[1]李世平,范平平,吝新英.糖尿病酮症酸中毒合并感染患者血清微小RNA-146a和微小RNA-155水平检测及意义[J].陕西医学杂志,2024,(6):828-831,837.[doi:DOI:10.3969/j.issn.1000-7377.2024.06.023]
　LI Shiping,FAN Pingping,LIN Xinying.Detection and significance of serum miR-146a and miR-155 levels in patients with diabetic ketoacidosis complicated with infection[J].,2024,(6):828-831,837.[doi:DOI:10.3969/j.issn.1000-7377.2024.06.023]
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糖尿病酮症酸中毒合并感染患者血清微小RNA-146a和微小RNA-155水平检测及意义
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年6期
页码:
828-831,837
栏目:
临床检验
出版日期:
2024-06-05

文章信息/Info

Title:
Detection and significance of serum miR-146a and miR-155 levels in patients with diabetic ketoacidosis complicated with infection
作者:
李世平; 范平平; 吝新英
(张家口市第一医院急诊重症监护室,河北 张家口 075000)
Author(s):
LI Shiping; FAN Pingping; LIN Xinying
(Emergency Intensive Care Unit,the First Hospital of Zhangjiakou,Zhangjiakou 075000,China)
关键词:
糖尿病酮症酸中毒;  感染;  微小RNA-146a;  微小RNA-155;  白细胞计数;  C-反应蛋白
Keywords:
Diabetes ketoacidosis;  Infection;  microRNA-146a;  microRNA-155;  white blood cell count;  C-reactive protein
分类号:
R 587.2
DOI:
DOI:10.3969/j.issn.1000-7377.2024.06.023
文献标志码:
A
摘要:
目的:检测糖尿病酮症酸中毒合并感染患者血清微小RNA(miR)-146a和miR-155水平,分析其意义。方法:选取DKA合并感染患者76例(感染组)和单纯DKA未合并感染患者60例(未感染组)。入院后24 h内,对实验室生化指标进行检测,采用实时荧光定量聚合酶链反应(RT-qPCR)测定血清miR-146a、miR-155水平。分析DKA合并感染的影响因素, DKA患者血清miR-146a、miR-155、白细胞计数(WBC)和C-反应蛋白(CRP)的相关性,以及血清miR-146a、miR-155对DKA合并感染的诊断价值。结果:感染组WBC、CRP以及血清miR-146a 、miR-155水平高于未感染组(均P<0.05)。WBC、CRP、miR-146a、miR-155是 DKA患者合并感染的独立危险因素(均P<0.05)。DKA合并感染患者血清miR-146a、miR-155与WBC、CRP呈正相关,且血清miR-146a与miR-155呈正相关(均P<0.05)。血清miR-146a和miR-155对DKA合并感染均有一定的诊断价值,且两项联合的诊断价值更高(均P<0.05)。结论:DKA合并感染患者血清miR-146a、miR-155呈高水平,与WBC、CRP密切相关,对DKA合并感染具有较高诊断价值。
Abstract:
Objective:To detect serum microRNA(miR)-146a and miR-155 levels in patients with diabetic ketoacidosis(DKA)complicated with infection,and analyze their significance.Methods:A total of 76 DKA patients with infection(infection group)and 60 DKA patients without infection(uninfected group)were enrolled.Within 24 hours after admission,laboratory biochemical indicators were detected,and the serum levels of miR-146a and miR-155 were determined by RT-qPCR.The influencing factors of DKA complicated with infection,the correlation of serum miR-146a,miR-155,white blood cell count(WBC)and C-reactive protein(CRP)in DKA patients,and the diagnostic value of serum miR-146a and miR-155 for DKA complicated with infection were analyzed.Results: The levels of WBC,CRP,and serum miR-146a and miR-155 in the infection group were higher than those in the non-infection group(all P<0.05).WBC,CRP,miR-146a and miR-155 were independent risk factors for DKA complicated with infection(all P<0.05).Serum miR-146a and miR-155 were positively correlated with WBC and CRP,and serum miR-146a was positively correlated with miR-155 in DKA patients with infection(all P<0.05).Both serum miR-146a and miR-155 had certain diagnostic value for DKA complicated with infection,and the combination of the two had a higher diagnostic value(all P<0.05).Conclusion:The serum levels of miR-146a and miR-155 in DKA patients complicated with infection are high and closely related to WBC and CRP,which have high diagnostic value for DKA complicated with infection.

参考文献/References:

[1] 张俊,张立文,李和平.血清高敏C反应蛋白和免疫球蛋白E检测在糖尿病酮症酸中毒电解质紊乱中的应用价值[J].陕西医学杂志,2022,51(6):737-740.
[2] PIKIELNY P R,LURIE T H,RABIA R,et al.Hospitalization of patients with diabetes due to ketoacidosis before and after the initiation of sodium-glucose cotransporter-2 inhibitors[J].Endocr Pract,2023,29(9):686-691.
[3] AHMAD R,NARWARIA M,SINGH A,et al.Detecting diabetic ketoacidosis with infection:Combating a life-threatening emergency with practical diagnostic tools[J].Diagnostics(Basel),2023,13(14):2441-2455.
[4] HUANG H,ZHU J,GU L,et al.TLR7 mediates acute respiratory distress syndrome in sepsis by sensing extracellular miR-146a[J].Am J Respir Cell Mol Biol,2022,67(3):375-388.
[5] CHENG Y,TANG Q,LI Y,et al.The polymorphisms of miR-146a SNPs are associated with asthma in Southern Chinese Han population[J].Gene,2023,879:147587.
[6] ALIJANI E,RAD F R,KATEBI A,et al.Differential expression of miR-146 and miR-155 in active and latent tuberculosis infection[J].Iran J Public Health,2023,52(8):1749-1757.
[7] 王宏伟,宋晓萍,裴复阳.呼吸机相关肺炎患者病原菌分布及血清miR-146a、miR-155的检测分析[J].热带医学杂志,2022,22(1):37-40.
[8] 中华医学会糖尿病学分会.中国高血糖危象诊断与治疗指南[J].中华糖尿病杂志,2013,5(8):449-461.
[9] HURISSI E,ALAMEER M,AGEELI F,et al.The association between SARS-CoV-2 infection and diabetic ketoacidosis in patients with new-onset diabetes:A retrospective study from a diabetic center in Saudi Arabia[J].Pediatr Rep,2022,14(4):519-527.
[10] ISHIMARU N,SHIMOKAWA T,NAKAJIMA T,et al.Bacteremia in patients with diabetic ketoacidosis:A cross-sectional study[J].Hosp Pract(1995),2023,51(2):95-100.
[11] VITALE R J,VALTIS Y K,MCDONNELL M E,et al.Euglycemic diabetic ketoacidosis with COVID-19 infection in patients with type 2 diabetes taking SGLT2 inhibitors[J].AACE Clin Case Rep,2021,7(1):10-13.
[12] 刁冰冰,梁群.化纤胶囊Ⅱ号方治疗脓毒症急性肺损伤疗效及对患者血管内皮细胞功能、毛细血管渗漏指数的影响[J].陕西中医,2023,44(1):45-49,59.
[13] PENG X,HE F,MAO Y,et al.MiR-146a promotes M2 macrophage polarization and accelerates diabetic wound healing by inhibiting the TLR4/NF-κB axis[J].J Mol Endocrinol,2022,69(2):315-327.
[14] ALBELTAGY R S,MUMTAZ F,ABDEL MONEIM A E,et al.N-Acetylcysteine reduces miR-146a and NF-κB p65 inflammatory signaling following cadmium hepatotoxicity in rats[J].Biol Trace Elem Res,2021,199(12):4657-4665.
[15] CHU T,XU X,RUAN Z,et al.MiR-146a contributes to atherosclerotic plaque stability by regulating the expression of TRAF6 and IRAK-1[J].Mol Biol Rep,2022,49(6):4205-4216.
[16] HSIEH Y T,CHOU Y C,KUO P Y,et al.Down-regulated miR-146a expression with increased neutrophil extracellular traps and apoptosis formation in autoimmune-mediated diffuse alveolar hemorrhage[J].J Biomed Sci,2022,29(1):62-81.
[17] 李晓滨,白建云,赵启兵.右美托咪定对心肌缺血再灌注损伤大鼠的作用及机制研究[J].陕西医学杂志,2022,51(9):1060-1065.
[18] 张越琳,曹家铭,刘惠媛,等.miR-146a和miR-499多态性与类风湿性关节炎易感性的Meta分析[J].中华临床医师杂志:电子版,2021,15(6):418-428.
[19] TONG J,DUAN Z,ZENG R,et al.MiR-146a negatively regulates aspergillus fumigatus-induced TNF-α and IL-6 secretion in THP-1 macrophages[J].Mycopathologia,2021,186(3):341-354.
[20] KARIMI M,MOHAMMADNIA A,AMINI M A,et al.Overexpression of miR-146a and miR-155 are potentially biomarkers and predict unfavorable relationship between gastric cancer and helicobacter pylori infection[J].Chonnam Med J,2023,59(3):167-173.
[21] XU W D,FENG S Y,HUANG A F.Role of miR-155 in inflammatory autoimmune diseases:A comprehensive review[J].Inflamm Res,2022,71(12):1501-1517.
[22] 张驰,李奥博,王心怡,等.温阳振衰颗粒调控miR-155/p38MAPK途径减轻大鼠心肌缺血再灌注损伤机制研究[J].陕西中医,2023,44(8):1005-1009.
[23] 谭仕廉,李艳丽,何永勋,等.miR-155靶向JAK/STAT3通路促进LPS诱导的THP-1巨噬细胞炎性反应[J].医学研究杂志,2022,51(4):41-45.
[24] FEI Y,WANG Z,HUANG M,et al.MiR-155 regulates M2 polarization of hepatitis B virus-infected tumour-associated macrophages which in turn regulates the malignant progression of hepatocellular carcinoma[J].J Viral Hepat,2023,30(5):417-426.
[25] LI B,GUO L,HE Y,et al.MicroRNA-155 expression is associated with pulpitis progression by targeting SHIP1[J].Mol Biol Rep,2022,49(9):8575-8586.

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备注/Memo

备注/Memo:
基金项目:河北省医学科学研究课题(20221601); 河北省张家口市重点研发计划项目(2221228D)
更新日期/Last Update: 2024-06-05