[1]张亮亮,赵程锦,周煜虎,等.积雪草酸对大鼠绝经后骨质疏松症的治疗作用及机制研究[J].陕西医学杂志,2023,52(12):1624-1630.[doi:DOI:10.3969/j.issn.1000-7377.2023.12.002]
　ZHANG Liangliang,ZHAO Chengjin,ZHOU Yuhu,et al.Therapeutic effect and mechanism of asiatic acid on postmenopausal osteoporosis in rats[J].,2023,52(12):1624-1630.[doi:DOI:10.3969/j.issn.1000-7377.2023.12.002]
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积雪草酸对大鼠绝经后骨质疏松症的治疗作用及机制研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年12期
页码:
1624-1630
栏目:
基础研究
出版日期:
2023-12-05

文章信息/Info

Title:
Therapeutic effect and mechanism of asiatic acid on postmenopausal osteoporosis in rats
作者:
张亮亮; 赵程锦; 周煜虎; 曹 博; 段明明; 冯阳阳
(延安大学附属医院创伤骨科,陕西 延安 716000)
Author(s):
ZHANG Liangliang; ZHAO Chengjin; ZHOU Yuhu; CAO Bo; DUAN Mingming; FENG Yangyang
(Department of Traumatic Orthopedics,Affiliated Hospital of Yan'an University,Yan'an 716000,China)
关键词:
骨质疏松症;  积雪草酸;  Hedgehog信号通路;  骨代谢;  微结构;  大鼠
Keywords:
Osteoporosis;  Asiatic acid;  Hedgehog signaling pathway;  Bone metabolism;  Microstructure;  Rat
分类号:
R 580
DOI:
DOI:10.3969/j.issn.1000-7377.2023.12.002
文献标志码:
A
摘要:
目的:探讨积雪草酸对大鼠绝经后骨质疏松症(OP)的治疗作用及相关机制。方法:采用一次性手术摘除双侧卵巢方式构建绝经后OP大鼠模型,分为模型组(9只)、雌二醇组(10只)、积雪草酸低剂量组(10只)、积雪草酸中剂量组(10只)和积雪草酸高剂量组(10只)。另取9只大鼠作为假手术组。观察骨组织病理改变、骨密度及微结构,以及血清骨代谢指标、骨代谢相关基因及蛋白表达。结果:雌二醇组和积雪草酸低、中、高剂量组均可减轻骨组织病理改变,增加骨密度,改善骨微结构。与假手术组比较,模型组大鼠股骨骨密度(BMD)、骨体积分数(BV/TV)、骨小梁数目(Tb.N)、骨小梁厚度(Tb.Th)下降,结构模式指数(SMI)和骨小梁分离度(Tb.Sp)上升,血清骨碱性磷酸酶(BALP)水平下降,Ⅰ型胶原交联氨基端肽(NTX-Ⅰ)和抗酒石酸酸性磷酸酶(TRAP)水平上升,骨组织音猬因子(SHH)、细胞表面跨膜蛋白Patched1(Ptc1)、7次跨膜蛋白(Smo)、Gli家族锌指蛋白-1(Gli1)、矮小相关转录因子2(Runx2)、骨保护素(OPG)、成骨细胞特异性转录因子(Osx)mRNA和蛋白表达下降,细胞核因子-κB受体活化因子(RANKL)mRNA和蛋白表达上升(均P<0.05)。与模型组比较,雌二醇组和积雪草酸低、中、高剂量组大鼠股骨BMD、BV/TV、Tb.N、Tb.Th上升,SMI和Tb.Sp下降,血清BALP水平上升,NTX-Ⅰ和TRAP水平下降,骨组织SHH、Ptc1、Smo、Gli1、Runx2、OPG、Osx mRNA和蛋白表达上升,RANKL mRNA和蛋白表达下降(均P<0.05)。雌二醇组和积雪草酸低剂量组上述指标比较差异无统计学意义(均P>0.05)。积雪草酸低、中、高剂量组上述指标呈剂量依赖性(均P<0.05)。结论:积雪草酸可治疗绝经后OP,可能与激活Hedgehog信号通路相关。
Abstract:
Objective:To investigate the therapeutic effect of asiatic acid on postmenopausal osteoporosis(OP)rats and its related mechanism.Methods:Postmenopausal OP rats model was constructed by using disposable surgical excision bilateral ovaries,and divided into model group(9 rats),estradiol group(10 rats),asiatic acid low-dose group(10 rats),asiatic acid medium-dose group(10 rats)asiatic acid high-dose group(10 rats),another 9 rats were enrolled sham-operation group.The pathological changes,bone density and microstructure of bone tissue,serum bone metabolism indexes,and the expression of bone metabolism-related genes and proteins were observed.Results:The estradiol group and the asiatic acid low-,medium-,and high-dose groups could reduce the pathological changes of bone tissue,increase bone mineral density,and improve bone microstructure.Compared with the sham-operation group,in the model group,the femoral BMD,BV/TV,Tb.N,Tb.Th decreased,SMI and Tb.Sp increased,and serum BALP level decreased,NTX-Ⅰ and TRAP increased,and the mRNA and protein expressions of SHH,Ptc1,Smo,Gli1,Runx2,OPG,Osx in bone tissue decreased,RANKL mRNA and protein expression increased(all P<0.05).Compared with the model group,in the estradiol group and the asiatic acid low-,medium-,and high-dose groups,BMD,BV/TV,Tb.N,Tb.Th increased,SMI and Tb.Sp decreased,serum BALP level increased,and NTX-Ⅰ and TRAP levels decreased,and the mRNA and protein expressions of SHH,Ptc1,Smo,Gli1,Runx2,OPG,and Osx in bone tissue increased,while RANKL mRNA and protein expression decreased(all P<0.05).There was no significant difference in the above indicators between the estradiol group and the asiatic acid low-dose group(all P>0.05).The above indicators in the asiatic acid low-,medium- and high-dose groups showed a dose-dependent manner(all P<0.05).Conclusion:Asiatic acid can treat osteoporosis,which may be related to activating Hedgehog signaling pathway.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(82160431)
更新日期/Last Update: 2023-12-05