[1]刘毅力,郭 冲,尹 恒,等.环状RNA circ-TAGLN在卵巢癌细胞中的表达及对卵巢癌细胞增殖和侵袭的影响[J].陕西医学杂志,2024,(2):147-151.[doi:DOI:10.3969/j.issn.1000-7377.2024.02.001]
　LIU Yili,GUO Chong,YIN Heng,et al.Expression of circular RNA circ-TAGLN in ovarian cancer and its effect on ovarian cancer cell proiferation and invasion[J].,2024,(2):147-151.[doi:DOI:10.3969/j.issn.1000-7377.2024.02.001]
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环状RNA circ-TAGLN在卵巢癌细胞中的表达及对卵巢癌细胞增殖和侵袭的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年2期
页码:
147-151
栏目:
基础研究
出版日期:
2024-02-05

文章信息/Info

Title:
Expression of circular RNA circ-TAGLN in ovarian cancer and its effect on ovarian cancer cell proiferation and invasion
作者:
刘毅力1; 郭 冲1; 尹 恒1; 刘 洋2
(1.湖北医药学院附属太和医院妇产中心,湖北 十堰 442000; 2.昆明医科大学第一附属医院肿瘤科,云南 昆明 650032)
Author(s):
LIU Yili; GUO Chong; YIN Heng; LIU Yang
(Obstetrics and Gynecology Center,Taihe Hospital Affiliated to Hubei Medical College,Shiyan 442000,China)
关键词:
卵巢肿瘤;  circ-TAGLN;  miR-425-5p;  细胞增殖;  细胞侵袭;  分子机制
Keywords:
Ovarian tumor;  circ-TAGLN;  miR-425-5p;  Cell proliferation;  Cell invasion;  Molecular mechanism
分类号:
R 737.31
DOI:
DOI:10.3969/j.issn.1000-7377.2024.02.001
文献标志码:
A
摘要:
目的:探讨环状RNA(circRNA)circ-TAGLN在卵巢癌细胞中的表达情况和过表达circ-TAGLN对卵巢癌细胞增殖和侵袭的影响及分子机制。方法:采用实时荧光定量聚合酶链反应(RT-qPCR)技术分析circ-TAGLN在卵巢癌细胞系SK-OV-3、OC3、Caov-3、A2780、HO-8910和正常卵巢上皮细胞IOSE80中的表达差异。分别转染pcDNA3.1对照质粒(NC组)和pcDNA3.1-circ-TAGLN质粒(circ-TAGLN组)至SK-OV-3细胞。利用平板克隆实验和Transwell实验检测卵巢癌SK-OV-3细胞的增殖和侵袭能力。双荧光素酶报告实验验证circ-TAGLN与miR-425-5p的靶向关系。利用RT-qPCR技术分析过表达circ-TAGLN对SK-OV-3细胞中miR-425-5p表达的影响。Western blot检测过表达circ-TAGLN对SK-OV-3细胞中PI3K/AKT信号通路蛋白p-PI3K、p-AKT、p-mTOR、VEGF、eNOS表达的影响。结果:与IOSE80细胞相比,circ-TAGLN在卵巢癌细胞系SK-OV-3、OC3、Caov-3、A2780、HO-8910中低表达(均P<0.05)。NC组与circ-TAGLN组中circ-TAGLN相对表达分别为(1.01±0.34)和(7.56±1.07),circ-TAGLN组SK-OV-3细胞中circ-TAGLN表达高于NC组(P<0.01)。在SK-OV-3细胞中,过表达circ-TAGLN后细胞增殖能力和侵袭能力均下降(均P<0.01)。circ-TAGLN靶向结合miR-425-5p(P<0.01)。NC组与circ-TAGLN组SK-OV-3细胞中miR-425-5p表达分别为(5.57±0.91)和(1.01±0.17),与NC组相比,过表达circ-TAGLN后SK-OV-3细胞中miR-425-5p表达被抑制(P<0.01)。在SK-OV-3细胞中,过表达circ-TAGLN后细胞中PI3K/AKT信号通路蛋白p-PI3K、p-AKT、p-mTOR、VEGF、eNOS表达下降(均P<0.01)。结论:circ-TAGLN在卵巢癌细胞中低表达,并能通过靶向降低miR-425-5p表达抑制卵巢癌细胞的增殖和侵袭,circ-TAGLN可作为卵巢癌潜在的分子治疗靶点。
Abstract:
Objective:To explore the expression of circular RNA(circRNA)circ-TAGLN in ovarian cancer and the effects and molecular mechanisms of overexpression of circ-TAGLN on the proliferation and invasion of ovarian cancer cells.Methods:RT-qPCR was used to analyze the expression differences of circ-TAGLN in ovarian cancer cell lines SK-OV-3,OC3,Caov-3,A2780,HO-8910 and normal ovarian epithelial cells IOSE80.The pcDNA3.1 control plasmid(NC group)and pcDNA3.1-circ-TAGLN plasmid(circ-TAGLN group)were transfected into SK-OV-3 cells respectively.The proliferation and invasion abilities of ovarian cancer SK-OV-3 cells were detected using plate cloning assay and Transwell assay.Dual-luciferase reporter experiment verified the targeting relationship between circ-TAGLN and miR-425-5p.The effect of overexpression of circ-TAGLN on the expression of miR-425-5p in SK-OV-3 cells was analyzed using RT-qPCR.Western blot was used to detect the effect of overexpression of circ-TAGLN on the expression of PI3K/AKT signaling pathway proteins p-PI3K,p-AKT,p-mTOR,VEGF,and eNOS in SK-OV-3 cells.Results:Compared with IOSE80 cells,circ-TAGLN was lowerly expressed in ovarian cancer cell lines SK-OV-3,OC3,Caov-3,A2780,and HO-8910(all P<0.05).The relative expressions of circ-TAGLN in the NC group and circ-TAGLN group were(1.01±0.34)and(7.56±1.07)respectively,and the expression of circ-TAGLN in SK-OV-3 cells in the circ-TAGLN group was higher than that in the NC group(P<0.01).In SK-OV-3 cells,overexpression of circ-TAGLN reduced cell proliferation and invasion abilities(both P<0.01).circ-TAGLN targeted and binded to miR-425-5p.The expression of miR-425-5p in SK-OV-3 cells in the NC group and circ-TAGLN group were(5.57±0.91)and(1.01±0.17)respectively.Compared with the NC group,the expression of miR-425-5p in SK-OV-3 was inhibited(P<0.01).In SK-OV-3 cells,overexpression of circ-TAGLN reduced the expression of PI3K/AKT signaling pathway proteins p-PI3K,p-AKT,p-mTOR,VEGF,and eNOS in the cells.Conclusion:circ-TAGLN is lowly expressed in ovarian cancer and can inhibit the proliferation and invasion of ovarian cancer cells by targeting the reduction of miR-425-5p expression.circ-TAGLN can be used as a potential molecular therapeutic target for ovarian cancer.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81860515)
更新日期/Last Update: 2024-02-05