[1]何静子,穆其琛,付东阁,等.lncRNA TMPO-AS1通过PI3K/Akt信号通路对多囊卵巢综合征大鼠性激素和卵巢功能的影响[J].陕西医学杂志,2024,(2):152-156,162.[doi:DOI:10.3969/j.issn.1000-7377.2024.02.002]
　HE Jingzi,MU Qichen,FU Dongge,et al.Effect of lncRNA TMPO-AS1 on sex hormones and ovarian function in rats with polycystic ovary syndrome through PI3K/Akt signaling pathway[J].,2024,(2):152-156,162.[doi:DOI:10.3969/j.issn.1000-7377.2024.02.002]
点击复制

lncRNA TMPO-AS1通过PI3K/Akt信号通路对多囊卵巢综合征大鼠性激素和卵巢功能的影响
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年2期
页码:
152-156,162
栏目:
基础研究
出版日期:
2024-02-05

文章信息/Info

Title:
Effect of lncRNA TMPO-AS1 on sex hormones and ovarian function in rats with polycystic ovary syndrome through PI3K/Akt signaling pathway
作者:
何静子1; 穆其琛2; 付东阁1; 王碧延3
(1.延安大学,陕西 延安 716099; 2.又石大学,韩国 全州565701; 3.延安大学附属医院,陕西 延安 716099)
Author(s):
HE Jingzi; MU Qichen; FU Dongge; WANG Biyan
(Yan'an University,Yan'an 716099,China)
关键词:
多囊卵巢综合征;  大鼠;  长链非编码RNA TMPO-AS1;  PI3K/Akt信号通路;  性激素;  卵巢功能
Keywords:
Polycystic ovary syndrome;  Rat;  lncRNA TMPO-AS1;  PI3K/Akt signaling pathway;  Sex hormones;  Ovarian function
分类号:
R 711.75
DOI:
DOI:10.3969/j.issn.1000-7377.2024.02.002
文献标志码:
A
摘要:
目的:分析长链非编码RNA(lncRNA)TMPO-AS1通过磷脂酰肌醇-3-激酶(PI3K)/蛋白激酶 B(Akt)信号通路对多囊卵巢综合征(PCOS)大鼠性激素和卵巢功能的影响。方法:随机将30只SD雌性大鼠分为模型组、si-NC组、TMPO-AS1-shRNA组,另选取健康大鼠设空白对照组,每组10只,模型组、si-NC组、TMPO-AS1-shRNA组给予60%高脂饲料,皮下注射DHEA(60 mg/kg溶于0.2 ml芝麻油中),空白对照组给予普通饲料,皮下注射等量芝麻油,于10 d开始对大鼠进行为期10 d的阴道涂片,持续注射21 d后测量大鼠体重、卵巢重量,计算卵巢指数; 并采用Western blot检测PI3K、P-PI3K、Akt、P-Akt蛋白水平; 采用荧光定量PCR(RT-qPCR)法检测TMPO-AS1、PI3K、Akt mRNA表达水平; 采用酶联免疫吸附法检测血清性激素水平; 于光学显微镜下观察卵巢形态学。结果:MPO-AS1、PI3K、Akt mRNA水平比较,模型组、si-NC组比空白对照组低(均P<0.05)。TMPO-AS1-shRNA组体重、卵巢重量、卵巢指数比空白对照组高,比模型组、si-NC组低(均P<0.05)。TMPO-AS1-shRNA组PI3K、Akt蛋白表达水平比空白对照组低,比模型组、si-NC组高(均P<0.05)。TMPO-AS1-shRNA组睾酮激素(T)、促黄体生成素(LH)水平比空白对照组高,比模型组、si-NC组低; 雌二醇(E2)、促卵泡生成激素(FSH)水平比空白对照组低,比模型组、si-NC组高(均P<0.05)。TMPO-AS1-shRNA组黄体数、成熟卵泡数、颗粒细胞厚层比空白对照组低,比模型组、si-NC组高(均P<0.05)。结论:lncRNA TMPO-AS1通过PI3K/Akt信号通路可有效降低PCOS大鼠性激素水平,并改善其卵巢功能。
Abstract:
Objective:To analyze the effects of long non-coding RNA(lncRNA)TMPO-AS1 on sex hormones and ovarian function in rats with polycystic ovary syndrome(PCOS)through phosphatidylinositol-3-kinase(PI3K)/protein kinase B(Akt)signaling pathway.Methods:Thirty female SD rats were randomly divided into model group,si-NC group and TMPO-AS1-shRNA group,and 10 healthy rats were selected as blank control group.The model group,si-NC group and TMPO-AS1-shRNA group were fed with 60% high-fat diet,and DHEA(60 mg/kg dissolved in 0.2 ml sesame oil)was injected subcutaneously.The blank control group was given normal diet,and the same amount of sesame oil was injected subcutaneously.Vaginal smears were taken for 10 days from the 10th day,and the body weight and ovarian weight of the rats were measured after 21 days of continuous injection,and Ovarian Index was calculated.The protein levels of PI3K,p-PI3K,Akt and p-Akt were detected by Western blot.The mRNA levels of TMPO-AS1,PI3K and Akt were detected by RT-qPCR.Serum sex hormone levels were detected by enzyme-linked immunosorbent assay.Ovarian morphology was observed under an optical microscope.Results:The levels of MPO-AS1,PI3K and Akt mRNA in the model group and si-NC group were significantly lower than those in the blank control group(all P<0.05).The body weight,ovarian weight and Ovarian Index of TMPO-AS1-shRNA group were higher than those of blank control group,and lower than those of model group and si-NC group(all P<0.05).The protein expression levels of PI3K,Akt in TMPO-AS1-shRNA group were lower than those in blank control group,and higher than those in model group and si-NC group(all P<0.05).The levels of testosterone(T)and luteinizing hormone(LH)in TMPO-AS1-shRNA group were higher than those in blank control group,and lower than those in model group and si-NC group(all P<0.05).The levels of estradiol(E2)and follicle stimulating hormone(FSH)were lower than those in the blank control group,and higher than those in the model group and si-NC group(all P<0.05).The number of corpus luteum,the number of mature follicles and the thickness of granulosa cells in TMPO-AS1-shRNA group were lower than those in blank control group,and higher than those in model group and si-NC group(all P<0.05).Conclusion:lncRNA TMPO-AS1 can effectively reduce sex hormone levels and improve ovarian function in PCOS rats through PI3K/Akt signaling pathway.

参考文献/References:

[1] 钟社兰,陈星.多囊卵巢综合征与失眠症共病患者临床特征及相关因素分析[J].陕西医学杂志,2023,52(4):437-440.
[2] 李怡欣,李盼盼,王永宏.王永宏教授治疗多囊卵巢综合征所致排卵障碍性不孕经验[J].陕西中医,2022,43(5):636-639,643.
[3] 陈然,刘雁峰,王乾平,等.多囊卵巢综合征排卵障碍性不孕的临床治疗经验[J].中日友好医院学报,2023,37(1):44-46.
[4] 刘琳,王静怡,王欣,等.高通量测序应用于PCOS患者卵泡液外泌体lncRNA表达谱分析[J].生殖医学杂志,2020,29(5):620-627.
[5] 邓俊亮,华美香,陈键腾.lncRNA TMPO-AS1通过调节miR-204-3p影响肺癌细胞的增殖、凋亡和侵袭[J].中国免疫学杂志,2021,37(3):319-324,329.
[6] 高玥,丁锦丽,杨菁.PI3K/Akt信号通路在多囊卵巢综合征发病中的作用研究进展[J].医学综述,2022,28(3):563-568.
[7] 徐云霞,徐珺萍,卞景新.基于PI3K/Akt通路研究孕育丹糖浆防治大鼠多囊卵巢综合征不孕症的机制[J].中成药,2022,44(7):2285-2290.
[8] 张艺,张继,叶彩云,等.芍药苷对多囊卵巢综合征大鼠卵巢颗粒细胞增殖和凋亡的影响机制[J].实用药物与临床,2023,26(7):581-586.
[9] 李婷,李雪倩,尹东升.多囊卵巢综合征患者妊娠期体内维生素D水平与血脂代谢以及妊娠结局的关系[J].陕西医学杂志,2022,51(1):55-58.
[10] 李永乐,张丽,申小静,等.多囊卵巢综合征患者外周血长链非编码NTF3-5表达及与胰岛素抵抗关系[J].中国计划生育学杂志,2022,30(6):1278-1281.
[11] 吴镔莎,余宁,卢佳敏,等.多囊卵巢综合征患者血清特异性lncRNA表达谱研究[J].浙江医学,2020,42(8):798-801,882.
[12] 祁彦萍,王英,杨建敏.长链非编码RNA GAS5、微小RNA-21在多囊卵巢综合征中的表达及生物学意义[J].安徽医药,2021,25(2):304-308.
[13] 余晓燕,杨惠林,王烈宏.lncRNA ZFAS1调节miR-136-5p/IGF1R轴对多囊卵巢综合征小鼠胰岛素抵抗的影响[J].中国优生与遗传杂志,2022,30(8):1365-1370.
[14] 周斌锋,彭兵锋,王志强,等.lncRNA TMPO-AS1调控miR-143-3p的表达影响肺癌细胞增殖、侵袭、迁移和凋亡[J].中国老年学杂志,2021,41(15):3320-3328.
[15] ZHAO H,DING F,ZHENG G.lncRNA TMPO-AS1 promotes LCN2 transcriptional activity and exerts oncogenic functions in ovarian cancer[J].FASEB J,2020,34(9):11382-11394.
[16] 郑小妹,林叶飞,张韶琼,等.IGF1调控PI3K/Akt信号通路对卵巢癌细胞增殖和侵袭的影响[J].中国计划生育和妇产科,2023,15(4):32-37.
[17] LIU M,QIU Y,XUE Z,et al.Small extracellular vesicles derived from embryonic stem cells restore ovarian function of premature ovarian failure through PI3K/Akt signaling pathway[J].Stem Cell Res Ther,2020,11(1):3.
[18] 代东宇,朱雯博,王泽,等.Med19通过PTEN-PI3K/Akt信号通路影响卵巢癌细胞的生长[J].哈尔滨医科大学学报,2021,55(3):244-247.
[19] 尹丽红,焦琳,陈裕,等.多囊卵巢综合征大鼠子宫内膜中PI3K/Akt通路相关蛋白表达异常与胰岛素抵抗的关系[J].中国实验动物学报,2022,30(6):777-783.
[20] 朱美文,岳德永,曹作增,等.加味芎归二陈汤联合西药治疗痰湿型多囊卵巢综合征疗效及对患者糖脂代谢和血清性激素水平的影响[J].陕西中医,2022,43(4):442-445.
[21] 陈娇,张妍,钟媛媛.microRNA-141通过PI3K/Akt/mTOR信号通路促进PCOS卵巢颗粒细胞增殖的机制研究[J].天津医药,2021,49(7):673-678.
[22] XIE F,ZHANG J,ZHAI M,et al.Melatonin ameliorates ovarian dysfunction by regulating autophagy in PCOS via the PI3K/Akt pathway[J].Reproduction,2021,162(1):73-82.
[23] 刘菏婧,刘雪峰,张楚楚,等.补肾化痰活血方调控PI3K/Akt通路改善多囊卵巢综合征-胰岛素抵抗大鼠的作用机制研究[J].中国中医基础医学杂志,2023,29(5):753-760.
[24] HOU F,LI J,PENG J,et al.lncRNA TMPO-AS1 suppresses the maturation of miR-335-5p to participate in polycystic ovary syndrome[J].J Ovarian Res,2021,14(1):99.
[25] 王毅,王海娇,祁麟,等.桃核承气汤对多囊卵巢综合征大鼠卵巢组织PI3K/Akt/mTOR信号通路的影响[J].天津医药,2023,51(6):596-601.

相似文献/References:

[1]王钰,李小燕,宋民喜,等.不同表型多囊卵巢综合征患者甲状腺功能改变及临床意义[J].陕西医学杂志,2016,(10):1303.
[2]李丽丽,师萍萍△.血黄体生成素检测、经阴道超声卵泡监测联合不同促排卵方案 对多囊卵巢综合征患者妊娠结局的影响*[J].陕西医学杂志,2020,49(1):64.
　LI Lili,SHI Pingping..Effects of blood LH test,transvaginal ultrasound follicle monitoring combined with different ovulation induction regimens on pregnancy outcome in patients with polycystic ovary syndrome[J].,2020,49(2):64.
[3]王玉珏,辛宁宁△.mGluR6对大鼠胚胎神经干细胞生物学功能的影响*[J].陕西医学杂志,2020,49(3):279.
[4]蔡碧莲,文亦磊,罗伟生△.四氯化碳溶液构建肝纤维化大鼠模型研究进展*[J].陕西医学杂志,2020,49(5):639.
[5]刘文强,刘学政,左中夫△.RU486通过Notch1/Hes-1信号通路对糖尿病大鼠视网膜神经节细胞的保护作用及机制研究*[J].陕西医学杂志,2020,49(6):656.[doi:DOI:10.3969/j.issn.10007377.2020.06.004]
　LIU Wenqiang,LIU Xuezheng,ZUO Zhongfu..Protective effect of RU486 on retinal ganglion cells in diabetic rats via Notch1/Hes-1 signaling pathway[J].,2020,49(2):656.[doi:DOI:10.3969/j.issn.10007377.2020.06.004]
[6]蔡碧莲,文亦磊△,罗伟生△.大鼠肝纤维化实验动物模型的构建*[J].陕西医学杂志,2020,49(6):659.[doi:DOI:10.3969/j.issn.10007377.2020.06.005]
[7]朱美霖,白宏英.调控Sonic Hedgehog信号通路对急性脑缺血大鼠血管再生影响的实验研究*[J].陕西医学杂志,2020,49(7):774.[doi:DOI:10.3969/j.issn.10007377.2020.07.002]
　ZHU Meilin,BAI Hongying..Effect of regulating SHH signaling pathway on angiogenesis in rats with acute cerebral ischemia[J].,2020,49(2):774.[doi:DOI:10.3969/j.issn.10007377.2020.07.002]
[8]艾丁丁,罗伟生△,蒋云霞.动物肝纤维化模型建立研究进展*[J].陕西医学杂志,2020,49(7):907.[doi:DOI:10.3969/j.issn.10007377.2020.07.038]
　AI Dingding,LUO Weisheng,JIANG Yunxia..Progress in the establisment of animal liver fibrosis model[J].,2020,49(2):907.[doi:DOI:10.3969/j.issn.10007377.2020.07.038]
[9]朱美霖,白宏英.Purmorphamine对脑缺血再灌注大鼠细胞凋亡影响的实验研究[J].陕西医学杂志,2021,50(1):7.[doi:DOI:10.3969/j.issn.1000-7377.2021.01.002]
　ZHU Meilin,BAI Hongying.Effect of Purmorphamine on apoptosis in rats with cerebral ischemia-reperfusion[J].,2021,50(2):7.[doi:DOI:10.3969/j.issn.1000-7377.2021.01.002]
[10]肖小娟,魏 星,赵莎彤,等.3-甲基腺嘌呤腹腔注射干预大鼠胃窦组织自噬最佳药物剂量与用药时间探讨[J].陕西医学杂志,2021,50(2):131.[doi:DOI:10.3969/j.issn.1000-7377.2021.02.001]
　XIAO Xiaojuan,WEI Xing,ZHAO Shatong,et al.Optimal dose and time of intraperitoneal injection of 3-MA for intervention of gastric antrum autophagy in rats[J].,2021,50(2):131.[doi:DOI:10.3969/j.issn.1000-7377.2021.02.001]
[11]杨加宁,张立然.沉默信息调节因子1/叉头框蛋白O1信号通路调控铁死亡对多囊卵巢综合征大鼠卵巢颗粒细胞的影响实验研究[J].陕西医学杂志,2024,(8):1035.[doi:DOI:10.3969/j.issn.1000-7377.2024.08.006]
　YANG Jianing,ZHANG Liran.Effect of silent information regulator 1/forkhead box protein O1 signaling pathway on ovarian granulosa cells in rats with polycystic ovary syndrome by regulating ferroptosis[J].,2024,(2):1035.[doi:DOI:10.3969/j.issn.1000-7377.2024.08.006]

备注/Memo

备注/Memo:
基金项目:陕西省教育厅专项科研计划项目(23JK0733); 陕西省体育局常规科研项目(2023020); 延安大学青年博士科研项目(YDBK2022-20,YDBK2022-115)
更新日期/Last Update: 2024-02-05