[1]王银燕,黄 翠,朱 艳,等.长链非编码RNA UBE2Q1-AS1对食管癌细胞增殖和迁移的调节作用及机制实验研究[J].陕西医学杂志,2024,(9):1172-1176,1181.[doi:DOI:10.3969/j.issn.1000-7377.2024.09.004]
　WANG Yinyan,HUANG Cui,ZHU Yan,et al.Regulatory effect and mechanism of lncRNA UBE2Q1-AS1 on proliferation and migration of esophageal cancer cells[J].,2024,(9):1172-1176,1181.[doi:DOI:10.3969/j.issn.1000-7377.2024.09.004]
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长链非编码RNA UBE2Q1-AS1对食管癌细胞增殖和迁移的调节作用及机制实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年9期
页码:
1172-1176,1181
栏目:
基础研究
出版日期:
2024-09-05

文章信息/Info

Title:
Regulatory effect and mechanism of lncRNA UBE2Q1-AS1 on proliferation and migration of esophageal cancer cells
作者:
王银燕1; 黄 翠1; 朱 艳1; 朱 磊2
(1.黄石市妇幼保健院综合内科,湖北 黄石 435000; 2.四川大学华西医院肿瘤科,四川 成都 610044)
Author(s):
WANG Yinyan; HUANG Cui; ZHU Yan; ZHU Lei
(Department of Comprehensive Internal Medicine,Huangshi Maternal and Child Health Hospital,Huangshi 435000,China)
关键词:
食管癌;  长链非编码RNA;  UBE2Q1-AS1;  微小RNA-377-3p;  细胞增殖;  细胞迁移
Keywords:
Esophageal cancer;  Long non-coding RNA;  UBE2Q1-AS1;  miR-377-3p;  Cell proliferation;  Cell migration
分类号:
R 73-3
DOI:
DOI:10.3969/j.issn.1000-7377.2024.09.004
文献标志码:
A
摘要:
目的:观察长链非编码RNA(lncRNA)UBE2Q1-AS1对食管癌细胞增殖和迁移的影响,并探讨UBE2Q1-AS1发挥调节作用的分子机制。方法:实时荧光定量PCR(RT-qPCR)检测食管癌EC9706、Eca109、KYSE-510、TE-13细胞和永生化食管上皮HET-1A细胞中UBE2Q1-AS1的表达。将KYSE-510细胞分为si-NC组和si-UBE2Q1-AS1组,分别转染si-NC质粒和si-UBE2Q1-AS1质粒。集落形成实验检测各组KYSE-510细胞增殖活性。细胞划痕实验检测各组KYSE-510细胞的迁移能力。双荧光素酶报告系统验证UBE2Q1-AS1与微小RNA(miR)-377-3p的靶向关系。RT-qPCR检测各组KYSE-510细胞中miR-377-3p相对表达量。蛋白质印迹法(Western blot)检测各组KYSE-510细胞中同源盒蛋白A1(HOXA1)、纤连蛋白(Fibronectin)、叉头相关转录因子C2(FOXC2)、粒状蛋白质2同源物(GRHL2)和E-钙黏蛋白(E-cadherin)蛋白的相对表达量。结果:与HET-1A细胞比较,UBE2Q1-AS1在食管癌细胞中表达均升高(P<0.01)。si-UBE2Q1-AS1组KYSE-510细胞增殖活性低于si-NC组(P<0.01)。si-UBE2Q1-AS1组KYSE-510细胞迁移能力低于si-NC组(P<0.01)。UBE2Q1-AS1与miR-377-3p间存在靶向关系(P<0.01)。si-UBE2Q1-AS1组KYSE-510细胞中miR-377-3p表达水平高于si-NC组(P<0.01)。与si-NC组比较,si-UBE2Q1-AS1组KYSE-510细胞中HOXA1、Fibronectin、FOXC2蛋白表达降低,GRHL2和E-cadherin蛋白表达升高(均P<0.01)。结论:在食管癌细胞中,UBE2Q1-AS1表达上调,敲低UBE2Q1-AS1可能通过调节miR-377-3p/HOXA1轴抑制食管癌细胞增殖和迁移。
Abstract:
Objective:To observe the effect of long non-coding RNA(lncRNA)UBE2Q1-AS1 on the proliferation and migration of esophageal cancer cells,and to explore the molecular mechanism by which UBE2Q1-AS1 exerts its regulatory effect.Methods:RT-qPCR was used to detect the expression of UBE2Q1-AS1 in esophageal cancer EC9706,Eca109,KYSE-510,TE-13 cells and immortalized esophageal epithelial HET-1A cells.KYSE-510 cells were divided into si-NC group and si-UBE2Q1-AS1 group,and were transfected with si-NC plasmid and si-UBE2Q1-AS1 plasmid respectively.Colony formation experiment was used to detect the proliferation activity of KYSE-510 cells in each group.The scratch experiment was used to detect the migration ability of KYSE-510 cells in each group.The dual-luciferase reporter system was used to verify the targeting relationship between UBE2Q1-AS1 and miR-377-3p.RT-qPCR was used to detecte the expression of miR-377-3p in KYSE-510 cells in each group.Western blot was used to detecte the expression of homeobox protein A1(HOXA1),fibronectin,forkhead box protein C2(FOXC2),grainyhead-like protein 2 homolog(GRHL2)and E-cadherin proteins in KYSE-510 cells in each group.Results:Compared with HET-1A cells,UBE2Q1-AS1 expression was increased in esophageal cancer cells(P<0.01).The proliferation activity of KYSE-510 cells in the si-UBE2Q1-AS1 group was lower than that in the si-NC group(P<0.01).The migration ability of KYSE-510 cells in the si-UBE2Q1-AS1 group was lower than that in the si-NC group(P<0.01).There was a targeting relationship between UBE2Q1-AS1 and miR-377-3p(P<0.01).The expression level of miR-377-3p in KYSE-510 cells in the si-UBE2Q1-AS1 group was higher than that in the si-NC group(P<0.01).Compared with the si-NC group,the protein expressions of HOXA1,Fibronectin,and FOXC2 in KYSE-510 cells in the si-UBE2Q1-AS1 group decreased,and the protein expressions of GRHL2 and E-cadherin increased(all P<0.01).Conclusion:UBE2Q1-AS1 expression is up-regulated in esophageal cancer cells,and UBE2Q1-AS1 down-regulation may inhibit the proliferation and migration of esophageal cancer cells by regulating the miR-377-3p/HOXA1 axis.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81902819)
更新日期/Last Update: 2024-09-04