[1]黎珊珊,区岛良,张 梅,等.紫草素对糖尿病周围神经病变大鼠的治疗作用及对腺苷酸活化蛋白激酶/核因子E2相关因子2信号通路的影响[J].陕西医学杂志,2023,52(6):683-687.[doi:DOI:10.3969/j.issn.1000-7377.2023.06.010]
　LI Shanshan,OU Daoliang,ZHANG Mei,et al.Therapeutic effect of shikonin on diabetes peripheral neuropathy rats and its effect on AMPK/Nrf2 signaling pathway[J].,2023,52(6):683-687.[doi:DOI:10.3969/j.issn.1000-7377.2023.06.010]
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紫草素对糖尿病周围神经病变大鼠的治疗作用及对腺苷酸活化蛋白激酶/核因子E2相关因子2信号通路的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年6期
页码:
683-687
栏目:
基础研究
出版日期:
2023-06-05

文章信息/Info

Title:
Therapeutic effect of shikonin on diabetes peripheral neuropathy rats and its effect on AMPK/Nrf2 signaling pathway
作者:
黎珊珊; 区岛良; 张 梅; 谢桃梅
(儋州市人民医院内分泌科,海南 儋州 571700)
Author(s):
LI Shanshan; OU Daoliang; ZHANG Mei; XIE Taomei
(Department of Endocrinology,Danzhou People's Hospital,Danzhou 571700,China)
关键词:
糖尿病周围神经病变;  紫草素;  腺苷酸活化蛋白激酶/核因子E2相关因子2信号通路;  坐骨神经;  炎症反应;  氧化应激
Keywords:
Diabetic peripheral neuropathy;  Shikonin;  AMP-activated potein kinase/nuclear factor E2-related factor 2 signaling pathway;  Sciatic nerve;  Inflammatory response;  Oxidative stress
分类号:
R 587.2
DOI:
DOI:10.3969/j.issn.1000-7377.2023.06.010
文献标志码:
A
摘要:
目的:探究紫草素对糖尿病周围神经病变(DPN)大鼠的治疗作用及对腺苷酸活化蛋白激酶(AMPK)/核因子E2相关因子2(Nrf2)信号通路的影响。方法:构建DPN模型,将建模成功的48只大鼠随机分为模型组、紫草素低剂量组、紫草素高剂量组和甲钴胺片组,每组12只。另取12只大鼠作为对照组。紫草素低、高剂量组大鼠分别给予12.5、25 mg/kg紫草素灌胃,甲钴胺片组大鼠给予0.14 mg/kg甲钴胺片灌胃,对照组和模型组大鼠给予等体积0.9%氯化钠溶液灌胃,每日给药1次,连续8周。观察各组大鼠坐骨神经病理学变化。检测大鼠坐骨神经感觉神经传导速度(SNCV)和运动神经传导速度(MNCV),血清肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β,坐骨神经超氧化物歧化酶(SOD)、丙二醛(MDA)、AMPK、Nrf2 mRNA和蛋白水平。结果:对照组大鼠坐骨神经结构正常; 模型组大鼠坐骨神经髓鞘变薄,出现白色结缔物质,间隙加宽,可见明显炎症细胞浸润; 紫草素低、高剂量组大鼠坐骨神经病变程度依次减轻; 甲钴胺片组和紫草素高剂量组大鼠坐骨神经病理学变化无明显差异。与对照组比较,坐骨神经SNCV和MNCV、血清SOD水平以及坐骨神经AMPK、Nrf2 mRNA和蛋白水平显著降低,血清TNF-α、IL-6及坐骨神经MDA水平显著升高(均P<0.05)。与模型组比较,坐骨神经SNCV和MNCV、血清SOD水平以及坐骨神经AMPK、Nrf2 mRNA和蛋白水平依次升高,血清TNF-α、IL-6及坐骨神经MDA水平依次降低(均P<0.05)。甲钴胺片组和紫草素高剂量组上述各项指标比较差异无统计学意义(均P>0.05)。结论:紫草素能抑制DPN大鼠炎症反应和氧化应激,保护大鼠坐骨神经,其机制可能与激活AMPK/ Nrf2信号通路有关。
Abstract:
Objective:To investigate the therapeutic effect of shikonin on diabetic peripheral neuropathy(DPN)rats and its effect on AMP-activated potein kinase(AMPK)/nuclear factor E2-related factor 2(Nrf2)signaling pathway.Methods:A DPN model was established,and 48 rats successfully modeled were randomly divided into model group,shikonin low-dose group,shikonin high-dose group and mecobalamine tablet group with 12 rats in each group.Another 12 rats were selected as control group.Shikonin low- and high-dose groups were given 12.5 and 25 mg/kg shikonin intragastric administration,respectively; mecobalamine tablet group was given 0.14 mg/kg mecobalamine tablet intragastric administration; control group and model group were given equal volume 0.9% sodium chloride solution intragastric administration,once a day,for consecutive 8 weeks.The sciatic neuropathological changes of rats in each group were observed.Sensory nerve conduction velocity(SNCV)and motor nerve conduction velocity(MNCV)of sciatic nerve,serum TNF-α and IL-1β,levels of SOD,MDA,AMPK,Nrf2 mRNA and protein in sciatic nerve were detected.Results:The sciatic nerve structure of rats in control group was normal; in model group,the sciatic nerve boot became thinner,white connective matter appeared,the gap widened,obvious inflammatory cell infiltration was visible; the degree of sciatic neuropathy of rats in shikonin low- and high-dose groups decreased in turn; there was no significant difference in the pathological changes of the sciatic nerve between the mecobalamin tablet group and the shikonin high-dose group.Compared with control group,sciatic nerve SNCV and MNCV,serum SOD level,sciatic nerve AMPK,Nrf2 mRNA and protein levels were significantly decreased,while serum TNF-α,IL-6 and sciatic nerve MDA levels were significantly increased(all P<0.05).Compared with model group,sciatic nerve SNCV and MNCV,serum SOD level,sciatic nerve AMPK,Nrf2 mRNA and protein levels were increased successively,while serum TNF-α,IL-6 and sciatic nerve MDA level were decreased successively(all P<0.05).There was no statistical significance in the above indexes between mecobalamine tablet group and shikotin high-dose group(all P>0.05).Conclusion:Shikotin can inhibit inflammatory response and oxidative stress,and protect sciatic nerve in DPN rats.The mechanism may be related to the activation of AMPK/ Nrf2 signaling pathway.

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相似文献/References:

[1]李艳飞,王 晶,陈定章,等.下肢神经剪切波弹性成像对不同程度糖尿病周围神经病变的诊断效能分析[J].陕西医学杂志,2022,51(9):1102.[doi:DOI:10.3969/j.issn.1000-7377.2022.09.013]
　LI Yanfei,WANG Jing,CHEN Dingzhang,et al.Diagnostic efficiency of lower limb nerve shear wave elastography in diabetic peripheral neuropathy of different degrees[J].,2022,51(6):1102.[doi:DOI:10.3969/j.issn.1000-7377.2022.09.013]

备注/Memo

备注/Memo:
基金项目:海南省卫生健康行业科研项目(20A200537)
更新日期/Last Update: 2023-06-05