[1]崔文馨,赵晓辉,高蔚然,等.微小RNA-128-3p靶向调控高尔基体膜蛋白1对肺腺癌细胞增殖的作用[J].陕西医学杂志,2023,52(4):385-389.[doi:DOI:10.3969/j.issn.1000-7377.2023.04.005]
　CUI Wenxin,ZHAO Xiaohui,GAO Weiran,et al.Targeted regulation of GOLM1 by miR-128-3p on proliferation of lung adenocarcinoma cells[J].,2023,52(4):385-389.[doi:DOI:10.3969/j.issn.1000-7377.2023.04.005]
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微小RNA-128-3p靶向调控高尔基体膜蛋白1对肺腺癌细胞增殖的作用
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年4期
页码:
385-389
栏目:
基础研究
出版日期:
2023-04-05

文章信息/Info

Title:
Targeted regulation of GOLM1 by miR-128-3p on proliferation of lung adenocarcinoma cells
作者:
崔文馨1; 赵晓辉2 ; 高蔚然2; 王 娜3
(1.锦州医科大学附属第一医院门诊采血室,辽宁 锦州121000; 2.锦州医科大学附属第一医院肿瘤四病区,辽宁 锦州121000; 3.锦州医科大学附属第一医院日间化疗中心,辽宁 锦州121000)
Author(s):
CUI Wenxin; ZHAO Xiaohui; GAO Weiran; WANG Na
(Outpatient Blood Collection Room,the First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,China)
关键词:
肺腺癌;  细胞系;  微小RNA-128-3p;  高尔基体膜蛋白1;  细胞增殖;  调控
Keywords:
Lung adenocarcinoma;  Cell line;  miR-128-3p;  Golgi membrane protein 1;  Cell proliferation;  Regulation
分类号:
R 734.2
DOI:
DOI:10.3969/j.issn.1000-7377.2023.04.005
文献标志码:
A
摘要:
目的:观察微小RNA-128-3p(miR-128-3p)和高尔基体膜蛋白1(GOLM1)在肺腺癌细胞系中的表达特征,探讨miR-128-3p是否通过介导GOLM1调控肺腺癌细胞的增殖。方法:选择人肺癌细胞系SK-LU-1和A549、人永生化肺上皮细胞系16HBE,对SK-LU-1和A549分别建立miR-128-3p inhibitor组、siRNA-GOLM1组,并分别设立空白对照组,应用Western blot法检测GOLM1和增殖细胞核抗原(PCNA)的表达,应用实时荧光定量PCR法检测miR-128-3p的表达,应用CCK-8检测细胞增殖活性,挽救实验观察小干扰RNA-GOLM1逆转miR-128-3p inhibitor对PCNA表达的影响。结果:肺腺癌细胞系SK-LU-1和A549中miR-128-3p的表达量均明显低于16HBE,GOLM1和PCNA的表达量均明显高于16HBE(均P<0.05)。肺腺癌细胞系SK-LU-1 和A549中,miR-128-3p inhibitior组中GOLM1的表达均明显高于空白对照组(均P<0.05),siRNA-GOLM1组的PCNA的表达量均明显低于空白对照组(均P<0.05),siRNA-GOLM1组在72 h开始细胞增殖活性均低于空白对照组(均P<0.05),均持续至96 h(均P<0.05),挽救实验显示干扰GOLM1可部分逆转miR-128-3p inhibitor对细胞增殖的促进作用(均P<0.05)。结论:肺腺癌细胞系中miR-128-3p低表达、GOLM1高表达,miR-128-3p靶向负调控GOLM1抑制肺腺癌细胞的增殖。
Abstract:
Objective:To observe the expression characteristics of microRNA-128-3p(miR-128-3p)and Golgi membrane protein 1(GOLM1)in lung adenocarcinoma cell lines,and to investigate whether miR-128-3p regulates lung adenocarcinoma cell proliferation by mediating GOLM1.Methods:Human lung cancer cell lines SK-LU-1 and A549 and human immortalized lung epithelial cell line 16HBE were selected.MiR-128-3p inhibitor group and siRNA-GOLM1 group were established respectively for SK-LU-1 and A549,and a blank control group was established respectively.GOLM1 and proliferating cell nuclear antigen(PCNA)were detected by Western blot,and miR-128-3p was detected by qRT-PCR,and the cell proliferation activity was detected by CCK-8.Results:The expression of miR-128-3p were significantly lower in both lung adenocarcinoma cell lines SK-LU-1 and A549 than that of 16HBE(all P<0.05),and the expressions of GOLM1 and PCNA were significantly higher in both lung adenocarcinoma cell lines SK-LU-1 and A549 than that of 16HBE(all P<0.05).GOLM1 was significantly higher in miR-128-3p inhibitior group than that of blank control group of SK-LU-1 and A549(all P<0.05).The expression of PCNA was significantly lower in the siRNA-GOLM1 group than that in the blank control group of SK-LU-1 and A549(all P<0.05).The cell proliferation activity was lower in the siRNA-GOLM1 group than that in the blank control group at 72 hours of SK-LU-1 and A549,and lasted until 96 hours(all P<0.05).The rescue experiments showed that interfering with GOLM1 could partially reverse the promotion effect of miR-128-3p inhibitor on cell proliferation(all P<0.05).Conclusion:MiR-128-3p is lowly expressed and GOLM1 is highly expressed in lung adenocarcinoma cell lines.MiR-128-3p inhibits the proliferation of lung adenocarcinoma cells by targeting GOLM1.

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备注/Memo

备注/Memo:
基金项目:中国医疗手牵手工程委员会-北京医学奖励基金会资助项目(YXJL-2021-1092-0640)
更新日期/Last Update: 2023-04-06