[1]王秋菊,郑丽雅,申 岩,等.微小RNA-335-5p靶向轴突导向因子3B对子宫内膜癌细胞增殖、凋亡的影响[J].陕西医学杂志,2023,52(7):798-802.[doi:DOI:10.3969/j.issn.1000-7377.2023.07.005]
　WANG Qiuju,ZHENG Liya,SHEN Yan,et al.Effects of miR-335-5p targeting SEMA3B on proliferation and apoptosis of endometrial cancer cells[J].,2023,52(7):798-802.[doi:DOI:10.3969/j.issn.1000-7377.2023.07.005]
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微小RNA-335-5p靶向轴突导向因子3B对子宫内膜癌细胞增殖、凋亡的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年7期
页码:
798-802
栏目:
基础研究
出版日期:
2023-07-05

文章信息/Info

Title:
Effects of miR-335-5p targeting SEMA3B on proliferation and apoptosis of endometrial cancer cells
作者:
王秋菊1; 郑丽雅1; 申 岩2; 王园园1
(1.保定市第一中心医院,河北 保定 071000; 2.保定市安国市医院,河北 保定 071200)
Author(s):
WANG Qiuju; ZHENG Liya; SHEN Yan; WANG Yuanyuan
(Baoding No.1 Central Hospital,Baoding 071000,China)
关键词:
微小RNA-335-5p;  轴突导向因子3B;  子宫内膜癌;  细胞增殖;  细胞凋亡;  靶点
Keywords:
miR-335-5p;  Semaphorins 3B;  Endometrial cancer;  Cell proliferation;  Apoptosis;  Target spot
分类号:
R 742
DOI:
DOI:10.3969/j.issn.1000-7377.2023.07.005
文献标志码:
A
摘要:
目的:探讨微小RNA-335-5p(miR-335-5p)靶向轴突导向因子3B(SEMA3B)对子宫内膜癌细胞(Ishikawa细胞)增殖、凋亡的影响及其作用机制。方法:细胞转染分为miR-335-5p NC组、miR-335-5p inhibitor组、pcDNA组、pcDNA-SEMA3B组、miR-335-5p NC+si NC组、miR-335-5p inhibitor+si NC组、miR-335-5p NC+si SEMA3B组、miR-335-5p inhibitor+si SEMA3B组,未转染的Ishikawa细胞作为对照组。生物信息学预测和双荧光素酶法验证miR-335-5p和SEMA3B的靶向关系; 实时荧光定量聚合酶链式反应(qRT-PCR)检测细胞中miR-335-5p和SEMA3B表达; MTT法检测细胞增殖; 流式细胞术检测细胞凋亡; 蛋白质免疫印迹(WB)检测SEMA3B蛋白表达。结果:miR-335-5p和SEMA3B存在靶向关系,并且在子宫内膜癌组织和Ishikawa细胞中检测到miR-335-5p的高表达和SEMA3B的低表达。与miR-335-5p NC组比较,miR-335-5p inhibitor组细胞增殖率显著降低、细胞凋亡率显著升高(均P<0.05)。与pcDNA组比较,pcDNA-SEMA3B组细胞增殖率显著降低、细胞凋亡率显著升高(均P<0.05)。与miR-335-5p NC+si NC组比较,miR-335-5p inhibitor+si NC组细胞增殖率显著降低、细胞凋亡率显著升高(均P<0.05),miR-335-5p NC+si SEMA3B组细胞增殖率显著升高、细胞凋亡率降低(均P<0.05)。与miR-335-5p NC+si SEMA3B比较,miR-335-5p inhibitor+si SEMA3B组细胞增殖率降低、细胞凋亡率升高(均P<0.05)。与miR-335-5p inhibitor+si NC组比较,miR-335-5p inhibitor+si SEMA3B组细胞增殖率升高、细胞凋亡率降低(均P<0.05)。结论:抑制miR-335-5p可靶向激活SEMA3B表达,从而促进子宫内膜癌细胞的凋亡、抑制癌细胞增殖。
Abstract:
Objective:To investigate the effects of miR-335-5p targeting semaphorins 3B(SEMA3B)on the proliferation and apoptosis of endometrial cancer cells(Ishikawa cells)and its mechanism.Methods:Cells were transfected and divided into miR-335-5p NC group,miR-335-5p inhibitor group,pcDNA group,pcDNA-SEMA3B group,miR-335-5p NC+si NC group,miR-335-5p inhibitor+si NC group,miR-335-5p NC+si SEMA3B group,miR-335-5p inhibitor+si SEMA3B group,and the untransfected Ishikawa cells served as the control group.Bioinformatics prediction and dual luciferase method were used to verify the targeting relationship between miR-335-5p and SEMA3B.The qRT-PCR was used to detect the expression of miR-335-5p and SEMA3B in cells.MTT method was used to detect cell proliferatio.Flow cytometry was used to detect cell apoptosis.Western blot(WB)was used to detect the expression of SEMA3B protein.Results:There was a targeting relationship between miR-335-5p and SEMA3B,and high expression of miR-335-5p and low expression of SEMA3B were detected in endometrial cancer tissues and Ishikawa cells.Compared with the miR-335-5p NC group,the cell proliferation rate of the miR-335-5p inhibitor group was significantly reduced,and the cell apoptosis rate was significantly increased(all P<0.05).Compared with the pcDNA group,the cell proliferation rate of the pcDNA-SEMA3B group was significantly reduced,and the cell apoptosis rate was significantly increased(all P<0.05).Compared with miR-335-5p NC+si NC group,the cell proliferation rate of miR-335-5p inhibitor+si NC group was significantly reduced,and the apoptosis rate was significantly increased(all P<0.05),the cell proliferation rate in the miR-335-5p NC+si SEMA3B group was significantly increased,and the cell apoptosis rate was decreased(all P<0.05).Compared with the miR-335-5p NC+si SEMA3B group,the cell proliferation rate in the miR-335-5p inhibitor+si SEMA3B group was decreased,and the apoptosis rate was increased(all P<0.05).Compared with the miR-335-5p inhibitor+si NC group,the cell proliferation rate in the miR-335-5p inhibitor+si SEMA3B group was increased,and the apoptosis rate was decreased(all P<0.05).Conclusion:Inhibition of miR-335-5p can target and activate the expression of SEMA3B,thereby promoting the apoptosis of endometrial cancer cells and inhibiting the proliferation of cancer cells.

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备注/Memo

备注/Memo:
基金项目:河北省医学科学研究计划项目(20220315); 河北省保定市科技计划项目(2141ZF252)
更新日期/Last Update: 2023-07-05