[1]王松海,凡 瑾,陈 捷.红景天苷对Lewis 肺癌小鼠恶性胸腔积液的抑制作用及其对免疫功能的影响[J].陕西医学杂志,2024,(1):37-41.[doi:DOI:10.3969/j.issn.1000-7377.2024.01.008]
　WANG Songhai,FAN Jin,CHEN Jie.Inhibitory effect of SALidroside on malignant pleural effusion and its effect on immune function in mice with Lewis lung cancer[J].,2024,(1):37-41.[doi:DOI:10.3969/j.issn.1000-7377.2024.01.008]
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红景天苷对Lewis 肺癌小鼠恶性胸腔积液的抑制作用及其对免疫功能的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年1期
页码:
37-41
栏目:
基础研究
出版日期:
2024-01-31

文章信息/Info

Title:
Inhibitory effect of SALidroside on malignant pleural effusion and its effect on immune function in mice with Lewis lung cancer
作者:
王松海1; 凡 瑾2; 陈 捷1
(1.陕西省中医医院肿瘤科,陕西 西安 710003; 2.陕西中医药大学,陕西 咸阳 712046)
Author(s):
WANG Songhai; FAN Jin; CHEN Jie
(Department of Oncology,Shaanxi Provincial Hospital of Traditional Chinese Medicine,Xi'an 710002,China)
关键词:
红景天苷;  胸腔积液;  肺癌;  顺铂;  血管内皮生长因子;  肿瘤坏死因子-α
Keywords:
Salidroside;  Pleural effusion;  Lung cancer;  Cisplatin;  VEGF;  TNF-α
分类号:
R 734.2
DOI:
DOI:10.3969/j.issn.1000-7377.2024.01.008
文献标志码:
A
摘要:
目的:探究红景天苷(SAL)对肺癌小鼠恶性胸腔积液生成的抑制作用及对其免疫功能的影响。方法:通过胸腔注射Lewis肺癌细胞(LLC)建立C57BL/6小鼠恶性胸腔积液(MPE)模型; 建模后随机分为正常组、模型组、SAL组、顺铂组(DDP组)、红景天苷+顺铂联合组(SAL+DDP组); 实验结束后,收集各组小鼠胸水并测量胸水体积、生存周期。酶联免疫吸附法(ELISA)检测MPE小鼠血清中肿瘤坏死因子-α(TNF-α)、低氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)水平; 流式细胞仪定量检测T细胞亚群CD3+、CD4+、CD8+、CD4+/CD8+及自然杀伤(NK)细胞百分比。结果:MPE模型小鼠经治疗后,与模型组比较,DDP组、联合组小鼠生存周期显著延长(均P<0.01); SAL组、DDP组和联合组MPE体积均显著减少(均P<0.01); SAL组、DDP组和联合组外周血中TNF-α水平显著升高,HIF-1α、VEGF水平显著降低(均P<0.05); SAL组、DDP组和联合组CD3+均显著升高(均P<0.05); SAL组、联合组CD4+显著升高(均P<0.05),DDP组升高无统计学意义(P>0.05); SAL组、联合组CD8+、CD4+/CD8+、NK均显著升高(均P<0.05),DDP组降低无统计学意义(P>0.05)。结论:SAL联合顺铂胸腔灌注抑制肺癌小鼠MPE生成效果显著,可能与HIF-1α-VEGF分子通路抑制以及免疫功能激活有关。
Abstract:
Objective:To investigate the inhibitory effect of salidroside(SAL)on the formation of malignant pleural effusion and its effect on immune function in mice with lung cancer.Methods:The malignant pleural effusion(MPE)model of C57BL/6 mice was established by thoracic injection of Lewis lung cancer cells(LLC).After modeling,they were randomly divided into normal group,model group,SAL group,cisplatin group(DDP group)and salidroside + cisplatin combination group(SAL+DDP group).After the experiment,the pleural fluid of each group was collected and its volume and life cycle were measured.The levels of tumor necrosis factor(TNF-α),hypoxia inducible factor 1alpha(HIF-1α)and vascular endothelial growth factor(VEGF)in serum of MPE mice were detected by ELISA.T cell subsets CD3+,CD4+,CD8+,CD4+/CD8+ and the percentage of NK cells were quantitatively detected by flow cytometry.Results:After treatment,compared with the model group,the survival period of the MPE model mice in the DDP group and the combination group was significantly prolonged(all P<0.01); the MPE volumes of SAL group,DDP group and combination group were significantly reduced(all P<0.01); the levels of TNF-α in peripheral blood of SAL group,DDP group and combination group were significantly increased,and the levels of HIF-1α and VEGF were significantly decreased(all P<0.05); CD3+ in SAL group,DDP group and combination group were significantly increased(all P<0.05); CD4+ was significantly increased in SAL group and combination group(all P<0.05),but not in DDP group(P>0.05); CD8+,CD4+/CD8+ and NK were significantly increased in SAL group and combination group(all P<0.05),but the decrease in DDP group was not statistically significant(P>0.05).Conclusion:SAL combined with cisplatin perfusion can significantly inhibit MPE production in lung cancer mice,which may be related to inhibition of HIF-1α-VEGF molecular pathway and activation of immune function.

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备注/Memo

备注/Memo:
基金项目:陕西省自然科学基础研究项目(2020JM-670)
更新日期/Last Update: 2024-01-05