[1]王雅清,王 勇,刘永胜.芦丁调控miR-155和HMGB1/RAGE通路对糖尿病视网膜病变大鼠炎性反应的影响[J].陕西医学杂志,2024,(2):184-188.[doi:DOI:10.3969/j.issn.1000-7377.2024.02.008]
　WANG Yaqing,WANG Yong,LIU Yongsheng.Effect of rutin on inflammatory response in diabetic retinopathy rats by regulating miR-155 and HMGB1/RAGE pathway[J].,2024,(2):184-188.[doi:DOI:10.3969/j.issn.1000-7377.2024.02.008]
点击复制

芦丁调控miR-155和HMGB1/RAGE通路对糖尿病视网膜病变大鼠炎性反应的影响
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年2期
页码:
184-188
栏目:
基础研究
出版日期:
2024-02-05

文章信息/Info

Title:
Effect of rutin on inflammatory response in diabetic retinopathy rats by regulating miR-155 and HMGB1/RAGE pathway
作者:
王雅清; 王 勇; 刘永胜
(保定市第二医院内分泌科,河北 保定071051)
Author(s):
WANG Yaqing; WANG Yong; LIU Yongsheng
(Department of Endocrinology,the No.2 Hospital of Baoding,Baoding 071051,China)
关键词:
糖尿病视网膜病变;  芦丁;  微小RNA-155;  高迁移率族蛋白1;  晚期糖基化终产物受体;  炎性反应
Keywords:
Diabetesretinopathy;  Rutin;  miRNA-155;  High mobility group protein 1;  Advanced glycosylation end-product receptor;  Inflammatory reaction
分类号:
R 774
DOI:
DOI:10.3969/j.issn.1000-7377.2024.02.008
文献标志码:
A
摘要:
目的:探讨芦丁对糖尿病视网膜病变大鼠炎性反应的影响及其机制。方法:采用腹腔注射链脲佐菌素的方法制备糖尿病大鼠模型,将造模成功的大鼠随机分为模型组(0.9%氯化钠溶液灌胃)和芦丁低、中、高剂量组[分别以50、100、150 mg/(kg·d)芦丁灌胃],并取正常大鼠作为对照组(0.9%氯化钠溶液灌胃),每组20只,持续给药12周后,比较各组大鼠视网膜组织中伊凡斯兰渗透量、神经节细胞凋亡和微小RNA(miR)-155、 高迁移率族蛋白1(HMGB1)、晚期糖基化终产物受体(RAGE)mRNA表达水平,以及炎症因子水平。结果:对照组、模型组和芦丁低、中、高剂量组中伊凡斯兰渗透量分别为(1.61±0.31)μg/g、(12.84±1.24)μg/g、(9.85±0.62)μg/g、(6.80±0.53)μg/g、(2.87±0.51)μg/g,神经节细胞凋亡指数分别为(1.09±0.45)%、(30.96±2.50)%、(24.65±2.48)%、(18.61±1.41)%、(8.36±0.54)%,miR-155表达水平分别为(0.38±0.03)、(1.92±0.15)、(1.55±0.12)、(1.09±0.10)、(0.55±0.05),HMGB1 mRNA表达水平分别为(0.26±0.03)、(0.77±0.05)、(0.68±0.04)、(0.57±0.04)、(0.34±0.03),RAGE mRNA表达水平分别为(0.18±0.02)、(0.67±0.04)、(0.58±0.03)、(0.45±0.03)、(0.23±0.02)。模型组和对照组比较,伊凡斯兰渗透量、凋亡指数和miR-155、HMGB1、RAGE mRNA水平均升高(均P<0.05); 芦丁低、中、高剂量组中上述指标呈剂量依赖性降低(均P<0.05)。模型组、对照组与芦丁低、中、高剂量组,芦丁各组的炎症因子水平低于模型组(均P<0.05)。结论:芦丁可保护糖尿病大鼠视网膜损伤,延缓视网膜炎症效应,其作用机制可能与抑制miR-155和HMGB1/RAGE通路有关。
Abstract:
Objective:To investigate the effect of rutin on inflammatory response in rats with diabetic retinopathy and its mechanism.Methods:The diabetic rat model was established by intraperitoneal injection of streptozotocin.The successful diabetic rats were randomly divided into model group(intragastric administration of 0.9% sodium chloride solution),low-,medium- and high-dose rutin groups(intragastric administration of 50,100,and 150 mg/kg rutin,respectively),and normal rats were taken as control group(intragastric administration of 0.9% sodium chloride solution),with 20 rats in each group.After 12 weeks of continuous administration,the permeability of evans blue,the apoptosis of ganglion cells,the mRNA expression levels of miR-155,HMGB1,RAGE,and the levels of inflammatory factors in the retinal tissue of each group were compared.Results:In control group,model group and low,middle and high-dose rutin groups,the permeability of evans blue,were(1.61±0.31)μg/g,(12.84±1.24)μg/g,(9.85±0.62)μg/g,(6.80±0.53)μg/g,(2.87±0.51)μg/g,respectively; the ganglion cell apoptosis index were(1.09±0.45)%,(30.96±2.50)%,(24.65±2.48)%,(18.61±1.41)%,(8.36±0.54)%,respectively; the expression levels of miR-155 were(0.38±0.03),(1.92±0.15),(1.55±0.12),(1.09±0.10)and(0.55±0.05),respectively; HMGB1 mRNA expression levels were(0.26±0.03),(0.77±0.05),(0.68±0.04),(0.57±0.04),(0.34±0.03),respectively; the mRNA expression level of RAGE were(0.18±0.02),(0.67±0.04),(0.58±0.03),(0.45±0.03)and(0.23±0.02),respectively.Compared with the control group,the permeability of evans blue,apoptosis index and the mRNA levels of miR-155,HMGB1 and RAGE were increased in the model group(all P<0.05),and these parameters were decreased in a dose-dependent manner in the low-,medium- and high-dose rutin groups(all P<0.05).The levels of inflammatory factors in the model group,the control group and the low-,medium- and high-dose rutin groups were lower than those in the model group(all P<0.05).Conclusion:Rut can protect retinal damage and delay retinal inflammatory effect in diabetic rats,and its mechanism may be related to inhibition of miR-155 and HMGB1/RAGE pathway.

参考文献/References:

[1] 孟宏,王桂青,杜竹君.西红花苷通过调控TLR4/MyD88/NF-κB通路改善糖尿病大鼠视网膜病变机制研究[J].陕西医学杂志,2023,52(5):531-535,540.
[2] 邓立萍,谢孟君,祝子喻,等.葛根总黄酮提取物对糖尿病大鼠视网膜病变的影响分析[J].中药药理与临床,2021,37(2):54-59.
[3] 余婵娟,赵进东,杨迪,等.黄芪甲苷对糖尿病大鼠视网膜病变的防治作用及对氧化应激通路的影响[J].中华中医药学刊,2020,38(11):194-197.
[4] 孙建娟,吴光秀,令狐昌敏,等.参芪降糖颗粒治疗糖尿病周围神经病变临床研究[J].陕西中医,2021,42(7):893-895.
[5] 陈明霞,马雪,陈佩文,等.绿萝花黄酮对糖尿病周围神经病变小鼠运动神经传导速度及NF-κB信号通路的影响[J].陕西中医,2022,43(10):1347-1353.
[6] 兰婷,贾如.芦丁减轻LPS诱导的牙周膜干细胞炎症、成骨分化和成脂作用[J].山西医科大学学报,2022,53(7):878-886.
[7] 祁珊珊,何佳,龚帅,等.黑米花青苷对糖尿病大鼠视网膜病变的保护作用[J].中国临床药理学杂志,2020,36(22):3751-3754,3759.
[8] 赵艳霞,王锋,王爽.白蒺藜皂苷对2型糖尿病大鼠视网膜的保护作用及TrkB信号通路的影响[J].解放军医药杂志,2020,32(10):24-28.
[9] 徐辉勇,陈金鹏,章剑.柚皮素对糖尿病视网膜病变大鼠氧化损伤,细胞凋亡及NRF2-ARE的影响[J].临床和实验医学杂志,2020,19(2):128-132.
[10] 丁宇,赵殿臣,赏楠.黄芪保心汤对慢性心力衰竭模型大鼠心室重构及内质网应激凋亡的抑制作用[J].世界中医药,2021,16(18):2714-2718.
[11] 司长峰,卜荔,徐楠,等.柴胡皂苷D对糖尿病视网膜病变大鼠的治疗作用[J].眼科新进展,2021,41(10):925-929.
[12] 张铮,张建,胡久丽,等.氧化苦参碱对糖尿病视网膜病变大鼠视网膜Toll样受体4 mRNA和核转录因子κB mRNA表达的影响[J].河北中医,2019,41(5):735-738.
[13] 张云东,朱德才,赵彪,等.曲克芦丁联合拜阿司匹林治疗老年伴大脑中动脉高密度征急性脑梗死静脉溶栓患者的疗效[J].老年医学与保健,2021,27(4):790-793.
[14] 李洪君,张广丽.银杏二萜内酯葡胺注射液联合曲克芦丁脑蛋白水解物注射液治疗急性脑梗死的疗效观察[J].广西医学,2021,43(17):2062-2066.
[15] 吴毓聪,程亚楠,杨宾,等.芦丁对4NQO诱发大鼠舌癌的保护作用及炎症相关蛋白表达的影响[J].华南国防医学杂志,2021,35(2):79-83,115.
[16] 李铁军.4NQO诱发两种品系大鼠舌癌过程中p53蛋白的表达特点[J].中华口腔医学杂志,2000,35(2):126-128.
[17] 岳朝驰,杨向东,李俊,等.芦丁对溃疡性结肠炎小鼠的干预作用及NLRP3炎症小体的影响[J].现代中西医结合杂志,2021,30(10):1073-1078.
[18] WILLIS S,DAY C L,HINDS M G,et al.The Bcl-2-regulated apoptotic pathway[J].J Cell Sci,2003,116(20):4053-4056.
[19] PONDER K G,BOISE L H.The prodomain of caspase-3 regulates its own removal and caspase activation[J].Cell Death Discov,2019,28:56.
[20] 黎昌江,陈方安,洪娟,等.miR-155靶向Nrf2/HO-1通路对高糖作用下视网膜血管内皮细胞氧化应激损伤的影响[J].东南大学学报(医学版),2021,40(5):666-674.
[21] 邵娟,白淑玮,田蕴霖.高迁移率族蛋白B-1和NADPH氧化酶衍生活性氧对糖尿病视网膜病变大鼠视网膜电图波幅和玻璃体通透性的影响[J].陕西医学杂志,2022,51(5):515-519,524.
[22] SOLMAZ V,KAYA M,USLU F B,et al.Papaverine has therapeutic potential for sepsis-induced neuropathy in rats,possibly via the modulation of hmgb1-rage axis and its antioxidant prosperities[J].J Invest Surg,2020,25:1-7.
[23] 高啸,沈莹.HMGB1-RAGE/TLRs-NF-κB信号通路中关键蛋白的表达与糖尿病肾病的关系[J].转化医学杂志,2020,9(6):331-334,339.
[24] 秘乐,范绍辉,徐宇,等.HMGB1-TLR4/RAGE信号通路在ALI/ARDS中的研究进展[J].国际呼吸杂志,2021,41(9):703-708.
[25] 郭慕真.百草枯经血脑屏障刺激神经元释放HMGB1介导RAGE/NF-κB信号通路致炎症反应的机制[D].银川:宁夏医科大学,2019.

相似文献/References:

[1]薛晓辉,马庆祥.血清VEGF与SE-选择素在糖尿病视网膜病变患者中的表达及意义[J].陕西医学杂志,2016,(10):1316.
[2]郑锦标,马萍萍,马瑞倩.糖尿病伴发视网膜微血管异常临床特征及危险因素调查研究[J].陕西医学杂志,2019,(11):1571.
[3]张 乔,魏春艳,贺 敏△.自制防护袖套在玻璃体切除术后被迫体位中的应用价值[J].陕西医学杂志,2020,49(12):1648.[doi:DOI:10.3969/j.issn.1000-7377.2020.12.029]

备注/Memo

备注/Memo:
基金项目:河北省卫生健康委员会医学科学研究计划项目(20232040); 河北省保定市科学技术研究与发展计划项目(2141ZF008)
更新日期/Last Update: 2024-02-05