[1]秦 杰,张一娇,路晓琳,等.基于液基细胞学的hWAPL蛋白检验在高危型人乳头瘤病毒感染患者宫颈癌筛查中的价值研究[J].陕西医学杂志,2025,54(2):268-271,276.[doi:DOI:10.3969/j.issn.1000-7377.2025.02.026]
　QIN Jie,ZHANG Yijiao,LU Xiaolin,et al.Value of hWAPL protein test based on liquid-based cytology in cervical cancer screening in high-risk human papillomavirus infected patients[J].,2025,54(2):268-271,276.[doi:DOI:10.3969/j.issn.1000-7377.2025.02.026]
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基于液基细胞学的hWAPL蛋白检验在高危型人乳头瘤病毒感染患者宫颈癌筛查中的价值研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
54
期数:
2025年2期
页码:
268-271,276
栏目:
临床病理
出版日期:
2025-02-05

文章信息/Info

Title:
Value of hWAPL protein test based on liquid-based cytology in cervical cancer screening in high-risk human papillomavirus infected patients
作者:
秦 杰; 张一娇; 路晓琳; 董有伟; 段爱红
(邯郸市中心医院,河北 邯郸 056001)
Author(s):
QIN Jie; ZHANG Yijiao; LU Xiaolin; DONG Youwei; DUAN Aihong
(Handan Central Hospital,Handan 056001,China)
关键词:
宫颈癌;  人半翼蛋白;  人乳头瘤病毒;  液基细胞学;  组织病理学;  筛查效能
Keywords:
Cervical cancer;  Human wings apart-like protein;  Human papillomavirus;  Liquid-based cytology;  Histopathology;  Screening efficacy
分类号:
R 737.33
DOI:
DOI:10.3969/j.issn.1000-7377.2025.02.026
文献标志码:
A
摘要:
目的:探讨基于液基细胞学的人半翼(hWAPL)蛋白检验在高危型人乳头瘤病毒(HR-HPV)感染患者宫颈癌筛查中的价值。方法:选取300例HPV感染女性为研究对象。采用杂交捕获技术进行常规HPV-DNA检测,阴道镜下取宫颈组织标本行组织病理学检查,并进行液基细胞学检查。采用免疫细胞化学技术检测液基细胞hWAPL蛋白表达。采用Kappa检验分析单独液基细胞学检查、液基细胞hWAPL蛋白检测与组织病理学检查结果的一致性,并比较单独液基细胞学检查和液基细胞hWAPL蛋白检测对HR-HPV感染患者宫颈癌的筛查效能。结果:300例宫颈液基细胞学标本中,共检测到18种HR-HPV亚型和5种低危型HPV亚型,其中HR-HPV感染患者236例,感染率为78.67%。组织病理学检查结果显示,阳性64例,阳性率为27.12%。单独液基细胞学检查结果显示,50例患者为阳性,阳性率为21.19%,与组织病理学检查一致性较高(Kappa=0.655,P<0.05)。液基细胞hWAPL蛋白检测结果显示,58例患者为阳性,阳性率为24.58%,与组织病理学检查一致性极好(Kappa=0.890,P<0.05)。与单独液基细胞学检查比较,液基细胞hWAPL蛋白检测筛查宫颈癌的敏感度、准确率、阳性预测值、阴性预测值升高,漏诊率降低(均P<0.05)。结论:基于液基细胞学的hWAPL蛋白检验对HR-HPV感染患者宫颈癌的筛查效能较高。
Abstract:
Objective:To explore the value of human wings apart-like(hWAPL)protein testing based on liquid-based cytology in cervical cancer screening for patients with high-risk human papillomavirus(HR-HPV)infection.Methods:A total of 300 HPV-infected women were selected as the study subjects.Conventional HPV-DNA detection was performed using hybrid capture technology,cervical tissue specimens were taken under colposcopy for histopathological examination,and liquid-based cytology examination was conducted.Immunocytochemical technology was used to detect the expression of hWAPL protein in liquid-based cytology.Kappa test was used to analyze the consistency between individual liquid-based cytology examination,liquid-based cytology hWAPL protein testing and histopathological examination results,and to compare the screening efficacy of liquid-based cytology examination alone and liquid-based cytology hWAPL protein testing for cervical cancer in HR-HPV infected patients.Results:Among the 300 cervical liquid-based cytology specimens,a total of 18 HR-HPV subtypes and 5 low-risk HPV subtypes were detected,with 236 HR-HPV infected patients and an infection rate of 78.67%.Histopathological examination results showed that 64 cases were positive,with a positive rate of 27.12%.The results of individual liquid-based cytology examination showed that 50 patients were positive,with a positive rate of 21.19%,and the consistency with histopathological examination was high(Kappa=0.655,P<0.05).The results of liquid-based cytology hWAPL protein testing showed that 58 patients were positive,with a positive rate of 24.58%,and the consistency with histopathological examination was very good(Kappa=0.890,P<0.05).Compared with individual liquid-based cytology examination,the sensitivity,accuracy,positive predictive value,negative predictive value of liquid-based cytology hWAPL protein testing for cervical cancer screening were increased,and the missed diagnosis rate was reduced(all P<0.05).Conclusion:The hWAPL protein test based on liquid-based cytology has a high screening efficacy for cervical cancer in HR-HPV infected patients.

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备注/Memo

备注/Memo:
[基金项目]河北省医学科学研究课题(20200467,20220174); 河北省邯郸市科学技术研究与发展计划项目(1723208069-6)
更新日期/Last Update: 2025-02-04