[1]司 晗,李洁妹,宗佳琪,等.高糖环境对卵巢癌细胞侵袭和迁移的影响及其机制实验研究[J].陕西医学杂志,2024,(8):1016-1020.[doi:DOI:10.3969/j.issn.1000-7377.2024.08.002]
　SI Han,LI Jiemei,ZONG Jiaqi,et al.Effect of high glucose environment on the invasion and migration of ovarian cancer cells and its mechanism[J].,2024,(8):1016-1020.[doi:DOI:10.3969/j.issn.1000-7377.2024.08.002]
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高糖环境对卵巢癌细胞侵袭和迁移的影响及其机制实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年8期
页码:
1016-1020
栏目:
基础研究
出版日期:
2024-08-05

文章信息/Info

Title:
Effect of high glucose environment on the invasion and migration of ovarian cancer cells and its mechanism
作者:
司 晗1; 2; 李洁妹1; 2; 宗佳琪1; 2; 杨夏楠2; 赵丹丹2; 樊冬梅1
(1.河南科技大学第一附属医院妇科,河南 洛阳 471000; 2.河南科技大学临床医学院,河南 洛阳471000)
Author(s):
SI Han; LI Jiemei; ZONG Jiaqi; YANG Xia'nan; ZHAO Dandan; FAN Dongmei
(Department of Gynaecology,the First Affiliated Hospital ofHenan University of Science and Technology,Luoyang 471000,China)
关键词:
卵巢癌;  高糖;  氧化应激;  细胞外信号调节激酶/核因子-κB信号通路;  侵袭;  迁移
Keywords:
Ovarian cancer;  High glucose;  Oxidative stress;  Extracellular signal-regulated kinase/nuclear factor-κB signaling pathway;  Invasion;  Migration
分类号:
R 36
DOI:
DOI:10.3969/j.issn.1000-7377.2024.08.002
文献标志码:
A
摘要:
目的:探究高糖环境对卵巢癌细胞侵袭和迁移的影响及其可能的作用机制。方法:将人卵巢癌细胞系SKOV-3分为正常糖组(低糖型培养基培养)、高糖组(高糖型培养基培养)、高糖+过氧化氢酶(CAT)组(高糖型培养基+400 U/ml CAT培养)、高糖+丝裂原活化的细胞外信号调节激酶(MEK)抑制剂PD98059组(高糖型培养基+50 μmol/ml PD98059培养)。采用过氧化氢(H2O2)检测试剂盒测定细胞内H2O2水平。划痕实验检测细胞迁移能力。Transwell实验检测细胞侵袭能力。免疫印迹法检测细胞E钙黏蛋白(E-cadherin)、N钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)、细胞外信号调节激酶(ERK)、p-ERK、核因子-κB(NF-κB)、p-NF-κB蛋白表达。RT-qPCR检测细胞E-cadherin、N-cadherin、Vimentin mRNA表达。结果:与正常糖组比较,高糖组H2O2水平、24 h细胞迁移率、侵袭细胞数、p-ERK、p-NF-κB蛋白表达水平和N-cadherin、Vimentin蛋白及mRNA表达水平升高,E-cadherin蛋白及mRNA表达水平降低(均P<0.01)。与高糖组比较,高糖+CAT组H2O2水平降低,高糖+CAT组和高糖+PD98059组24 h细胞迁移率、侵袭细胞数、p-ERK及p-NF-κB蛋白表达水平和N-cadherin、Vimentin蛋白及mRNA表达水平降低,E-cadherin蛋白及mRNA表达水平升高(均P<0.01)。结论:高糖能够诱导卵巢癌细胞产生H2O2并激活下游ERK/NF-κB信号通路,促进卵巢癌细胞的侵袭和迁移,并影响卵巢癌细胞上皮-间充质转化(EMT)相关因子表达。
Abstract:
Objective:To investigate the effect of high glucose environment on invasion and migration of ovarian cancer cells and its possible mechanism.Methods:Human ovarian cancer cell line SKOV-3 was divided into normal glucose group(low glucose medium culture),high glucose group(high glucose medium culture),high glucose+catalase(CAT)group(high glucose medium+400 U/ml CAT culture),and high glucose+mitogen-actived extracellular signal-regulated kinase(MEK)inhibitor PD98059 group(high glucose medium+50 μmol/ml PD98059 culture).Intracellular hydrogen peroxide(H2O2)levels were measured by H2O2 assay kit.Cell migration ability was detected by scratch test.Transwell assay was used to detect cell invasion ability.The expression of E-cadherin,N-cadherin,Vimentin,extracellular signal-regulated kinase(ERK),p-ERK,nuclear factor-κB(NF-κB)and p-NF-κB proteins was detected by Western blot.The expression of E-cadherin,N-cadherin and Vimentin mRNA was detected by RT-qPCR.Results:Compared with normal glucose group,H2O2 level,24 h cell migration rate,number of invading cells,the expression levels of p-ERK,p-NF-κB proteins and the protein and mRNA expression levels of N-cadherin,Vimentin were increased,and the protein and mRNA expression levels of E-cadherin were decreased in high glucose group(all P<0.01).Compared with the high glucose group,H2O2 levels were decreased in the high glucose+CAT group,24 h cell migration rate,number of invading cells,the expression levels of p-ERK,p-NF-κB proteins and the protein and mRNA expression levels of N-cadherin,Vimentin were decreased,the protein and mRNA expression levels of E-cadherin were increased in the high glucose+CAT and high glucose+PD98059 groups(all P<0.01).Conclusion:High glucose can induce H2O2 production and activate downstream ERK/NF-κB signaling pathway in ovarian cancer cells,promote invasion and migration of ovarian cancer cells,and affect the expression of epithelial-mesenchymal transition(EMT)-related factors in ovarian cancer cells.

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备注/Memo

备注/Memo:
基金项目:河南省医学科技攻关计划项目(LHGJ20230464)
更新日期/Last Update: 2024-08-08