[1]刘子歌,袁 昂,王文鹏,等.姜黄素对磨损颗粒诱导的体外破骨细胞的作用及机制实验研究[J].陕西医学杂志,2024,(3):291-296,302.[doi:DOI:10.3969/j.issn.1000-7377.2024.03.001]
　LIU Zige,YUAN Ang,WANG Wenpeng,et al.Effect and mechanism of curcumin on abrasive particles-induced osteoclasts in vitro[J].,2024,(3):291-296,302.[doi:DOI:10.3969/j.issn.1000-7377.2024.03.001]
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姜黄素对磨损颗粒诱导的体外破骨细胞的作用及机制实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年3期
页码:
291-296,302
栏目:
基础研究
出版日期:
2024-03-05

文章信息/Info

Title:
Effect and mechanism of curcumin on abrasive particles-induced osteoclasts in vitro
作者:
刘子歌1; 袁 昂2; 王文鹏2; 陈德胜2
(1.广西医科大学临床医学院,广西 南宁 530020; 2.宁夏回族自治区人民医院骨科,宁夏 银川 750002)
Author(s):
LIU Zige; YUAN Ang; WANG Wenpeng; CHEN Desheng
(School of Clinical Medicine,Guangxi Medical University,Nanning 530020,China)
关键词:
姜黄素;  磨损颗粒;  核因子-κB信号通路;  破骨细胞;  巨噬细胞
Keywords:
Curcumin;  Abrasive particles;  Nuclear factor kappaB signaling pathway;  Osteoclasts;  Macrophages
分类号:
R 285
DOI:
DOI:10.3969/j.issn.1000-7377.2024.03.001
文献标志码:
A
摘要:
目的:探讨姜黄素(Cur)对磨损颗粒诱导的体外破骨细胞的作用及机制。方法:将小鼠RAW264.7巨噬细胞分为五组培养,即空白组(完全培养基)、模型组[空白组+钛(Ti)颗粒 0.1 mg/ml]、低浓度Cur组(模型组+Cur 1 μmol/L)、中浓度Cur组(模型组+Cur 10 μmol/L)和高浓度Cur组(模型组+Cur 15 μmol/L)。CCK-8法检测细胞增殖活性。抗酒石酸酸性磷酸酶(TRAP)染色法和鬼笔环肽染色法检测成熟破骨细胞数量。酶联免疫吸附(ELISA)法检测细胞上清液中肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β和基质金属蛋白酶(MMP)-9的表达。Western blot法检测p-p65、磷酸化核因子(NF)-κB抑制蛋白α(p-IκBα)蛋白水平。PCR法检TNF-α、IL-1β、活化T细胞核因子(NFATc)1和TRAP mRNA的表达水平。结果:Cur浓度小于15 μmol/L对RAW264.7巨噬细胞增殖活性的影响与空白组比较差异无统计学意义(均P>0.05),因此采用不高于15 μmol/L Cur进行后续实验。中、高浓度Cur组TRAP阳性成熟破骨细胞数量低于模型组(均P<0.05)。与模型组比较,不同浓度Cur组TNF-α、IL-1β和MMP-9表达,p-p65与p-IκBα蛋白表达,TRAP、TNF-α、IL-1β和NFATc1 mRNA表达降低(均P<0.05)。结论:Cur对体外磨损颗粒诱导的破骨细胞生成和破骨细胞功能有抑制作用,其机制可能是抑制NF-κB信号通路。
Abstract:
Objective:To investigate the effect and mechanism of curcumin(Cur)on abrasive particles-induced osteoclasts in vitro.Methods:Mouse RAW264.7 macrophages were divided into five groups for cultivation:blank group(complete medium),model group(blank group+Ti particles 0.1 mg/ml),low concentration Cur group(model group+Cur 1 μmol/L),medium concentration Cur group(model group+Cur 10 μmol/L)and high concentration Cur group(model group+Cur 15 μmol/L).Cell proliferation activity was detected by CCK-8 assay.The number of mature osteoclasts was detected by TRAP staining and phalloidin staining.The expression of TNF-α,IL-1β and MMP-9 in cell supernatant was detected by ELISA.The protein levels of p-p65 and NF-κB inhibitor protein α(p-IκBα)were detected by Western blot.The mRNA expression levels of TNF-α,IL-1β,NFATc1 and TRAP were detected by PCR.Results:The effect of Cur at a concentration of less than 15 μmol/L on the proliferation activity of RAW264.7 macrophages was not statistically different from that of the blank group(all P>0.05),so 15 μmol/L Cur was used for the subsequent experiments.The number of TRAP positive mature osteoclasts in the medium and high concentration Cur groups was lower than that in the model group(all P<0.05).Compared with the model group,the expressions of TNF-α,IL-1β and MMP-9,p-p65 and p-IκBα protein,TRAP,TNF-α,IL-1β and NFATc1 mRNA were decreased in the different concentrations of Cur groups(all P<0.05).Conclusion:Cur can inhibit abrasive particles-induced osteoclastogenesis and osteoclast function in vitro possibly by inhibiting the NF-κB signaling pathway.

参考文献/References:

[1] KOKS S,WOOD D J,REIMANN E,et al.The genetic variations associated with time to aseptic loosening after total joint arthroplasty[J].J Arthroplasty,2020,35(4):981-988.
[2] BRESSAN E,FERRONI L,GARDIN C,et al.Metal nanoparticles released from dental implant surfaces:Potential contribution to chronic inflammation and peri-implant bone loss[J].Materials(Basel),2019,12(12):2036.
[3] LIU X,QU X,WU C,et al.The effect of enoxacin on osteoclastogenesis and reduction of titanium particle-induced osteolysis via suppression of JNK signaling pathway[J].Biomaterials,2014,35(22):5721-5730.
[4] SHIN D,KIM M,LEE S,et al.Inhibitory effects of luteolin on titanium particle-induced osteolysis in a mouse model[J].Acta Biomaterialia,2012,8(9):3524-3531.
[5] PING Z,WANG Z,SHI J,et al.Inhibitory effects of melatonin on titanium particle-induced inflammatory bone resorption and osteoclastogenesis via suppression of NF-κB signaling[J].Acta Biomaterialia,2017,62:362-371.
[6] 周婷婷,谭劲,吴丹,等.姜黄素对人口腔黏膜上皮细胞系增殖及Wnt/β-catenin信号通路的影响[J].陕西中医,2022,43(9):1155-1158.
[7] 刘子歌,张晨,宋国瑞,等.Cur对钛颗粒诱导的小鼠气囊植骨模型溶骨的保护作用[J].中成药,2022,44(7):2295-2299.
[8] CHEN W,LI Z,GUO Y,et al.Wear particles promote reactive oxygen species-mediated inflammation via the nicotinamide adenine dinucleotide phosphate oxidase pathway in macrophages surrounding loosened implants[J].Cellular Physiology and Biochemistry,2015,35(5):1857-1867.
[9] 张续,郑洁,赵莉平,等.独活寄生汤对膝骨关节炎大鼠IL-17/NF-κB通路的影响[J].陕西中医,2022,43(6):691-695.
[10] ORMSBY R T,CANTLEY M,KOGAWA M,et al.Evidence that osteocyte perilacunar remodelling contributes to polyethylene wear particle induced osteolysis[J].Acta Biomaterialia,2016,33:242-251.
[11] CHAI L,ZHOU K,WANG S,et al.Psoralen and bakuchiol ameliorate M-CSF plus RANKL-induced osteoclast differentiation and bone resorption via inhibition of AKT and AP-1 pathways in vitro[J].Cellular Physiology and Biochemistry,2018,48(5):2123-2133.
[12] MEMARZIA A,KHAZDAIR M R,BEHROUZ S,et al.Experimental and clinical reports on anti-inflammatory,antioxidant,and immunomodulatory effects of curcuma longa and curcumin,an updated and comprehensive review[J].Biofactors,2021,47(3):311-350.
[13] SHIMIZU K,FUNAMOTO M,SUNAGAWA Y,et al.Anti-inflammatory action of curcumin and its use in the treatment of lifestyle-related diseases[J].European Cardiology Review,2019,14(2):117-122.
[14] ZHANG J,ZHENG Y,LUO Y,et al.Curcumin inhibits LPS-induced neuroinflammation by promoting microglial M2 polarization via TREM2/TLR4/NF-κB pathways in BV2 cells[J].Molecular Immunology,2019,116:29-37.
[15] YANG B,ZHOU H,ZHANG X,et al.Effect of radiation on the expression of osteoclast marker genes in RAW264.7 cells[J].Molecular Medicine Reports,2012,5(4):955-958.
[16] LI L,SAPKOTA M,GAO M,et al.Macrolactin F inhibits RANKL-mediated osteoclastogenesis by suppressing Akt,MAPK and NFATc1 pathways and promotes osteoblastogenesis through a BMP-2/smad/Akt/Runx2 signaling pathway[J].European Journal of Pharmacology,2017,815:202-209.
[17] BLASCHKE M,KOEPP R,CORTIS J,et al.IL-6,IL-1β,and TNF-α only in combination influence the osteoporotic phenotype in Crohn's patients via bone formation and bone resorption[J].Adv Clin Exp Med,2018,27(1):45-56.
[18] ZHAO Q,WANG X,LIU Y,et al.NFATc1:Functions in osteoclasts[J].Int J Biochem Cell Bio,2010,42(5):576-579.
[19] KIRKBY N S,CHAN M V,ZAISS A K,et al.Systematic study of constitutive cyclooxygenase-2 expression:Role of NF-κB and NFAT transcriptional pathways[J].Proceedings of the National Academy of Sciences,2016,113(2):434-439.
[20] SUNDARAM K,NISHIMURA R,SENN J,et al.RANK ligand signaling modulates the matrix metalloproteinase-9 gene expression during osteoclast differentiation[J].Experimental Cell Research,2007,313(1):168-178.
[21] 魏东梅,邵龙刚.芒柄花黄素对脓毒症小鼠炎性反应及MAPK/NF-κB通路影响的实验研究[J].陕西医学杂志,2023,52(5):503-507.
[22] ZHONG Z,ZHANG C,NI S,et al.NFATc1-mediated expression of SLC7A11 drives sensitivity to TXNRD1 inhibitors in osteoclast precursors[J].Redox Biol,2023,63:102711.
[23] 昌震,李大同,杨小彬,等.奇壬醇对骨质疏松症的治疗作用及机制研究进展[J].陕西医学杂志,2022,51(9):1167-1170.
[24] LI X,SHI J,TENG Y,et al.The preventative effect of baihe gujin pill on cisplatin-induced acute kidney injury by activating the PI3K/AKT and suppressing the NF-κB/MAPK pathways[J].J Ethnopharmacol,2024,318(Pt B):117071.
[25] NG C W,CHAN B C L,KO C H,et al.Human mast cells induce osteoclastogenesis through cell surface RANKL[J].Inflamm Res,2022,71(10-11):1261-1270.
[26] LIN X,ZHANG H,LIU J,et al.Aged callus skeletal stem/progenitor cells contain an inflammatory osteogenic population with increased IRF and NF-κB pathways and reduced osteogenic potential[J].Front Mol Biosci,2022,9:806528.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(82060408); 宁夏回族自治区重点研发计划项目(2021BEG03049); 宁夏回族自治区留学人员创新创业项目(2020-75); 宁夏回族自治区高等学校一流学科建设项目(NXYLXK2017A05)
更新日期/Last Update: 2024-03-05