[1]吴文婷,李 敏,蒲文娟.白藜芦醇对慢性阻塞性肺疾病大鼠p38丝裂原活化蛋白激酶/核因子-κB信号通路的影响[J].陕西医学杂志,2023,52(11):1489-1492,1534.[doi:DOI:10.3969/j.issn.1000-7377.2023.11.008]
　WU Wenting,LI Min,PU Wenjuan.Effect of resveratrol on p38 mitogen-activated protein kinase/NF-κB signaling pathway in rats with chronic obstructive pulmonary disease[J].,2023,52(11):1489-1492,1534.[doi:DOI:10.3969/j.issn.1000-7377.2023.11.008]
点击复制

白藜芦醇对慢性阻塞性肺疾病大鼠p38丝裂原活化蛋白激酶/核因子-κB信号通路的影响
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年11期
页码:
1489-1492,1534
栏目:
基础研究
出版日期:
2023-11-05

文章信息/Info

Title:
Effect of resveratrol on p38 mitogen-activated protein kinase/NF-κB signaling pathway in rats with chronic obstructive pulmonary disease
作者:
吴文婷; 李 敏; 蒲文娟
(空军军医大学唐都医院呼吸与危重症医学科,陕西 西安710038)
Author(s):
WU Wenting; LI Min; PU Wenjuan
(Department of Respiratory and Critical Care Medicine,Tangdu Hospital of Air Force Medical University,Xi'an 710038,China)
关键词:
慢性阻塞性肺疾病;  白藜芦醇;  肺损伤;  炎症因子;  p38丝裂原活化蛋白激酶/核因子-κB信号通路;  大鼠
Keywords:
Chronic obstructive pulmonary disease;  Resveratrol;  Lung damage;  Inflammation factor;  p38 MAPK/NF-κB signaling pathway;  Rats
分类号:
R 562.21
DOI:
DOI:10.3969/j.issn.1000-7377.2023.11.008
文献标志码:
A
摘要:
目的:探讨白藜芦醇对慢性阻塞性肺疾病(COPD)大鼠p38丝裂原活化蛋白激酶(MAPK)/核因子-κB(NF-κB)信号通路的影响。方法:将50只SD大鼠随机分为正常组,模型组,白藜芦醇低剂量组、白藜芦醇中剂量组、白藜芦醇高剂量组,每组10只。模型组及白藜芦醇组采用烟熏联合脂多糖气道内注射法复制COPD大鼠模型,模型构建成功后,白藜芦醇低、中、高剂量组大鼠分别灌胃给予5、15、45 mg/(kg·d)白藜芦醇,正常组及模型组给予等量0.9%氯化钠溶液,连续28 d。HE染色观察肺组织病理形态,分光光度法检测丙二醛(MDA)及超氧化物歧化酶(SOD)水平,酶联免疫吸附(ELISA)法检测肺组织中肿瘤坏死因子-α(TNF-α),白介素-8(IL-8),基质金属蛋白酶-9(MMP-9)及基质金属蛋白酶抑制剂-1(TIMP-1)含量,Western blot检测p38MAPK/NF-κB信号通路相关蛋白表达情况。结果:与模型组比较,白藜芦醇低、中、高剂量组肺泡结构较为完整,有少量炎性细胞浸润,TNF-α、IL-8、MDA、MMP-9 含量降低、p38 MAPK、核因子κB抑制因子α(IκB α)及NF-κB p65 表达下调,SOD活性及TIMP-1含量升高。结论:白藜芦醇能够缓解COPD大鼠肺损伤,可能与抑制p38 MAPK/NF-κB信号通路有关。
Abstract:
Objective:To explore the effect of resveratrol on p38 mitogen activated protein kinase(MAPK)/nuclear factor-κB(NF-κB)signaling pathway in rats with chronic obstructive pulmonary disease(COPD).Methods:Fifty SD rats were randomly divide into normal group,model group,resveratrol low-,medium-,and high-dose groups,with 10 rats in each group.Model and resveratrol group was established by smoking combined with lipopolysaccharide airway injection method.After successful model construction,resveratrol low-,medium-,and high-dose groups were given 5,15,45 mg/(kg·d)resveratrol by gavage,respectively.The normal group and model group were given equal amounts of 0.9% sodium chloride solution,for 28 consecutive days.The pathological morphology of lung tissue was detected by HE staining.The levels of malondialdehyde(MDA)and superoxide dismutase(SOD)were detected by spectrophotometry.The levels of tumor necrosis factor in lung tissue-α(TNF-α),interleukin-8(IL-8),matrix metalloproteinase-9(MMP-9)andtissue inhibitor of metalloproteinases-1(TIMP-1)were detected by ELISA.The expressions of p38MAPK/NF-κB signal pathway related protein were detected by Western blot.Results:Compared with the model group,in the resveratrol low-,medium-,and high-dose groups,the alveolar structure was relatively complete,with a small amount of inflammatory factor infiltration,the levels of TNF-α,IL-8,MDA,MMP-9,the expressions of p38 MAPK,inhibitor of NF-κB(IκB α)and NF-κB p65 were decreased,the activity of SOD and level of TIMP-1 were increased.Conclusion:Resveratrol can alleviate lung damage in COPD rats,which may be related to the inhibition of p38MAPK/NF-κB signaling pathway.

参考文献/References:

[1] Christenson SA,Smith BM,Bafadhel M,et al.Chronic obstructive pulmonary disease[J].Lancet,2022,399(10342):2227-2242.
[2] Ruvuna L,Sood A.Epidemiology of chronic obstructive pulmonary disease[J].Clin Chest Med,2020,41(3):315-327.
[3] 窦 飞,王国清,宋东虎,等.CT评估慢性阻塞性肺疾病肺功能临床研究进展[J].陕西医学杂志,2023,52(7):942-945.
[4] Wang C,Xu J,Yang L,et al.Prevalence and risk factors of chronic obstructive pulmonary disease in China:A national cross-sectional study[J].Lancet,2018,391(10131):1706-1717.
[5] Arezina R,Chen T,Wang D.Conventional,complementary and alternative medicines:Mechanistic insights into therapeutic landscape of chronic obstructive pulmonary disease[J].Int J Chron Obstruct Pulmon Dis,2023,18:447-457.
[6] Ma BN,Li XJ.Resveratrol extracted from Chinese herbal medicines:A novel therapeutic strategy for lung diseases[J].Chin Herb Med,2020,12(4):349-358.
[7] 朱汉平,王 鹏,李振球,等.清肺理痰方对COPD模型大鼠呼吸功能的影响及其机制[J].中国老年学杂志,2021,41(11):2381-2385.
[8] 许海江,欧阳松云,陈兰兰,等.赤芍总苷对慢性阻塞性肺疾病模型大鼠的干预作用[J].中国新药与临床杂志,2022,41(9):561-565.
[9] 曾德优,张六一,肖 创,等.慢性阻塞性肺疾病动物模型研究进展[J].陕西医学杂志,2022,51(4):507-510.
[10] Hikichi M,Mizumura K,Maruoka S,et al.Pathogenesis of chronic obstructive pulmonary disease(COPD)induced by cigarette smoke[J].J Thorac Dis,2019,11(17):2129-2140.
[11] Adeloye D,Song P,Zhu Y,et al.Global,regional,and national prevalence of,and risk factors for,chronic obstructive pulmonary disease(COPD)in 2019:A systematic review and modelling analysis[J].Lancet Respir Med,2022,10(5):447-458.
[12] 肖心儒,施宇佳,张 倩.慢性阻塞性肺疾病生物学标志物的研究进展[J].南京医科大学学报:自然科学版,2023,43(7):1011-1016.
[13] 蔡东林,孙钟和,赵益庆.慢性阻塞性肺疾病稳定期BODE指数、IL-8、TNF-α水平与肺肾气虚证相关性研究[J].陕西中医,2020,41(11):1573-1575.
[14] Barnes PJ.Oxidative stress in chronic obstructive pulmonary disease[J].Antioxidants(Basel),2022,11(5):965.
[15] Pelaia C,Vatrella A,Gallelli L,et al.Role of p38 mitogen-activated protein kinase in asthma and COPD:Pathogenic aspects and potential targeted therapies[J].Drug Des Devel Ther,2021,15:1275-1284.
[16] Li Q,Cui K,Wu M,et al.Polyunsaturated fatty acids influence LPS-induced inflammation of fish macrophages through differential modulation of pathogen recognition and p38 MAPK/NF-kappaB signaling[J].Front Immunol,2020,11:559332.
[17] Pelaia C,Vatrella A,Sciacqua A,et al.Role of p38-mitogen-activated protein kinase in COPD:Pathobiological implications and therapeutic perspectives[J].Expert Rev Respir Med,2020,14(5):485-491.
[18] 孙境孺,李竹英.NF-κB信号通路在肺部疾病中的作用研究进展及中医药干预现状[J].陕西中医,2022,43(7):972-974.
[19] Hankittichai P,Thaklaewphan P,Wikan N,et al.Resveratrol enhances cytotoxic effects of cisplatin by inducing cell cycle arrest and apoptosis in ovarian adenocarcinoma SKOV-3 cells through activating the p38 MAPK and suppressing AKT[J].Pharmaceuticals(Basel),2023,16(5):755.
[20] Cao X,Tian S,Fu M,et al.Resveratrol protects human bronchial epithelial cells against nickel-induced toxicity via suppressing p38 MAPK,NF-kappaB signaling,and NLRP3 inflammasome activation[J].Environ Toxicol,2020,35(5):609-618.

相似文献/References:

[1]马明铭,李 芳,杨 慧,等.噻托溴胺粉雾剂联合非药物康复方案对慢性阻塞性肺疾病患者BODE指数的影响[J].陕西医学杂志,2019,(4):515.
　MA Mingming,LI Fang,YANG Hui,et al.Effect of tiotropium bromide powder and nonpharmacological rehabilitation program on BODE index in patients with chronic obstructive pulmonary disease[J].,2019,(11):515.
[2]朱小川.血小板/淋巴细胞比值预测慢性阻塞性肺疾病急性加重期〖JZ〗伴有早期肾功能损害临床价值[J].陕西医学杂志,2019,(10):1353.
　ZHU Xiaochuan..Clinical value of platelet/lymphocyte ratio in predicting early renal impairment〖JZ〗 in acute exacerbation of chronic obstructive pulmonary disease[J].,2019,(11):1353.
[3]张军营.吸烟对慢阻肺患者血清S100A8/A9、8异前列腺素F2α、组织蛋白酶S及其它炎症因子表达水平的影响*[J].陕西医学杂志,2019,(11):1426.
[4]焦文妤,尚家璐,李树霞△.慢性阻塞性肺疾病患者血清补体C1q肿瘤坏死因子相关蛋白5与肺功能及炎症反应相关性研究[J].陕西医学杂志,2019,(12):1616.
[5]陈辅萍.慢性阻塞性肺疾病并发感染性肺炎危险因素分析[J].陕西医学杂志,2020,49(1):102.
[6]薛佩妮,冯敏娟△,王惠琴.噻托溴铵粉联合糖皮质激素治疗慢性阻塞性肺疾病疗效及对患者生活质量评分的影响*[J].陕西医学杂志,2020,49(2):216.
　XUE Peini,FENG Minjuan,WANG Huiqin..Efficacy of tiotropium bromide combined with glucocorticoids in the treatment of COPD and its effect on patients' quality of life score[J].,2020,49(11):216.
[7]彭 娜,卢美君,康马飞△,等.白藜芦醇抑制重组人白细胞介素11促进肺腺癌转移灶形成实验研究*[J].陕西医学杂志,2020,49(4):401.
　PENG Na,LU Meijun,KANG Mafei,et al.Experimental study on resveratrol inhibited the formation of metastatic lesions of lung adenocarcinoma promoted by recombinant human interleukin 11[J].,2020,49(11):401.
[8]焦文妤,高爱华△.血清可溶性髓系细胞触发受体-1与临床肺部感染评分在慢性阻塞性肺疾病呼吸机相关性肺炎早期诊断及预后判断中的效能研究[J].陕西医学杂志,2020,49(12):1630.[doi:DOI:10.3969/j.issn.1000-7377.2020.12.024]
　JIAO Wenyu,GAO Aihua..Effectiveness of sTREM-1 and CPIS in early diagnosis and prognosis judgment of ventilator-associated pneumonia in COPD[J].,2020,49(11):1630.[doi:DOI:10.3969/j.issn.1000-7377.2020.12.024]
[9]张卓红,赵拖利.慢性阻塞性肺疾病大鼠血液、支气管灌洗液中水通道蛋白及p-c-Jun-N-末端激酶表达情况实验研究[J].陕西医学杂志,2021,50(7):776.[doi:DOI:10.3969/j.issn.1000-7377.2021.07.002]
　ZHANG Zhuohong,ZHAO Tuoli.Expression of AQP-1 and p-JNK in blood and bronchial lavage fluid of rats with COPD[J].,2021,50(11):776.[doi:DOI:10.3969/j.issn.1000-7377.2021.07.002]
[10]顾 勇,杨 艳,符 翠,等.白藜芦醇对结肠癌恶性生物学行为的抑制作用研究[J].陕西医学杂志,2021,50(9):1072.[doi:DOI:10.3969/j.issn.1000-7377.2021.09.008]
　GU Yong,YANG Yan,FU Cui,et al.Inhibitory effect of resveratrol on malignant biological behavior of colon cancer[J].,2021,50(11):1072.[doi:DOI:10.3969/j.issn.1000-7377.2021.09.008]

备注/Memo

备注/Memo:
基金项目:陕西省重点研发计划项目(2022SF-230)
更新日期/Last Update: 2023-11-06