[1]戚新春,易建华.长链非编码RNA BCYRN1对脑胶质瘤大鼠HIF-1α/VEGF信号通路和血管生成的作用机制[J].陕西医学杂志,2023,52(4):376-379.[doi:DOI:10.3969/j.issn.1000-7377.2023.04.003]
　QI Xinchun,YI Jianhua.Mechanism of lncRNA BCYRN1 on HIF-1α/VEGF signaling pathway and angiogenesis in glioma rats[J].,2023,52(4):376-379.[doi:DOI:10.3969/j.issn.1000-7377.2023.04.003]
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长链非编码RNA BCYRN1对脑胶质瘤大鼠HIF-1α/VEGF信号通路和血管生成的作用机制
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年4期
页码:
376-379
栏目:
基础研究
出版日期:
2023-04-05

文章信息/Info

Title:
Mechanism of lncRNA BCYRN1 on HIF-1α/VEGF signaling pathway and angiogenesis in glioma rats
作者:
戚新春1; 易建华2
(1.陕西省肿瘤医院头颈肿瘤科,陕西 西安 710061; 2.西安医学高等专科学校附属医院检验科,陕西 西安 710312)
Author(s):
QI Xinchun; YI Jianhua
(Department of Head and Neck Oncology,Shaanxi Provincial Cancer Hospital,Xi'an 710061,China)
关键词:
长链非编码RNA BCYRN1;  脑胶质瘤;  大鼠;  HIF-1α/VEGF信号通路;  血管生成
Keywords:
lncRNA BCYRN1;  Glioma;  Rats;  HIF-1α/VEGF signaling pathway;  Angiogenesis
分类号:
R 739.41
DOI:
DOI:10.3969/j.issn.1000-7377.2023.04.003
文献标志码:
A
摘要:
目的:探究长链非编码RNA(lncRNA)BCYRN1对脑胶质瘤大鼠的作用和潜在机制,为优化临床治疗方案积累理论基础。方法:制备脑胶质瘤大鼠模型并随机分为三组,每组12只,分别经尾静脉注射lncRNA BCYRN1 siRNA质粒(si-BCYRN1组),lncRNA BCYRN1过表达质粒(OE-BCYRN1组),lncRNA BCYRN1空载质粒(Sham组)。同时选取12只大鼠作为空白对照组(Control组),尾静脉注射等量0.9%氯化钠溶液。比较各组大鼠抑瘤率、缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)蛋白和mRNA表达水平及血管生成相关指标。结果:干预后,OE-BCYRN1组大鼠的瘤重、瘤重/脑重明显低于其他组,且Sham组低于si-BCYRN1组; 与Control组相比,各组的HIF-1α 蛋白和mRNA、VEGF蛋白和mRNA及苏氨酸激酶3(AKT3)蛋白表达升高,OE-BCYRN1组表达最低,si-BCYRN1组最高; 同时,干预后,OE-BCYRN1组大鼠微血管数量明显低于其他组,且Sham组低于si-BCYRN1组,差异均具有统计学意义(均P<0.05)。结论:lncRNA BCYRN1可能通过调控HIF-1α/VEGF信号通路,上调HIF-1α蛋白及mRNA表达,同时下调VEGF蛋白及mRNA表达,减缓瘤组织的生长,并且抑制瘤组织血管生成。
Abstract:
Objective:To explore the effects and potential mechanisms of lncRNA BCYRN1 on glioma rats,and to accumulate theoretical basis for optimizing clinical treatment.Methods:Brain glioma rat models were prepared and randomly divided into 3 groups with 12 rats in each.lncRNA BCYRN1 siRNA plasmid(si-BCYRN1 group)and lncRNA BCYRN1 overexpressed plasmid(OE-BCYRN1 group),lncRNA BCYRN1 empty plasmid(Sham group)were injected through tail vein,respectively.At the same time,12 rats were selected as blank control group,and the same amount of 0.9% sodium chloride solution was injected into the tail vein.The tumor inhibitory rate,HIF-1α,VEGF protein and mRNA expression levels and angiogenesis related indexes were compared in each group.Results:After intervention,the tumor weight,tumor weight/brain weight of OE-BCYRN1 group were significantly lower than those of other groups,and those of Sham group were lower than those of si-BCYRN1 group; compared with control group,HIF-1α protein and mRNA,VEGF protein and mRNA,and AKT3 protein expression were increased,while OE-BCYRN1 group had the lowest expression and si-BCYRN1 group had the highest expression; at the same time,after intervention,the number of microvessels in OE-BCYRN1 group was significantly lower than that in other groups,and that in Sham group was lower than that in si-BCYRN1 group(all P<0.05).Conclusion:lncRNA BCYRN1 may regulate HIF-1α/VEGF signaling pathway,up-regulate HIF-1α protein and mRNA expression,down-regulate VEGF protein and mRNA expression,slow down the growth of tumor tissue,and inhibit the angiogenesis of tumor tissue.

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备注/Memo

备注/Memo:
基金项目:陕西省重点研发计划项目(2021SF-011)
更新日期/Last Update: 2023-04-06