[1]张素冰,高 辉,赵滨滨,等.罗哌卡因对骨肉瘤细胞增殖、侵袭及雷帕霉素靶蛋白/缺氧诱导因子1α信号通路的影响[J].陕西医学杂志,2023,52(6):666-670.[doi:DOI:10.3969/j.issn.1000-7377.2023.06.007]
　ZHANG Subing,GAO Hui,ZHAO Binbin,et al.Effects of ropivacaine on proliferation,invasion and mTOR/HIF-1α signaling pathway of osteosarcoma cells[J].,2023,52(6):666-670.[doi:DOI:10.3969/j.issn.1000-7377.2023.06.007]
点击复制

罗哌卡因对骨肉瘤细胞增殖、侵袭及雷帕霉素靶蛋白/缺氧诱导因子1α信号通路的影响
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年6期
页码:
666-670
栏目:
基础研究
出版日期:
2023-06-05

文章信息/Info

Title:
Effects of ropivacaine on proliferation,invasion and mTOR/HIF-1α signaling pathway of osteosarcoma cells
作者:
张素冰1; 高 辉2; 赵滨滨1; 张丹琦1
(1.黑龙江中医药大学附属第一医院麻醉手术科,黑龙江 哈尔滨 150040; 2.黑龙江中医药大学附属第三医院麻醉手术科,黑龙江 哈尔滨 150040)
Author(s):
ZHANG Subing; GAO Hui; ZHAO Binbin; ZHANG Danqi
(Department of Anesthesiology and Surgery,First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150040,China)
关键词:
骨肉瘤;  罗哌卡因;  增殖;  侵袭;  雷帕霉素靶蛋白;  缺氧诱导因子1α
Keywords:
Osteosarcoma;  Ropivacaine;  Proliferation;  Invasion;  Mammalian target of rapamycin;  Hypoxia inducible factor 1α
分类号:
R 738.1
DOI:
DOI:10.3969/j.issn.1000-7377.2023.06.007
文献标志码:
A
摘要:
目的:探究罗哌卡因对骨肉瘤细胞增殖、侵袭及雷帕霉素靶蛋白(mTOR)/缺氧诱导因子1α(HIF-1α)信号通路的影响。方法:将MG63细胞分为对照组、罗哌卡因低浓度组、罗哌卡因中浓度组、罗哌卡因高浓度组。采用平板克隆法测定MG63细胞平板克隆率,采用免疫组化法测定MG63细胞Ki-67表达,采用Transwell法测定MG63细胞侵袭、迁移能力,采用Western blot法测定MG63细胞基质金属蛋白酶2(MMP-2)、MMP-9、mTOR、HIF-1α蛋白表达。结果:与对照组比较,罗哌卡因低、中、高浓度组MG63细胞克隆形成率、Ki-67阳性率、侵袭细胞数、迁移细胞数以及MMP-2、MMP-9、mTOR、HIF-1α蛋白表达降低(均P<0.05)。与罗哌卡因低浓度组比较,罗哌卡因中、高浓度组MG63细胞克隆形成率、Ki-67阳性率、侵袭细胞数、迁移细胞数以及MMP-2、MMP-9、mTOR、HIF-1α蛋白表达降低(均P<0.05)。与罗哌卡因中浓度组比较,罗哌卡因高浓度组MG63细胞克隆形成率、Ki-67阳性率、侵袭细胞数、迁移细胞数以及MMP-2、MMP-9、mTOR、HIF-1α蛋白表达降低(均P<0.05)。结论:罗哌卡因可抑制MG63细胞增殖、侵袭、迁移,并可抑制mTOR/HIF-1α通路的激活。
Abstract:
Objective:To investigate the effects of ropivacaine on the proliferation,invasion and mammalian target of rapamycin(mTOR)/hypoxia-inducible factor 1α(HIF-1α)signaling pathway of osteosarcoma cells.Methods:MG63 cells were grouped into control group,low-concentration ropivacaine group,medium-concentration ropivacaine group,and high-concentration ropivacaine group.Plate cloning method was applied to determine the plate cloning rate of MG63 cells.Immunohistochemistry was used to determine the expression of Ki-67 in MG63 cells.Transwell method was applied to determine the invasion and migration abilities of MG63 cells.Western blot was applied to determine the protein expressions of Matrix metalloproteinase 2(MMP-2),MMP-9,mTOR and HIF-1α in MG63 cells.Results:Compared with the control group,the colony formation rate,the positive rate of Ki-67,the number of invasive cells,the number of migrating cells,the protein expressions of MMP-2,MMP-9,mTOR and HIF-1α decreased in the low-,medium- and high-concentration ropivacaine group(all P<0.05).Compared with the low-concentration ropivacaine group,the colony formation rate,the positive rate of Ki-67,the number of invasive cells,the number of migrating cells,the protein expressions of MMP-2,MMP-9,mTOR and HIF-1α decreased in the medium- and high-concentration ropivacaine group(all P<0.05).Compared with the medium-concentration ropivacaine group,the colony formation rate,the positive rate of Ki-67,the number of invasive cells,the number of migrating cells,the protein expressions of MMP-2,MMP-9,mTOR and HIF-1α decreased in the high-concentration ropivacaine group(all P<0.05).Conclusion:Ropivacaine can inhibit the proliferation,invasion and migration of MG63 cells,and can inhibit the activation of mTOR/HIF-1α pathway.

参考文献/References:

[1] 李 晨,程联智,洪国勇,等.牛蒡子苷元对骨肉瘤细胞增殖和侵袭影响研究[J].陕西中医,2019,40(4):415-417.
[2] 李正华,纪 涛,任建政,等.穗花杉双黄酮对骨肉瘤细胞增殖及周期相关蛋白的影响[J].陕西医学杂志,2022,51(5):534-538.
[3] 张开亮,韩 康,张 勇,等.环状RNA hsa_circ_103809在骨肉瘤中的表达及生物学作用实验研究[J].陕西医学杂志,2021,50(3):259-263.
[4] Heuvel S,Wal S,Bronkhorst EM,et al.Acute cytokine response during breast cancer surgery:Potential role of dexamethasone and lidocaine and relationship with postoperative pain and complications-analysis of three pooled pilot randomized controlled trials[J].J Pain Res,2020,13:1243-1254.
[5] Celik F,Duran T.Effects of fentanyl on pancreatic cancer cell proliferation and cancer stem cell differentiation[J].Cell Mol Biol,2019,65(7):21-25.
[6] 朱 婧,张世平,张文娟,等.盐酸罗哌卡因对胃癌MGC-803细胞增殖和凋亡的作用[J].现代肿瘤医学,2020,28(8):1261-1265.
[7] 苏华华,王艳华.PI3K/AKT/mTOR信号通路在骨肉瘤中的作用[J].生命的化学,2021,41(5):8964-8971.
[8] Liu L,Zhao X,Zou H,et al.Hypoxia promotes gastric cancer malignancy partly through the HIF-1α dependent transcriptional activation of the long non-coding RNA GAPLINC[J].Front Physiol,2016,7:420.
[9] Huang X,Zhou W,Zhang Y.Transcription factor YY1 enhances the stemness of lung cancer cells by stabilizing hypoxia factor HIF-1α under a hypoxic microenvironment[J].Environ Toxicol,2020,36(1):114-122.
[10] Ma P,Xing M,Han L,et al.High PDL1 expression drives glycolysis via an Akt/mTOR/HIF1α axis in acute myeloid leukemia[J].Oncol Rep,2020,43(3):999-1009.
[11] 石宇雄,黄永明,张 娴,等.骨癌通泰方对人成骨肉瘤耐药细胞R-KHOS MRP基因表达的影响[J].陕西中医,2008,29(11):1552-1553.
[12] 赵健衡,张帮健,王 丽,等.舒芬太尼通过调控miR-410-3p/TIAM1轴影响宫颈癌细胞增殖、迁移和侵袭的机制研究[J].中国药师,2021,24(7):267-272.
[13] 文放放,李熊刚,梁红涛.丙泊酚通过上调miR-133a抑制乳腺癌细胞增殖和侵袭的机制研究[J].河北医药,2020(17):2594-2597,2601.
[14] 张顺吉,朱洪宽,李 超,等.异丙酚通过上调miR-520a-3p抑制Wntβ-catenin信号通路调控乳腺癌细胞[J].西部医学,2021,33(5):676-681.
[15] 洪 蕾,张琳玲,李玉莲.罗哌卡因复合舒芬太尼在潜伏期硬膜外分娩镇痛中的疗效观察[J].临床麻醉学杂志,2020,36(5):497-498.
[16] 马 燕,李仲然,马开喜,等.右美托咪定复合罗哌卡因腹横肌平面阻滞对老年患者结直肠癌根治术后早期恢复质量的影响[J].临床麻醉学杂志,2020,36(2):148-151.
[17] 陈 颖,殷佳晗,顾建华,等.罗哌卡因对人甲状腺癌细胞线粒体呼吸作用的影响[J].临床和实验医学杂志,2019,18(9):916-919.
[18] 张 挚.罗哌卡因对乳腺癌预后相关因素的影响及机制研究[D].郑州:郑州大学,2018.
[19] 王华磊,叶向阳,汤立新.雷帕霉素通过mTORC1/HIF-1α信号调控人骨肉瘤细胞凋亡[J].临床骨科杂志,2020,23(6):899-904.
[20] Rathore R,Tine BV.Targeting one-carbon metabolism requires mTOR inhibition:A new therapeutic approach in osteosarcoma[J].Mol Cell Oncol,2021,8(3):1902250.

相似文献/References:

[1]姚红月,李 岩,陈美雪,等.miR223p靶向负调控eIF4E对儿童骨肉瘤细胞增殖影响的研究[J].陕西医学杂志,2020,49(1):16.
　YAO Hongyue,LI Yan,CHEN Meixue,et al.Effect of miR223p targeting negatively regulated eIF4E on the proliferation of osteosarcoma cells in children[J].,2020,49(6):16.
[2]叶久敏,卿 忠,杨 光,等.不同浓度右美托咪定联合罗哌卡因腘动脉与膝关节后囊间隙阻滞用于全膝关节置换镇痛临床研究[J].陕西医学杂志,2020,49(8):1020.[doi:DOI:10.3969/j.issn.1000-7377.2020.08.027]
　YE Jiumin,QING Zhong,YANG Guang,et al.Clinical study on analgesia of different concentrations of dexmedetomidine combined with ropivacaine for IPACK block in total knee arthroplasty[J].,2020,49(6):1020.[doi:DOI:10.3969/j.issn.1000-7377.2020.08.027]
[3]张 哲,程漠松,庄 鹤,等.骨肉瘤细胞中miR-215-5p和eIF2a靶向关系及临床意义[J].陕西医学杂志,2021,50(7):886.[doi:DOI:10.3969/j.issn.1000-7377.2021.07.031]
　ZHANG Zhe,CHENG Mosong,ZHUANG He,et al.Targeting relationship between miR-215-5p and eIF2a in osteosarcoma cells and its clinical significance[J].,2021,50(6):886.[doi:DOI:10.3969/j.issn.1000-7377.2021.07.031]
[4]杨 帅,刘 婷.子宫切除术中采用复合麻醉对患者围术期镇痛及卵巢功能的影响[J].陕西医学杂志,2022,51(7):803.[doi:DOI:10.3969/j.issn.1000-7377.2022.07.008]
　YANG Shuai,LIU Ting.Effect of compound anesthesia in hysterectomy on perioperative analgesia and ovarian function[J].,2022,51(6):803.[doi:DOI:10.3969/j.issn.1000-7377.2022.07.008]
[5]侯赛楠,王爱荣,丁苏婉.曲马多复合罗哌卡因臂丛神经阻滞对全麻肩关节镜手术患者术后镇痛效果的影响[J].陕西医学杂志,2024,(9):1206.[doi:DOI:10.3969/j.issn.1000-7377.2024.09.011]
　HOU Sainan,WANG Airong,DING Suwan.Effect of tramadol combined with ropivacaine brachial plexus block on postoperative analgesia in patients undergoing arthroscopic shoulder surgery under general anesthesia[J].,2024,(6):1206.[doi:DOI:10.3969/j.issn.1000-7377.2024.09.011]
[6]李 程,关贵超,李 兵,等.骨肉瘤组织锌-α2-糖蛋白1表达及其与患者预后关系[J].陕西医学杂志,2024,(12):1709.[doi:DOI:10.3969/j.issn.1000-7377.2024.12.025]
　LI Cheng,GUAN Guichao,LI Bing,et al.Expression of AZGP1 in osteosarcoma and its relationship with prognosis[J].,2024,(6):1709.[doi:DOI:10.3969/j.issn.1000-7377.2024.12.025]
[7]王海龙,夏丰娜,高学锋,等.罗哌卡因调节沉默信息调控基因1/叉头转录因子1信号通路对白细胞介素-1β诱导的软骨细胞损伤的影响实验研究[J].陕西医学杂志,2025,54(3):314.[doi:DOI:10.3969/j.issn.1000-7377.2025.03.005]
　WANG Hailong,XIA Fengna,GAO Xuefeng,et al.Effect of ropivacaine on interleukin-1β-induced chondrocyte injury by regulating the SIRT1/FOXO1 signaling pathway[J].,2025,54(6):314.[doi:DOI:10.3969/j.issn.1000-7377.2025.03.005]

备注/Memo

备注/Memo:
基金项目:黑龙江省中医药科研项目(ZHY2020-129)
更新日期/Last Update: 2023-06-05