[1]谭 蕊,李 论,罗 登.飞燕草素调控RhoA/ROCK信号通路对结肠癌细胞生物学行为及血管生成拟态形成的影响[J].陕西医学杂志,2024,(11):1468-1473.[doi:DOI:10.3969/j.issn.1000-7377.2024.11.006]
　TAN Rui,LI Lun,LUO Deng.Effects of delphinidin on the biological behavior and vasculogenic mimicry formation of colon cancer cells by regulating the RhoA/ROCK signaling pathway[J].,2024,(11):1468-1473.[doi:DOI:10.3969/j.issn.1000-7377.2024.11.006]
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飞燕草素调控RhoA/ROCK信号通路对结肠癌细胞生物学行为及血管生成拟态形成的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年11期
页码:
1468-1473
栏目:
基础研究
出版日期:
2024-11-05

文章信息/Info

Title:
Effects of delphinidin on the biological behavior and vasculogenic mimicry formation of colon cancer cells by regulating the RhoA/ROCK signaling pathway
作者:
谭 蕊; 李 论; 罗 登
(湖南中医药高等专科学校附属第一医院肛肠科,湖南 株洲 412000)
Author(s):
TAN Rui; LI Lun; LUO Deng
(Department of Anorectal,First Hospital Affiliated to Hunan Traditional Chinese Medical College,Zhuzhou 412000,China)
关键词:
结肠癌;  飞燕草素;  Ras同源基因家族成员A/Rho相关卷曲螺旋形成蛋白激酶信号通路;  恶性生物学行为;  血管生成拟态形成
Keywords:
Colon cancer;  Delphinidin;  RhoA/ROCK signaling pathway;  Malignant biological behavior;  Vasculogenic mimicry formation
分类号:
R 36
DOI:
DOI:10.3969/j.issn.1000-7377.2024.11.006
文献标志码:
A
摘要:
目的:探讨飞燕草素调控Ras同源基因家族成员A(RhoA)/Rho相关卷曲螺旋形成蛋白激酶(ROCK)信号通路对结肠癌细胞生物学行为及血管生成拟态形成的影响。方法:将人结肠癌细胞SW620随机分为对照组、飞燕草素低浓度组(含45 μmol/L飞燕草素培养基培养)、飞燕草素中浓度组(含90 μmol/L飞燕草素培养基培养)、飞燕草素高浓度组(含180 μmol/L飞燕草素培养基培养)、飞燕草素高浓度+RhoA过表达组(转染RhoA过表达质粒+含180 μmol/L飞燕草素培养基培养)。采用细胞计数试剂盒-8(CCK-8)法、Transwell及划痕实验分别检测SW620细胞增殖、侵袭及迁移能力。采用流式细胞术检测SW620细胞凋亡情况。血管生成实验检测SW620细胞血管生成拟态形成情况。采用RT-qPCR检测SW620细胞RhoA、ROCK mRNA表达。采用Western blot检测SW620细胞RhoA、ROCK、血管内皮生长因子(VEGF)蛋白表达。结果:与对照组比较,飞燕草素低、中、高浓度组SW620细胞存活率、侵袭细胞数、迁移率、血管生成拟态管状结构数、RhoA和ROCK mRNA及蛋白表达水平、VEGF蛋白表达水平呈浓度依赖性降低,凋亡率呈浓度依赖性升高(均P<0.05)。与飞燕草素高浓度组比较,飞燕草素高浓度+RhoA过表达组SW620细胞存活率、侵袭细胞数、迁移率、血管生成拟态管状结构数、RhoA和ROCK mRNA及蛋白表达水平、VEGF蛋白表达水平升高,凋亡率降低(均P<0.05)。结论:飞燕草素可能通过抑制RhoA/ROCK信号通路减弱结肠癌细胞恶性生物学行为及血管生成拟态形成,并促进细胞凋亡。
Abstract:
Objective:To investigate the effects of delphinidin on the biological behavior and vasculogenic mimicry formation of colon cancer cells by regulating the RhoA/ROCK signaling pathway.Methods:Human colon cancer cells SW620 were randomly divided into the control group,delphinidin low-concentration group(culture medium containing 45 μmol/L delphinium),delphinidin medium-concentration group(culture medium containing 90 μmol/L delphinium),delphinidin high-concentration group(culture medium containing 180 μmol/L delphinium),and delphinidin high-concentration+RhoA overexpression group(transfected RhoA overexpression plasmid+culture medium containing 180 μmol/L delphinium).The proliferation,invasion and migration abilities of SW620 cells were assessed using the cell counting kit-8(CCK-8),Transwell and scratch assays,respectively.The apoptosis of SW620 cells was detected by flow cytometry.The formation of angiogenic mimicry in SW620 cells was detected by angiogenesis experiment.The expression of RhoA and ROCK mRNA in SW620 cells was detected by RT-qPCR.Western blot was used to detect RhoA,ROCK and vascular endothelial growth factor(VEGF)protein expression in SW620 cells.Results:Compared with the control group,the survival rate,number of invasive cells,migration rate,number of vasculogenic mimicry tubular structures,mRNA and protein expression levels of RhoA and ROCK and VEGF protein expression level in the delphinidin low,medium and high-concentration groups decreased in a concentration-dependent manner,while the apoptosis rate increased in a concentration-dependent manner(all P<0.05).Compared with the delphinidin high-concentration group,the survival rate,number of invasive cells,migration rate,number of vasculogenic mimicry tubular structures,mRNA and protein expression levels of RhoA and ROCK and VEGF protein expression level in the delphinidin high-concentration+RhoA overexpression group increased,and the apoptosis rate decreased(all P<0.05).Conclusion:Delphinidin may suppress the malignant biological behavior and vasculogenic mimicry formation of colon cancer cells and promote apoptosis by inhibiting the RhoA/ROCK signaling pathway.

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备注/Memo

备注/Memo:
基金项目:湖南省卫生健康委员会科研立项课题(20230360)
更新日期/Last Update: 2024-11-04