肝炎诊断中肝病相关抗体与生化检测指标的意义分析[J].中国现代药物应用,2023,17(9):83-85.
[2] 孙小媚,刘微,阚丽娜,等.自身免疫性肝炎、原发性胆汁性肝硬化及其重叠综合征患者白介素-17、核转录因子-κB、转化生长因子β1表达水平及意义[J].陕西医学杂志,2023,52(10):1367-1370.
[3] 牛斌.CCL20、CCR6、IL-21血清水平变化与自身免疫性肝炎患者肝组织炎症活动分级的关联性分析[J].医学检验与临床,2021,32(10):8-15.
[4] BERETTA-PICCOLI B T,MIELI-VERGANIG,VERGANI D.Autoimmune hepatitis:Serum autoantibodies in clinical practice[J].Clin Rev Allergy Immunol,2022,63(2):124-137.
[5] YAN W,LIU X,MA H,et al.Tim-3 fosters HCC development by enhancing TGF-β-mediated alternative activation of macrophages[J].Gut,2015,64(10):1593-1604.
[6] 戴雯,魏艳艳,张柳.Tim-3靶向CD8+ T细胞调控TNF-α/IL-22/STAT3通路加重自身免疫性肝炎小鼠肝损伤[J].山西医科大学学报,2023,54(3):327-332.
[7] 刘娜,张华敏,邓巧娟,等.SOCS3过表达对自身免疫性肝炎小鼠的保护作用[J].现代消化及介入诊疗,2020,25(7):899-906.
[8] 中华医学会肝病学分会,中华医学会消化病学分会,中华医学会感染病学分会.自身免疫性肝炎诊断和治疗共识(2015)[J].临床肝胆病杂志,2016,32(1):9-22.
[9] 凌晶,张友健,宋雄峰.自身免疫性肝炎患者血清趋化因子和GP73水平变化及其临床意义研究[J].实用肝脏病杂志,2020,23(6):825-828.
[10] 齐红梅.老年自身免疫性肝炎的临床检验特点分析[J].智慧健康,2020,6(32):18-30.
[11] 冯静,罗思剑,李靖,等.不同类型自身免疫性肝病患者肝组织炎症因子表达的变化[J].实用肝脏病杂志,2020,23(3):356-359.
[12] MROSKOWIAK A,SULEJA A,STEC M,et al.Autoimmune hepatitis-challenging diagnosis[J].Medicina(Kaunas),2022,58(7):896-905.
[13] ZHAO L,YU G,HAN Q,et al.TIM-3:An emerging target in the liver diseases[J].Scand J Immunol,2020,91(4):128-135.
[14] 张娟梓,赵远瑜,韩江.Tim-3在脑梗死后胶质细胞和巨噬细胞中作用的研究进展[J].中风与神经疾病杂志,2022,39(2):178-180.
[15] 孟祖东,杜天平,王玉.小柴胡汤治疗系统性红斑狼疮临床疗效及对血清TIM-1、TIM-3水平的影响[J].陕西中医,2017,38(11):1544-1545.
[16] WU H,TANG S,ZHOU M,et al.Tim-3 suppresses autoimmune hepatitis via the p38/MKP-1 pathway in Th17 cells[J].FEBS Open Bio,2021,1(5):1406-1416.
[17] 刘琼,杨歆,唐简,等.慢性乙型肝炎患者外周血单个核细胞Tim-3和血清IL-33表达及临床意义[J].中华医院感染学杂志,2020,30(13):2014-2017.
[18] MATSUMURA S,NAKAMORI M,TSUJI T,et al.Oncolytic virotherapy with SOCS3 enhances viral replicative potency and oncolysis for gastric cancer[J].Oncotarget,2021,12(4):344-354.
[19] 李欣泽,王彦君,李杨,等.镰形棘豆总黄酮通过调控JAK1/STAT1/SOCS3信号通路减轻特发性肺纤维化[J].中国病理生理杂志,2022,38(5):905-912.
[20] 李扬,徐骏驰,陈帆,等.细胞因子信号转导抑制因子3在慢加急性肝衰竭小鼠的表达[J].江苏医药,2023,49(4):325-329.
[21] 赵寅洲,张茹薏,赵家洁,等.慢性乙型肝炎患者外周血SOCS3 mRNA的表达及其与Th17的关系[J].中国感染控制杂志,2022,21(9):870-876.
[22] 赵家洁,游晶,李静,等.SOCS的免疫调节机制及其在慢性肝病中的作用[J].中国免疫学杂志,2022,38(17):2140-2146.
[23] 吴昱,李青青,王应,等.PSMP/MSMP蛋白通过C-C基序趋化因子受体2途径促进肝纤维化[J].临床肝胆病杂志,2020,36(4):903-908.
[24] 汪道伟,方坚,范凌.自身免疫性肝炎患者血清IL-21和趋化因子水平变化及其临床意义探讨[J].实用肝脏病杂志,2019,22(6):844-847.
[25] 艾国,雒真龙,张志成,等.炎症相关细胞因子及趋化因子在小鼠自身免疫性肝损伤不同时期的表达及作用[J].医学研究杂志,2020,49(2):20-24.

相似文献/References:

[1]孙小媚,刘 微,阚丽娜,等.自身免疫性肝炎、原发性胆汁性肝硬化及其重叠综合征患者白介素-17、核转录因子-κB、转化生长因子β1表达水平及意义[J].陕西医学杂志,2023,52(10):1367.[doi:DOI:10.3969/j.issn.1000-7377.2023.10.018]

备注/Memo

备注/Memo:
[基金项目]陕西省重点研发计划项目(2019SF-116)
更新日期/Last Update: 2025-03-05