[1]陈 庆,李乙航,赵金燕,等.微小核糖核酸34a与沉默信息调节因子1在宫颈癌组织中的表达及相关性研究[J].陕西医学杂志,2023,52(8):1089-1092,1105.[doi:DOI:10.3969/j.issn.1000-7377.2023.08.034]
　CHEN Qing,LI Yihang,ZHAO Jinyan,et al.Expression and association analysis of miR-34a and SIRT1 in cervical cancer tissues[J].,2023,52(8):1089-1092,1105.[doi:DOI:10.3969/j.issn.1000-7377.2023.08.034]
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微小核糖核酸34a与沉默信息调节因子1在宫颈癌组织中的表达及相关性研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年8期
页码:
1089-1092,1105
栏目:
临床病理
出版日期:
2023-08-05

文章信息/Info

Title:
Expression and association analysis of miR-34a and SIRT1 in cervical cancer tissues
作者:
陈 庆1; 李乙航2; 赵金燕1; 吴晓玲1; 谢秀英1
(1.西安交通大学第二附属医院,陕西 西安 710004; 2.长春生物制品研究所有限责任公司,吉林 长春 130012)
Author(s):
CHEN Qing; LI Yihang; ZHAO Jinyan; WU Xiaoling; XIE Xiuying
(The Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710004,China)
关键词:
微小核糖核酸34a;  沉默信息调节因子1;  宫颈癌;  相关性;  荧光定量PCR;  免疫组化
Keywords:
microRNA-34a;  Silent information regulator 1;  Cervical cancer;  Association;  Fluorogenic quantitative PCR;  Immunohistochemistry
分类号:
R 737.33
DOI:
DOI:10.3969/j.issn.1000-7377.2023.08.034
文献标志码:
A
摘要:
目的:探讨微小核糖核酸34a(miR-34a)及沉默信息调节因子1(SIRT1)在宫颈癌组织中的表达及其相关性。方法:收集住院患者标本,采用RT-qPCR法检测miR-34a在正常宫颈、宫颈癌组织中的表达; 免疫组化法检测SIRT1在宫颈癌组织及正常宫颈组织中的表达,分析miR-34a及SIRT1表达与宫颈癌患者临床病理参数的关系,并作Spearman相关分析。结果:miR-34a在宫颈癌组织中的表达明显低于正常宫颈组织(P<0.05),且miR-34a在宫颈癌组织的表达与肿瘤分化程度显著相关(均P<0.05); 免疫组化显示SIRT1在宫颈癌组织中的表达高于正常宫颈组织,其阳性表达率分别为84%与20%,差异具有统计学意义(P<0.05); Spearman分析显示,SIRT1与miR-34a在宫颈癌组织中的表达呈负相关(r=-0.327,P<0.05)。结论:miR-34a在宫颈癌的发生中起重要作用,这为宫颈癌临床治疗提供了一个潜在靶点。
Abstract:
Objective:To investigate the expression of and association of microRNA-34a(miR-34a)and silent information regulator 1(SIRT1)in cervical cancer tissues.Methods:Samples of inpatients were collected,and the expression of miR-34a in normal cervix and cervical cancer tissues was detected by RT-qPCR.The expression of SIRT1 in cervical cancer tissues was detected by immunohistochemistry,and the relationship between the expression of miR-34a,SIRT1 and clinicopathologic parameters of patients with cervical cancer was analyzed.Spearman correlation analysis was performed.Results:The expression of miR-34a in cervical cancer tissues was significantly lower than that in normal cervix(P<0.05).The expression of miR-34a in cervical cancer tissues was significantly correlated with the degree of tumor differentiation(P<0.05).Immunohistochemistry showed that the expression of SIRT1 in cervical cancer tissues was higher than that in normal cervix(P<0.05),and the positive expression rates were 84% and 20%,respectively.Spearman analysis showed that SIRT1 was negatively correlated with miR-34a expression in cervical cancer tissues(r =-0.327,P<0.05).Conclusion:MiR-34a plays an important role in the development of cervical cancer,which provides a potential target for clinical treatment of cervical cancer.

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备注/Memo

备注/Memo:
基金项目:陕西省重点研发计划项目(2023YBSF217); 陕西省自然科学基金资助项目(2017JM8153)
更新日期/Last Update: 2023-08-07