[1]齐淑静,付改霞,齐瑞霞.小檗碱调节HIF-1α/VEGF信号通路对皮肤溃疡大鼠血管生成和创面愈合的影响[J].陕西医学杂志,2024,(2):173-178.[doi:DOI:10.3969/j.issn.1000-7377.2024.02.006]
　QI Shujing,FU Gaixia,QI Ruixia.Impacts of berberine on angiogenesis and wound healing in skin ulcer rats by regulating HIF-1α/VEGF signaling pathway[J].,2024,(2):173-178.[doi:DOI:10.3969/j.issn.1000-7377.2024.02.006]
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小檗碱调节HIF-1α/VEGF信号通路对皮肤溃疡大鼠血管生成和创面愈合的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年2期
页码:
173-178
栏目:
基础研究
出版日期:
2024-02-05

文章信息/Info

Title:
Impacts of berberine on angiogenesis and wound healing in skin ulcer rats by regulating HIF-1α/VEGF signaling pathway
作者:
齐淑静; 付改霞; 齐瑞霞
(河北工程大学附属医院,河北 邯郸056001)
Author(s):
QI Shujing; FU Gaixia; QI Ruixia
(Affiliated Hospital of Hebei Engineering University,Handan 056001,China)
关键词:
小檗碱;  缺氧诱导因子-1α/血管内皮生长因子信号通路;  皮肤溃疡;  炎症因子;  血管生成;  创面愈合
Keywords:
Berberine;  HIF-1α/VEGF signaling pathway;  Skin ulcer;  Inflammatory factors;  Angiogenesis;  Wound healing
分类号:
R 285.5
DOI:
DOI:10.3969/j.issn.1000-7377.2024.02.006
文献标志码:
A
摘要:
目的:探究小檗碱(BR)调节缺氧诱导因子-1α(HIF-1α)/血管内皮生长因子(VEGF)信号通路对皮肤溃疡大鼠血管生成和创面愈合的影响。方法:取SD大鼠采用创面局部多次涂抹冰醋酸建立皮肤溃疡模型,随机数字法分组,包括模型组(M组)、小檗碱低剂量组(BR-L组)、小檗碱高剂量组(BR-H组)、小檗碱高剂量+空载组(BR-H+pc-DNA组)、小檗碱高剂量+HIF-1α敲低组(BR-H+pc-HIF-1α KD组),每组10只,另选10只SD大鼠作为对照组(C组)。以药物分组处理3周后,测定各组大鼠创面愈合率; HE染色检测各组大鼠皮肤溃疡创面组织病理形态; 免疫组织化学染色检测各组大鼠皮肤溃疡创面组织微血管密度(MVD)及胶原蛋白Ⅰ(Collagen Ⅰ)与纤维结合蛋白(FN)表达; 酶联免疫吸附测定(ELISA)各组大鼠血清及皮肤溃疡创面组织炎性因子IL-6、IL-8表达水平; 免疫印迹法检测各组大鼠皮肤溃疡创面组织HIF-1α/VEGF通路蛋白表达水平。结果:与C组相比,M组大鼠创面组织MVD、血清及创面组织IL-6、IL-8水平升高(均P<0.05)。与M组相比,BR-L组、BR-H组、BR-H+pc-DNA组大鼠创面愈合率、创面组织MVD、HIF-1α及VEGF蛋白表达均升高(均P<0.05),血清及创面组织IL-6、IL-8水平均降低(均P<0.05),且高剂量小檗碱作用更强。敲低HIF-1α可减弱BR对模型组大鼠各指标的作用。结论:小檗碱可通过上调HIF-1α/VEGF通路而降低炎性因子表达,抑制炎症,增强皮肤溃疡大鼠血管生成和胶原形成,促进其创面愈合。
Abstract:
Objective:To investigate the impacts of berberine(BR)on angiogenesis and wound healing in skin ulcer rats by regulating HIF-1α/VEGF signaling pathway.Methods:SD rat skin ulcer model was established by smearing glacial acetic acid multiple times on the wound surface,and SD rats were randomly divided into model group(M group),low-dose berberine group(BR-L group),high-dose berberine group(BR-H group),high-dose berberine+no load group group(BR-H+pc-DNA group),high-dose berberine+HIF-1α knockdown group(BR-H+pc-HIF-1α KD group),with 10 rats in each group,another 10 SD rats were selected as the control group(C group).After 3 weeks of treatment with drugs,the healing rate of skin ulcer wound of rats in each group was determined; HE staining was used to detect the pathological morphology of skin ulcer wound of rats in each group; the microvessel density(MVD)and the expression of collagen Ⅰand fibronectin(FN)of skin ulcer wound of rats in each group were detected by immunohistochemistry; the levels of IL-6 and IL-8 in serum and skin ulcer wound tissue of rats in each group were detected by ELISA; the expressions of HIF-1α/VEGF pathway protein in skin ulcer wound tissue of rats in each group were detected by Western blot.Results:Compared with the C group,the positive expression of MVD of wound tissue,the levels of IL-6 and IL-8 in serum and wound tissue in the M group increased(all P<0.05).Compared with the M group,the healing rate of wound,the positive expression of MVD,the expression of HIF-1α and VEGF proteins in the wound tissue in the BR-L group,the BR-H group,and the BR-H+pc-DNA group increased(all P<0.05),the levels of IL-6 and IL-8 in serum and wound tissue decreased(all P<0.05),high-dose berberine has a stronger effect.Knock down HIF-1α could weaken the effect of BR on various indicators of model group rats.Conclusion:Berberine can reduce the expression of inflammatory factors,inhibit inflammation,enhance angiogenesis and collagen formation,and promote wound healing of skin ulcer rats by up-regulating HIF-1α/VEGF signaling pathway.

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备注/Memo

备注/Memo:
基金项目:河北省医学科学研究计划项目(20210009)
更新日期/Last Update: 2024-02-05