[1]易三凤,屈银宗,龚承先,等.环状RNA GRIN2B通过调控微小RNA-135b对食管癌细胞增殖和侵袭的影响实验研究[J].陕西医学杂志,2024,(3):308-312.[doi:DOI:10.3969/j.issn.1000-7377.2024.03.004]
　YI Sanfeng,QU Yinzong,GONG Chengxian,et al.Effects of circular RNA GRIN2B on proliferation and invasion of esophageal cancer cells through regulation of microRNA-135b[J].,2024,(3):308-312.[doi:DOI:10.3969/j.issn.1000-7377.2024.03.004]
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环状RNA GRIN2B通过调控微小RNA-135b对食管癌细胞增殖和侵袭的影响实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年3期
页码:
308-312
栏目:
基础研究
出版日期:
2024-03-05

文章信息/Info

Title:
Effects of circular RNA GRIN2B on proliferation and invasion of esophageal cancer cells through regulation of microRNA-135b
作者:
易三凤1; 屈银宗1; 龚承先1; 赵艳洁2; 杨建美1
(1.湖北省中西医结合医院消化内科,湖北 武汉 430015; 2.青岛大学附属医院肿瘤科,山东 青岛 266003)
Author(s):
YI Sanfeng; QU Yinzong; GONG Chengxian; ZHAO Yanjie; YANG Jianmei
(Department of Gastroenterology,Hubei Provincial Hospital of Integrated Chinese & Western Medicine,Wuhan 430015,China)
关键词:
食管癌;  环状RNA GRIN2B;  微小RNA-135b;  细胞增殖;  细胞侵袭;  机制
Keywords:
Esophageal carcinoma;  Circular RNA GRIN2B;  microRNA-135b;  Cell proliferation;  Cell invasion;  Mechanism
分类号:
R 73-3
DOI:
DOI:10.3969/j.issn.1000-7377.2024.03.004
文献标志码:
A
摘要:
目的:探讨环状RNA GRIN2B(circ-GRIN2B)对食管癌细胞增殖和侵袭的影响及其分子机制。方法:采用RT-qPCR检测circ-GRIN2B在食管癌细胞株Eca109、TE-13、KYSE30、OE33、EC9706和正常食管上皮细胞HET-1A中的表达,筛选circ-GRIN2B表达最低的细胞株进行后续实验。在食管癌细胞中转染circ-GRIN2B过表达质粒(circ-GRIN2B组)和空载对照质粒(NC组)。采用平板克隆实验和Transwell实验检测circ-GRIN2B对食管癌细胞增殖和侵袭的影响。采用circRNADb软件预测circ-GRIN2B与微小RNA-135b(miR-135b)的结合位点并用双荧光素酶报告基因验证两者的靶向关系。采用RT-qPCR检测两组miR-135b表达。采用Western blot检测细胞增殖相关蛋白[周期蛋白依赖性激酶8(CDK8)、周期素C(Cyclin C)]和侵袭相关蛋白[纤维结合蛋白(FN)、转录因子8(ZLF8)、叉头盒蛋白C2(FOXC2)]表达。结果:与HET-1A细胞比较,circ-GRIN2B在细胞株Eca109、TE-13、KYSE30、OE33、EC9706表达降低,且在EC9706细胞中表达量最低(均P<0.05)。circ-GRIN2B组circ-GRIN2B表达量高于NC组(P<0.01)。circ-GRIN2B组EC9706细胞集落数量少于NC组(P<0.01)。circ-GRIN2B组EC9706细胞侵袭数目小于NC组(P<0.01)。miR-135b是circ-GRIN2B的靶基因。circ-GRIN2B组miR-135b表达量低于NC组(P<0.01)。circ-GRIN2B组EC9706细胞增殖相关蛋白和侵袭相关蛋白表达水平较NC组降低(均P<0.05)。结论:circ-GRIN2B在食管癌细胞中低表达,其可能通过下调miR-135b表达抑制食管癌细胞的增殖和侵袭。
Abstract:
Objective:To investigate the effects of circular RNA GRIN2B(circ-GRIN2B)on the proliferation and invasion of esophageal carcinoma cells and its molecular mechanism.Methods:The expression of circ-GRIN2B in esophageal cancer cell lines Eca109,TE-13,KYSE30,OE33,EC9706 and normal esophageal epithelial cells HET-1A was detected by RT-qPCR,and the lowest expression cell of circ-GRIN2B was screened for follow-up experiments.Circ-GRIN2B overexpression plasmid(circ-GRIN2B group)and no-load control plasmid(NC group)were transfected into esophageal cancer cells.The effects of circ-GRIN2B on the proliferation and invasion of esophageal carcinoma cells were examined by plate cloning assay and Transwell assay.CircRNADb software was used to predict the binding sites of circ-GRIN2B and miR-135b,and dual luciferase reporter gene was used to verify the targeting relationship between the two.The expression of miR-135b was detected by RT-qPCR.The expressions of proliferation-related proteins(Cyclin dependent kinase 8 [CDK8],Cyclin C)and invasion-related proteins(Fibronection [FN],transcription factor 8 [ZLF8],forkhead box protein C2 [FOXC2])were detected by Western blot.Results:Compared with HET-1A cells,the expressions of circ-GRIN2B were decreased in Eca109,TE-13,KYSE30,OE33 and EC9706 cells,and the expression level was lowest in EC9706 cells(all P<0.05).The expression of circ-GRIN2B in circ-GRIN2B group was higher than that in NC group(P<0.01).The number of EC9706 cell colonies in circ-GRIN2B group was lower than that in NC group(P<0.01).The number of EC9706 cells invaded in circ-GRIN2B group was less than that in NC group(P<0.01).MiR-135b was a target gene for circ-GRIN2B.The expression level of miR-135b in circ-GRIN2B group was lower than that in NC group(P<0.01).The expression levels of proliferation-related proteins and invasion-related proteins in EC9706 cells in circ-GRIN2B group were lower than those in NC group(all P<0.05).Conclusion:Circ-GRIN2B is underexpressed in esophageal cancer cells,and it may inhibit the proliferation and invasion of esophageal cancer cells by down-regulating the expression of miR-135b.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81802415)
更新日期/Last Update: 2024-03-05