[1]贾春美,郭 凯,杜天宇,等.肺浸润性腺癌中Prominin2表达特征及对细胞增殖和凋亡调控的作用研究[J].陕西医学杂志,2024,(7):991-995,999.[doi:DOI:10.3969/j.issn.1000-7377.2024.07.028]
　JIA Chunmei,GUO Kai,DU Tianyu,et al.Expression of Prominin2 and its regulation of cell proliferation and apoptosis in lung invasive adenocarcinoma[J].,2024,(7):991-995,999.[doi:DOI:10.3969/j.issn.1000-7377.2024.07.028]
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肺浸润性腺癌中Prominin2表达特征及对细胞增殖和凋亡调控的作用研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年7期
页码:
991-995,999
栏目:
临床病理
出版日期:
2024-07-05

文章信息/Info

Title:
Expression of Prominin2 and its regulation of cell proliferation and apoptosis in lung invasive adenocarcinoma
作者:
贾春美; 郭 凯; 杜天宇; 徐佳音
(锦州医科大学附属第一医院,辽宁 锦州 121000)
Author(s):
JIA Chunmei; GUO Kai; DU Tianyu; XU Jiayin
(The First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,China)
关键词:
肺;  浸润性腺癌;  Prominin2;  生物学行为;  增殖;  凋亡
Keywords:
Lung;  Invasive adenocarcinoma;  Prominin2;  Biological behavior;  Proliferation;  Apoptosis
分类号:
R 734.2
DOI:
DOI:10.3969/j.issn.1000-7377.2024.07.028
文献标志码:
A
摘要:
目的:检测Prominin2(PROM2)在肺浸润性腺癌中的表达特征,分析其临床意义,探讨其对细胞增殖和凋亡的调控作用。方法:应用数据库进行生物信息分析预测PROM2在肺浸润性腺癌和正常肺组织中的表达差异。选择肺浸润性腺癌行手术治疗的患者58例,留取术后肿瘤组织和周围正常肺组织。免疫组化法检测PROM2、增殖细胞核抗原(PCNA)、Bcl-2相关X蛋白(BAX)、B细胞淋巴瘤2(Bcl-2)和半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)的表达。选择人肺腺癌细胞系A549和人永生化肺上皮细胞系16HBE,合成小干扰RNA-PROM2并转染A549细胞系建立si-RNA-PROM2(si-PROM2)组,并设立空载体转染组(si-NC)和空白对照组(NC),应用Western blot法检测PROM2,PCNA、BAX、Bcl-2和Caspase-3的表达,CCK-8实验观察细胞增殖活性,流式细胞术检测细胞凋亡率。结果:GEPIA数据库预测到PROM2在肺腺癌中的表达可能高于正常肺组织。PROM2在肺浸润性腺癌中表达的阳性率明显高于癌旁正常肺组织, PROM2在肺浸润性腺癌不同肿瘤最大径、有无脏层胸膜累犯的表达中差异有统计学意义(均P<0.05),而在不同性别、年龄、有无淋巴结转移、不同病理类型的表达中差异无统计学意义(均P>0.05)。肺浸润性腺癌中PROM2与PCNA、PROM2与Bcl-2均具有正相关性,PROM2与BAX、PROM2与Caspase-3均具有负相关性。肺腺癌细胞系A549中PROM2的表达量明显高于人永生化肺上皮细胞系。A549的si-PROM2组中PCNA和Bcl-2的表达明显低于si-NC组和NC组,BAX和Caspase-3的表达明显高于si-NC组和NC组。si-PROM2组在48 h和72 h的细胞增殖活性明显低于si-NC组和NC组,si-PROM2组细胞凋亡率明显高于si-NC组和NC组。结论:肺浸润性腺癌中PROM2的表达升高,参与病变的形成和进展,PROM2可能通过促进细胞增殖、抑制凋亡发挥生物学作用。
Abstract:
Objective:To detect the expression of Prominin2(PROM2)in lung invasive adenocarcinoma,analyze its clinical significance,and explore its regulation of cell proliferation and apoptosis.Methods:Bioinformatic analysis was applied to predict the difference of PROM2 expression in lung invasive adenocarcinoma and normal lung tissues.Fifty-eight patients diagnosed with lung invasive adenocarcinoma were selected,and postoperative tumor tissues and surrounding normal lung tissues were collected.Expressions of PROM2,proliferating cell nuclear antigen(PCNA),Bcl-2 associated X protein(BAX),B-cell lymphoma/leukemia-2(Bcl-2)and Caspase-3 were detected by immunohistochemistry.Human lung adenocarcinoma cell line A549 and human immortalized lung epithelial cell line 16HBE were selected.Human lung adenocarcinoma cell line A549 and human immortalized lung epithelial cell line 16HBE were selected.Small interfering RNA-PROM2 was synthesized and transfected into A549 cell line to establish si-RNA-PROM2(si-PROM2)group,and empty vector transfection group(si-NC)and blank control group(NC)were set up.The expressions of PROM2,PCNA,BAX,Bcl-2 and Caspase-3 were examined by Western blot,and the cell proliferation activity was detected by CCK-8 assay,and the apoptosis rate was detected by flow cytometry.Results:GEPIA database predicts that the expression of PROM2 in lung adenocarcinoma may be higher than that in normal lung tissue.The positive rate of PROM2 was significantly higher in lung invasive adenocarcinoma than that in normal lung tissues(P<0.05).The expression of PROM2 was statistically different in different maximum tumor diameter and with or without dirty pleural involvement in lung invasive adenocarcinoma(all P<0.05).The expression of PROM2 was not statistically different in sexes,ages,lymph node metastases and pathological types(all P>0.05).There was a positive correlation between PROM2 and PCNA,PROM2 and Bcl-2,and a negative correlation between PROM2 and BAX,PROM2 and Caspase-3 in lung invasive adenocarcinoma(all P<0.05).The expression of PROM2 was significantly higher in lung adenocarcinoma cell line A549 than that in human immortalized lung epithelial cell line(P<0.05).The expressions of PCNA and Bcl-2 were significantly lower in the si-PROM2 group than those in the si-NC and NC groups,and the expressions of BAX and Caspase-3 were significantly higher than those in the si-NC and NC groups on A549(all P<0.05).The proliferative activity was significantly lower in the si-PROM2 group than that in the si-NC and NC groups at 48 and 72 hours,and the apoptosis rate was significantly higher in the si-PROM2 group than that in the si-NC and NC groups(all P<0.05).Conclusion:The expression of PROM2 was elevated in lung invasive adenocarcinoma,which is involved in the formation and progression of lesions.PROM2 may play a biological role by promoting cell proliferation and inhibiting apoptosis.

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备注/Memo

备注/Memo:
基金项目:辽宁省锦州市科技计划项目( JZ2023B040)
更新日期/Last Update: 2024-07-04