[1]孟凡岗,刘文宇,甄立军,等.原发性肝癌组织长链非编码RNA JPX、微小RNA-371a-5p表达水平及其与患者术后5年预后关系研究[J].陕西医学杂志,2024,(8):1130-1134,1138.[doi:DOI:10.3969/j.issn.1000-7377.2024.08.027]
　MENG Fangang,LIU Wenyu,ZHEN Lijun,et al.Expression levels of long non-coding RNA JPX and microRNA-371a-5p in primary liver cancer tissues and their relationship with 5-year prognosis of patients after surgery[J].,2024,(8):1130-1134,1138.[doi:DOI:10.3969/j.issn.1000-7377.2024.08.027]
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原发性肝癌组织长链非编码RNA JPX、微小RNA-371a-5p表达水平及其与患者术后5年预后关系研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年8期
页码:
1130-1134,1138
栏目:
临床病理
出版日期:
2024-08-05

文章信息/Info

Title:
Expression levels of long non-coding RNA JPX and microRNA-371a-5p in primary liver cancer tissues and their relationship with 5-year prognosis of patients after surgery
作者:
孟凡岗; 刘文宇; 甄立军; 修 娜; 陈 飞
(大庆龙南医院普外一科,黑龙江 大庆 163000)
Author(s):
MENG Fangang; LIU Wenyu; ZHEN Lijun; XIU Na; CHEN Fei
(First Department of General Surgery,Da Qing Long Nan Hospital,Daqing 163000,China)
关键词:
原发性肝癌;  长链非编码RNA JPX;  微小RNA-371a-5p;  相关性;  预后;  预测价值
Keywords:
Primary liver cancer;  Long non-coding RNA JPX;  miR-371a-5p;  Correlation;  Prognosis;  Predictive value
分类号:
R 735.7
DOI:
DOI:10.3969/j.issn.1000-7377.2024.08.027
文献标志码:
A
摘要:
目的:探讨原发性肝癌组织长链非编码RNA(lncRNA)JPX、微小RNA-371a-5p(miR-371a-5p)表达水平及其与患者术后5年预后的关系。方法:选取行根治术治疗的105例原发性肝癌患者的癌组织以及距离癌组织3 cm的癌旁正常组织。RT-qPCR检测组织中lncRNA JPX、miR-371a-5p表达水平。双荧光素酶报告基因实验验证lncRNA JPX与miR-371a-5p的靶向关系。术后随访5年根据生存情况将患者分为生存组(64例)和病死组(41例)。比较肝癌组织和癌旁正常组织lncRNA JPX、miR-371a-5p表达水平。比较生存组和病死组临床病理特征及lncRNA JPX、miR-371a-5p水平。Pearson法分析lncRNA JPX、miR-371a-5p水平与肝内转移、肿瘤分化程度、中国肝癌分期(CNLC)、血管侵犯的相关性。多因素Cox回归分析原发性肝癌患者术后5年预后影响因素。绘制受试者工作特征(ROC)曲线分析肝癌组织lncRNA JPX、miR-371a-5p对患者术后5年生存的预测价值。结果:肝癌组织中lncRNA JPX、miR-371a-5p表达水平较癌旁正常组织升高(均P<0.05)。lncRNA JPX可正向调控miR-371a-5p表达。lncRNA JPX、miR-371a-5p高表达患者5年总生存率分别低于lncRNA JPX、miR-371a-5p低表达患者(均P<0.05)。生存组无肝内转移、肿瘤高/中分化、CNLC Ⅰ-Ⅱ期、无血管侵犯患者比例高于病死组,且lncRNA JPX和miR-371a-5p表达水平低于病死组(均P<0.05)。有肝内转移、肿瘤低分化、CNLC Ⅲa期、有血管侵犯、lncRNA JPX高表达、miR-371a-5p高表达是原发性肝癌患者术后5年生存的独立危险因素(均P<0.05)。肝癌组织lncRNA JPX与miR-371a-5p呈正相关,两者与肝内转移、CNLC分期、血管侵犯呈正相关,与肿瘤分化程度呈负相关(均P<0.05)。 lncRNA JPX、miR-371a-5p联合预测患者术后5年生存的曲线下面积(AUC)大于lncRNA JPX及miR-371a-5p单独预测的AUC(均P<0.05)。结论:原发性肝癌组织lncRNA JPX、miR-371a-5p呈高表达,lncRNA JPX可正向调控miR-371a-5p,两者是患者术后5年生存的独立危险因素,对术后5年生存的预测价值较高。
Abstract:
Objective:To investigate the expression levels of long non-coding RNA(lncRNA)JPX and microRNA-371a-5p(miR-371a-5p)in primary liver cancer tissues and their relationship with 5-year prognosis of patients after surgery.Methods:Cancer tissues and adjacent normal tissues 3 cm away from the cancer tissues of 105 patients with primary liver cancer who underwent radical surgery were collected.The expression levels of lncRNA JPX and miR-371a-5p in the tissues were detected by RT-qPCR.Dual luciferase reporter gene assay verified the targeting relationship between lncRNA JPX and miR-371a-5p.After 5 years of postoperative follow-up,the patients were divided into survival group(64 cases)and death group(41 cases).The expression levels of lncRNA JPX and miR-371a-5p in liver cancer and adjacent normal tissues were compared.The clinicopathological characteristics and lncRNA JPX and miR-371a-5p levels of survival group and death group were compared.The correlation of lncRNA JPX and miR-371a-5p levels with intrahepatic metastasis,tumor differentiation,China liver cancer staging(CNLC),and vascular invasion was analyzed by Pearson method.Multivariate Cox regression analysis was performed to analyze the prognostic factors of patients with primary liver cancer at 5 years after operation.Receiver operating characteristic(ROC)curve was drawn to analyze the predictive value of lncRNA JPX and miR-371a-5p on 5-year postoperative survival of patients with liver cancer.Results:The expression levels of lncRNA JPX and miR-371a-5p in liver cancer tissues were higher than those in adjacent normal tissues(all P<0.05).lncRNA JPX could positively regulate the expression of miR-371a-5p.The 5-year overall survival rate of lncRNA JPX and miR-371a-5p patients with high expression was lower than that of lncRNA JPX and miR-371a-5p patients with low expression(all P<0.05).The proportions of patients without intrahepatic metastasis,high/medium tumor differentiation,CNLC stageⅠ-Ⅱ,and no vascular invasion in survival group were higher than those in death group,and the expression levels of lncRNA JPX and miR-371a-5p were lower than those in death group(all P<0.05).Intrahepatic metastasis,low tumor differentiation,CNLC stage Ⅲa,vascular invasion,high lncRNA JPX expression and high miR-371a-5p expression were independent risk factors for 5-year survival of patients with primary liver cancer after surgery(all P<0.05).There was a positive correlation between lncRNA JPX and miR-371a-5p in liver cancer tissues,and both were positively correlated with intrahepatic metastasis,CNLC stage and vascular invasion,and negatively correlated with tumor differentiation(all P<0.05).The area under the curve(AUC)predicted by the combination of lncRNA JPX and miR-371a-5p for 5-year postoperative survival was greater than that predicted by lncRNA JPX and miR-371a-5p alone(all P<0.05).Conclusion:LncRNA JPX and miR-371a-5p are highly expressed in primary liver cancer tissues,and lncRNA JPX can positively regulate miR-371a-5p.Both are independent risk factors for 5-year postoperative survival of patients,and have high value in predicting 5-year postoperative survival.

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备注/Memo

备注/Memo:
基金项目:黑龙江省卫生健康委员会科技计划项目(20220909010135)
更新日期/Last Update: 2024-08-08