[1]王 剑,魏媛媛,朱利双,等.长链非编码RNA RAB11B-AS1在胶质瘤中的表达及预后价值研究[J].陕西医学杂志,2023,52(4):399-403.[doi:DOI:10.3969/j.issn.1000-7377.2023.04.008]
　WANG Jian,WEI Yuanyuan,ZHU Lishuang,et al.Expression and prognostic value of lncRNA RAB11B-AS1 in glioma[J].,2023,52(4):399-403.[doi:DOI:10.3969/j.issn.1000-7377.2023.04.008]
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长链非编码RNA RAB11B-AS1在胶质瘤中的表达及预后价值研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年4期
页码:
399-403
栏目:
临床研究
出版日期:
2023-04-05

文章信息/Info

Title:
Expression and prognostic value of lncRNA RAB11B-AS1 in glioma
作者:
王 剑1; 魏媛媛2; 朱利双1; 周立敏3
(1.锦州医科大学附属第一医院神经外科一三病区,辽宁 锦州121001; 2.锦州医科大学附属第一医院肿瘤科,辽宁 锦州121001; 3.锦州医科大学附属第一医院感染科,辽宁 锦州121001)
Author(s):
WANG Jian; WEI Yuanyuan; ZHU Lishuang; ZHOU Limin
(Department of Neurosurgery Ward 13,the First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121001,China)
关键词:
脑;  肿瘤;  胶质瘤;  长链非编码RNA RAB11B-AS1;  增殖细胞核抗原;  生存分析
Keywords:
Brain;  Tumor;  Glioma;  LncRNA RAB11B-AS1;  Proliferating cell nuclear antigen;  Survival analysis
分类号:
R 739.41
DOI:
DOI:10.3969/j.issn.1000-7377.2023.04.008
文献标志码:
A
摘要:
目的:检测长链非编码RNA(lncRNA)RAB11B-AS1(RAB11B-AS1)在脑胶质瘤中的表达特征,分析其在不同临床病理特征中的表达差别,探讨其预后价值。方法:应用生信分析数据库(GEPIA)对RAB11B-AS1的表达特征(163例胶质瘤、207例正常脑组织)进行预测。选择68例确诊为脑胶质瘤并行手术治疗的患者作为研究对象,留取术后肿瘤组织,选择68例因脑出血行手术治疗后留取的边缘正常脑组织作为对照。选择人脑胶质瘤细胞株U87和T98G,选择人脑正常胶质细胞株HEB。应用实时荧光定量PCR法(qRT-PCR)检测RAB11B-AS1的表达,应用免疫组化EnVision检测增殖细胞核抗原(PCNA)的表达。结果: GEPIA预测到RAB11B-AS1在胶质瘤中的表达可能升高。脑胶质瘤术后组织中RAB11B-AS1的表达明显高于正常脑组织(P=0.0001)。胶质瘤中RAB11B-AS1表达在不同肿瘤最大径、WHO分级、有无坏死方面比较差异有统计学意义(均P<0.05)。胶质瘤中RAB11B-AS1与PCNA具有正相关性(r=0.62,P=0.0341)。生存分析显示RAB11B-AS1的表达与术后生存时间有关(P=0.041)。胶质瘤细胞株U87和T98G中RAB11B-AS1的表达均明显高于HEB(均P<0.05)。结论:脑胶质瘤组织中RAB11B-AS1的表达升高,与PCNA的表达具有正相关性,术后检测RAB11B-AS1的表达对判断预后可能有一定价值。
Abstract:
Objective:To detect the expression characteristics of lncRNA RAB11B-AS1(RAB11B-AS1)in glioma,analyze the differences on different clinicopathological features,and explore its prognostic value.Methods:The expression characteristics of RAB11B-AS1(163 cases of glioma and 207 cases of normal brain tissue)were predicted by GEPIA.Sixty-eight patients diagnosed with glioma and treated with surgery were selected as study subjects,and postoperative tumor tissues were retained.Sixty-eight cases of marginal normal brain tissue collected after surgical treatment of cerebral hemorrhage were selected as controls.Human glioma cell lines U87 and T98G and human normal glial cell line HEB were selected.The expression of RAB11B-AS1 was detected by qRT-PCR,and proliferating cell nuclear antigen(PCNA)was detected by immunohistochemistry EnVision method.Results:GEPIA observations predicted a possible elevated expression of RAB11B-AS1 in gliomas.The expression of RAB11B-AS1 was significantly higher in glioma tissue than that of normal brain tissue(P=0.0001).Expression of RAB11B-AS1 was different in the maximum diameter and WHO grading,with or without necrosis of glioma(P<0.05).Positive correlation was found between RAB11B-AS1 and PCNA(r=0.62,P=0.0341).RAB11B-AS1 expression was associated with postoperative survival time by survival analysis(P=0.041).The expression of RAB11B-AS1 was significantly higher in both glioma cell lines U87 and T98G than that in HEB(P<0.05).Conclusion:The expression of RAB11B-AS1 was increased in glioma,which was positively correlated with PCNA.Postoperative detection of RAB11B-AS1 expression may have certain value in predicting prognosis.

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备注/Memo

备注/Memo:
基金项目:辽宁省教育科学规划立项课题(JG17DB183)
更新日期/Last Update: 2023-04-06