[1]王瑜崴,李 濛,康 博,等.结肠癌组织微小RNA-3607-3p、细胞周期蛋白E2表达及其与患者术后5年预后关系研究[J].陕西医学杂志,2024,(8):1124-1129.[doi:DOI:10.3969/j.issn.1000-7377.2024.08.026]
　WANG Yuwei,LI Meng,KANG Bo,et al.Expression of microRNA-3607-3p and cyclin E2 in colon cancer tissues and their relationship with 5-year prognosis of patients after surgery[J].,2024,(8):1124-1129.[doi:DOI:10.3969/j.issn.1000-7377.2024.08.026]
点击复制

结肠癌组织微小RNA-3607-3p、细胞周期蛋白E2表达及其与患者术后5年预后关系研究
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年8期
页码:
1124-1129
栏目:
临床病理
出版日期:
2024-08-05

文章信息/Info

Title:
Expression of microRNA-3607-3p and cyclin E2 in colon cancer tissues and their relationship with 5-year prognosis of patients after surgery
作者:
王瑜崴1; 李 濛2; 康 博3; 杨 超4
(1.牡丹江市肿瘤医院腹外一科,黑龙江 牡丹江 157009; 2.牡丹江市肿瘤医院放疗二科,黑龙江 牡丹江 157009; 3.牡丹江市肿瘤医院肿瘤内一科,黑龙江 牡丹江 157009; 4.牡丹江医学院附属第二医院康复医学科,黑龙江 牡丹江 157011)
Author(s):
WANG Yuwei; LI Meng; KANG Bo; YANG Chao
(First Department of Abdominal Surgery,Mudanjiang Tumor Hospital,Mudanjiang 157009,China)
关键词:
结肠癌;  微小RNA-3607-3p;  细胞周期蛋白E2;  临床病理特征;  相关性;  预后;  预测价值
Keywords:
Colon cancer;  miR-3607-3p;  Cyclin E2;  Clinicopathological characteristics;  Correlation;  Prognosis;  Predictive value
分类号:
R 735.35
DOI:
DOI:10.3969/j.issn.1000-7377.2024.08.026
文献标志码:
A
摘要:
目的:探讨结肠癌组织微小RNA-3607-3p(miR-3607-3p)、细胞周期蛋白E2(CCNE2)表达及其与患者术后5年预后的关系。方法:选取行结肠癌根治术治疗的88例结肠癌患者癌组织和癌旁组织。RT-qPCR法测定组织miR-3607-3p、CCNE2 mRNA表达。免疫组化法检测组织CCNE2蛋白表达。随访术后5年内结肠癌患者生存情况,根据生存情况将患者分为生存组(48例)和病死组(40例)。分析miR-3607-3p、CCNE2 mRNA与TNM分期、肿瘤分化程度、淋巴结转移的相关性。Cox回归分析结肠癌患者术后5年预后的影响因素。绘制受试者工作特征(ROC)曲线分析miR-3607-3p、CCNE2 mRNA对患者术后5年内生存的预测价值。结果:结肠癌组织miR-3607-3p表达水平低于癌旁组织,CCNE2 mRNA表达水平和CCNE2蛋白阳性表达率高于癌旁组织(均P<0.05)。miR-3607-3p低表达和CCNE2 mRNA高表达患者TNM分期Ⅲ期、肿瘤低分化、有淋巴结转移的占比更高(均P<0.05)。结肠癌组织miR-3607-3p与CCNE2 mRNA、TNM分期、淋巴结转移呈负相关,与肿瘤分化程度呈正相关(均P<0.05)。CCNE2 mRNA与TNM分期、淋巴结转移呈正相关,与肿瘤分化程度呈负相关(均P<0.05)。miR-3607-3p高表达、CCNE2 mRNA低表达患者5年总生存率高于miR-3607-3p低表达、CCNE2 mRNA高表达患者(均P<0.05)。病死组TNM分期Ⅲ期、肿瘤低分化、有淋巴结转移的患者占比更高(均P<0.05)。病死组结肠癌组织CCNE2 mRNA和蛋白阳性表达率高于生存组,miR-3607-3p低于生存组(均P<0.05)。TNM分期Ⅲ期、肿瘤低分化、有淋巴结转移、miR-3607-3p低表达、CCNE2 mRNA高表达、CCNE2蛋白阳性表达是影响结肠癌患者术后5年生存的独立危险因素(均P<0.05)。结肠癌组织miR-3607-3p、CCNE2 mRNA两者联合预测患者术后5年内生存的曲线下面积(AUC)高于miR-3607-3p、CCNE2 mRNA单独预测的AUC(均P<0.05)。结论:结肠癌组织miR-3607-3p呈低表达,CCNE2呈高表达,两者与患者术后5年预后有关,有望成为结肠癌患者术后5年内生存的预测指标。
Abstract:
Objective:To investigate the expression of microRNA-3607-3p(miR-3607-3p)and cyclin E2(CCNE2)in colon cancer tissues and their relationship with the 5-year prognosis of patients after surgery.Methods:The cancer tissues and paracancerous tissues of 88 colon cancer patients who underwent radical colon cancer resection were selected. The mRNA expressions of miR-3607-3p and CCNE2 in tissues were determined by RT-qPCR. Immunohistochemistry was used to detect the expression of CCNE2 protein in tissues. The survival of colon cancer patients within 5 years after surgery was followed-up, and the patients were divided into survival group(48 cases)and death group(40 cases)according to the survival situation. The correlation between miR-3607-3p and CCNE2 mRNA and TNM stage, tumor differentiation and lymph node metastasis was analyzed. Cox regression analysis was performed to analyze the influencing factors of 5-year prognosis of patients with colon cancer after surgery. The receiver operating characteristic(ROC)curve was drawn to analyze the predictive value of miR-3607-3p and CCNE2 mRNA for the survival of patients within 5 years after surgery.Results:The expression level of miR-3607-3p in colon cancer tissues was lower than that in paracancerous tissues,and the expression level of CCNE2 mRNA and the positive expression rate of CCNE2 protein were higher than those in paracancerous tissues(all P<0.05).Patients with low miR-3607-3p expression and high CCNE2 mRNA expression had higher proportions of TNM stage Ⅲ,poor tumor differentiation and lymph node metastasis(all P<0.05).MiR-3607-3p in colon cancer tissues was negatively correlated with CCNE2 MRNA,TNM stage and lymph node metastasis,and positively correlated with tumor differentiation degree(all P<0.05).CCNE2 mRNA was positively correlated with TNM stage and lymph node metastasis,and negatively correlated with tumor differentiation degree(all P<0.05).The 5-year overall survival rate of patients with high miR-3607-3p expression and low CCNE2 mRNA expression was higher than that of patients with low miR-3607-3p expression and high CCNE2 mRNA expression(all P<0.05).The proportion of patients with TNM stage Ⅲ,poor tumor differentiation and lymph node metastasis in the death group was higher(all P<0.05).The expression level of CCNE2 mRNA and the positive expression rate of CCNE2 protein in colon cancer tissues in death group were higher than those in the survival group,and miR-3607-3p was lower than that in survival group(all P<0.05).TNM stage Ⅲ,poor tumor differentiation,lymph node metastasis,low expression of miR-3607-3p,high expression of CCNE2 mRNA,and positive expression of CCNE2 protein were all independent risk factors affecting the 5-year survival of patients with colon cancer after surgery(all P<0.05).The AUC of the combination of miR-3607-3p and CCNE2 mRNA in colon cancer tissue to predict the survival of patients within 5 years after surgery was higher than that of miR-3607-3p or CCNE2 mRNA alone(all P<0.05).Conclusion:The low expression of miR-3607-3p and high expression of CCNE2 in colon cancer tissues are related to the 5-year prognosis of patients with colon cancer,which are expected to be predictive indicators for the 5-year survival of patients with colon cancer.

参考文献/References:

[1] 王江业,张倍宁,孙国良,等.温阳扶正方治疗晚期结肠癌脾肾阳虚证临床研究[J].陕西中医,2024,45(2):196-199.
[2] TAO L,SONG P,SHAO L,et al.MiR-129-2-3p inhibits colon cancer cell proliferation by down-regulating the expression of BZW1[J].Arab J Gastroenterol,2024,25(1):42-50.
[3] YUN Z Y,WU D,WANG X,et al.MiR-214-3p overexpression-triggered chondroitin polymerizing factor(CHPF)inhibition modulates the ferroptosis and metabolism in colon cancer[J].Kaohsiung J Med Sci,2024,40(3):244-254.
[4] 张文娟,王琰琰,周静祎,等.微小RNA-181c-5p和Kruppel样因子6在子宫内膜样腺癌组织中的表达及其与患者临床病理特征关系研究[J].陕西医学杂志,2024,53(4):552-555.
[5] 荆春霞,袁高亮,于蕾,等.miR-497-5p、miR-3607-3p与宫颈癌合并高危型HPV感染的相关性分析[J].中国艾滋病性病,2024,30(2):167-171.
[6] DENG Y,HUANG H,SHI J,et al.Identification of candidate genes in breast cancer induced by estrogen plus progestogens using bioinformatic analysis[J].Int J Mol Sci,2022,23(19):11892-11915.
[7] ZHANG X,YANG L,HUANG B,et al.Identification and validation of cyclin A2 and cyclin E2 as potential biomarkers in small cell lung cancer[J].Oncol Res Treat,2023,46(6):246-258.
[8] WANG Q,WU H,WU Q,et al.Berberine targets KIF20A and CCNE2 to inhibit the progression of nonsmall cell lung cancer via the PI3K/AKT pathway[J].Drug Dev Res,2023,84(5):907-921.
[9] GAO P,WANG H,YU J,et al.MiR-3607-3p suppresses non-small cell lung cancer(NSCLC)by targeting TGFBR1 and CCNE2[J].PLoS Genet,2018,14(12):e1007790.
[10] 中华人民共和国卫生部.结肠癌规范化诊疗指南(试行)[J].慢性病学杂志,2013,14(7):481-485.
[11] 万里,程如焕,邹绍静,等.结肠癌组织中HIF-1α和FoxM1的表达及其与临床病理特征和预后关系的研究[J].国际消化病杂志,2022,42(5):294-299.
[12] MONTMINY E M,ZHOU M,LONG C,et al.Impact of carcinoid and appendiceal site inclusion on early onset colon cancer incidence rate analysis in the United States[J].Clin Gastroenterol Hepatol,2023,21(11):2972-2974.
[13] 徐敏,王彦舟,张华.全身炎性反应指数联合控制营养状况评分对结肠癌患者预后的预测价值[J].陕西医学杂志,2023,52(11):1558-1562,1566.
[14] MO T W,ZHANG Z J,CHEN Y L,et al.Risk factors for metachronous peritoneal carcinomatosis after radical resection for patients with nonmetastatic pT3-4 colon cancer[J].J Surg Oncol,2022,126(4):757-771.
[15] YAN J,ZHAO Z,WANG L,et al.Construction of predictive model for prognosis of patients after radical resection of colon cancer based on nomogram[J].Am J Transl Res,2023,15(4):2783-2792.
[16] JI Y,GU J,ZHANG H,et al.Identification and validation of key miRNAs for colon cancer based on miRNA-gene integration analysis[J].Int J Gen Med,2023,16:5703-5718.
[17] 张琳,邓贵华,杨亮,等.苦木碱F通过miR-331-3p调控宫颈癌 HeLa细胞增殖及凋亡的机制研究[J].陕西中医,2022,43(1):17-22.
[18] LOU W,CHEN J,DING B,et al.XIAP,commonly targeted by tumor suppressive miR-3607-5p and miR-3607-3p,promotes proliferation and inhibits apoptosis in hepatocellular carcinoma[J].Genomics,2021,113(3):933-945.
[19] 陈一蕊,勾朝阳,李新阳.宫颈癌患者血清miR-3607-3p水平与人乳头瘤病毒感染相关性[J].中国计划生育学杂志,2022,30(2):470-473.
[20] TAO K,LIU J,LIANG J,et al.Vascular endothelial cell-derived exosomal miR-30a-5p inhibits lung adenocarcinoma malignant progression by targeting CCNE2[J].Carcinogenesis,2021,42(8):1056-1067.
[21] WANG J F,XI Z N,SU H J,et al.SP1-induced overexpression of LINC00520 facilitates non-small cell lung cancer progression through miR-577/CCNE2 pathway and predicts poor prognosis[J].Hum Cell,2021,34(3):952-964.
[22] WELSCH E,SCHUSTER E,KRAINER M,et al.Comparison of RNA marker panels for circulating tumor cells and evaluation of their prognostic relevance in breast cancer[J].Cancers(Basel),2023,15(4):1271-1288.
[23] SHEN J,HU J,WU J,et al.Molecular characterization of long-term survivors of hepatocellular carcinoma[J].Aging(Albany NY),2021,13(5):7517-7537.
[24] 王江波,梁皓,陆允平.miRNA-144-3p靶向CCNE2调控肺腺癌细胞周期并抑制其增殖[J].实用药物与临床,2023,26(6):481-487.
[25] LI H,FENG H,ZHANG T,et al.CircHAS2 activates CCNE2 to promote cell proliferation and sensitizes the response of colorectal cancer to anlotinib[J].Mol Cancer,2024,23(1):59-77.

相似文献/References:

[1]王 雄,张月飞,赵东雄,等.初诊结肠癌患者血小板相关指标与临床分期关系分析[J].陕西医学杂志,2019,(12):1676.
[2]龚 磊,吕真冰,喻 晶,等.SIRT1、Phospho-SIRT1在结肠癌组织中的表达及临床意义*[J].陕西医学杂志,2020,49(3):274.
　GONG Lei,LV Zhenbing,YU Jing,et al.Expression and clinical significance of SIRT1 and Phospho-SIRT1 in colon cancer tissues[J].,2020,49(8):274.
[3]冯 婷,刘 霞,高立勇.叉头框转录因子O亚族3a对结肠癌SW620细胞的影响及机制研究[J].陕西医学杂志,2021,50(9):1068.[doi:DOI:10.3969/j.issn.1000-7377.2021.09.007]
　FENG Ting,LIU Xia,GAO Liyong.Effect and mechanism of FOXO3a on colon cancer SW620 cells[J].,2021,50(8):1068.[doi:DOI:10.3969/j.issn.1000-7377.2021.09.007]
[4]顾 勇,杨 艳,符 翠,等.白藜芦醇对结肠癌恶性生物学行为的抑制作用研究[J].陕西医学杂志,2021,50(9):1072.[doi:DOI:10.3969/j.issn.1000-7377.2021.09.008]
　GU Yong,YANG Yan,FU Cui,et al.Inhibitory effect of resveratrol on malignant biological behavior of colon cancer[J].,2021,50(8):1072.[doi:DOI:10.3969/j.issn.1000-7377.2021.09.008]
[5]余 亮,郭 建,周国强,等.结肠癌患者血清微小RNA-193a-5p和微小RNA-139-5p表达检测及其对预后的影响[J].陕西医学杂志,2023,52(6):745.[doi:DOI:10.3969/j.issn.1000-7377.2023.06.024]
　YU Liang,GUO Jian,ZHOU Guoqiang,et al.Expression of serum miR-193a-5p and miRNA-139-5p in patients with colon cancer and their effects on prognosis[J].,2023,52(8):745.[doi:DOI:10.3969/j.issn.1000-7377.2023.06.024]
[6]徐 敏,王彦舟,张 华.全身炎性反应指数联合控制营养状况评分对结肠癌患者预后的预测价值[J].陕西医学杂志,2023,52(11):1558.[doi:DOI:10.3969/j.issn.1000-7377.2023.11.023]
　XU Min,WANG Yanzhou,ZHANG Hua.Predictive value of SIRI combined with CONUT score in prognosis of patients with colon cancer[J].,2023,52(8):1558.[doi:DOI:10.3969/j.issn.1000-7377.2023.11.023]
[7]谭 蕊,李 论,罗 登.飞燕草素调控RhoA/ROCK信号通路对结肠癌细胞生物学行为及血管生成拟态形成的影响[J].陕西医学杂志,2024,(11):1468.[doi:DOI:10.3969/j.issn.1000-7377.2024.11.006]
　TAN Rui,LI Lun,LUO Deng.Effects of delphinidin on the biological behavior and vasculogenic mimicry formation of colon cancer cells by regulating the RhoA/ROCK signaling pathway[J].,2024,(8):1468.[doi:DOI:10.3969/j.issn.1000-7377.2024.11.006]

备注/Memo

备注/Memo:
基金项目:黑龙江省卫生健康委员会科研课题(20210505020120)
更新日期/Last Update: 2024-08-08