[1]刘彤崴,高丽利,蔡宏宇,等.长链非编码RNA牛磺酸上调基因1在糖尿病肾病中的表达及意义[J].陕西医学杂志,2023,52(3):340-343.[doi:DOI:10.3969/j.issn.1000-7377.2023.03.022]
　LIU Tongwei,GAO Lili,CAI Hongyu,et al.Expression and significance of lncRNA TUG1 in diabetic nephropathy[J].,2023,52(3):340-343.[doi:DOI:10.3969/j.issn.1000-7377.2023.03.022]
点击复制

长链非编码RNA牛磺酸上调基因1在糖尿病肾病中的表达及意义
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年3期
页码:
340-343
栏目:
临床检验
出版日期:
2023-03-05

文章信息/Info

Title:
Expression and significance of lncRNA TUG1 in diabetic nephropathy
作者:
刘彤崴1; 高丽利2; 蔡宏宇3; 王成财4; 傅德望5; 周 盾6; 高 晶7
(1.锦州医科大学附属第一医院护理部,辽宁 锦州 121001; 2.锦州医科大学附属第一医院肾病内科,辽宁 锦州 121001; 3.锦州医科大学附属第一医院心血管内科,辽宁 锦州 121001; 4.锦州医科大学附属第一医院泌尿肿瘤科,辽宁 锦州 121001; 5.锦州医科大学附属第一医院泌尿结石科,辽宁 锦州 121001; 6.锦州医科大学附属第一医院高压氧科,辽宁 锦州 121001; 7.锦州医科大学附属第一医院泌尿外科,辽宁 锦州 121001)
Author(s):
LIU Tongwei; GAO Lili; CAI Hongyu; WANG Chengcai; FU Dewang; ZHOU Dun; GAO Jing
(Department of Nursing,the First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121001,China)
关键词:
糖尿病肾病;  长链非编码RNA;  牛磺酸上调基因1;  临床病理特征;  相关性
Keywords:
Diabetic nephropathy;  Long non-coding RNA;  Taurine up-regulated gene 1;  Clinicopathological characteristics;  Correlation
分类号:
R 587.2
DOI:
DOI:10.3969/j.issn.1000-7377.2023.03.022
文献标志码:
A
摘要:
目的:探究长链非编码RNA牛磺酸上调基因1(lncRNA TUG1)在糖尿病肾病中的表达及意义。方法:选择糖尿病肾病患者65例为观察组,选择同期肾微小病变患者65例为对照组。均留取病变的穿刺活检组织和患者空腹静脉血,应用实时荧光定量PCR检测病变组织和血清中lncRNA TUG1的表达。比较两组患者病变组织和血清lncRNA TUG1表达水平。比较不同临床病理特征糖尿病肾病患者病变组织和血清lncRNA TUG1表达水平。分析观察组患者病变组织与血清lncRNA TUG1表达的相关性。结果:观察组病变组织和血清中lncRNA TUG1表达水平明显低于对照组(均P<0.05)。lncRNA TUG1在不同病变类型、不同硬化肾小球硬化占比患者病变组织和血清中的表达比较差异有统计学意义(均P<0.05),而在不同性别、年龄患者病变组织和血清中的表达比较差异无统计学意义(均P>0.05)。糖尿病肾病患者病变组织与血清lncRNA TUG1表达呈正相关(r=0.693,P=0.021)。结论:糖尿病肾病患者病变组织与血清lncRNA TUG1表达下降且呈正相关,检测血清lncRNA TUG1表达可能对预测病变的形成有一定价值。
Abstract:
Objective:To investigate the expression and significance of long non-coding RNA taurine up-regulated gene 1(lncRNA TUG1)in diabetic nephropathy.Methods:A total of 65 patients with diabetic nephropathy were selected as the observation group,and 65 patients with microscopic renal lesions in the same period were selected as the control group.The lesion biopsy tissues and fasting venous blood of all patients were collected,and the expression of lncRNA TUG1 in the lesion tissue and serum was detected by qRT-PCR.The expression levels of lncRNA TUG1 in lesion tissue and serum were compared between the two groups.The expression levels of lncRNA TUG1 in lesion tissue and serum of diabetic nephropathy patients with different clinicopathological characteristics were compared.The correlation between lesion tissue and serum lncRNA TUG1 expression in the observation group was analyzed.Results:The expression levels of lncRNA TUG1 in the lesion tissue and serum of the observation group were significantly lower than those of the control group(all P<0.05).There were significant differences in the expression of lncRNA TUG1 in lesion tissue and serum of patients with different lesion types and different proportions of sclerotic glomerulosclerosis(all P<0.05),while there were no significant differences in the expression of lncRNA TUG1 in lesion tissue and serum of patients with different gender and age(all P>0.05).There was a positive correlation between the expression of lncRNA TUG1 in lesion tissue and serum in patients with diabetic nephropathy(r=0.693,P=0.021).Conclusion:The expression of lncRNA TUG1 in lesion tissue and serum of patients with diabetic nephropathy is decreased and positively correlated.Detection of serum lncRNA TUG1 expression may have certain value in predicting the formation of diabetic nephropathy.

参考文献/References:

[1] 刘海波,许建秦,李春梦,等.胱抑素C和甘油三酯检测对糖尿病肾病的早期诊断价值研究[J].陕西医学杂志,2021,50(1):96-99.
[2] 屈雅娟,张敬芳,孙 旗,等.灯盏细辛联合依帕司他对糖尿病肾病模型大鼠的治疗作用及机制[J].陕西中医,2022,43(10):1337-1341.
[3] Malik S,Suchal K,Khan SI,et al.Apigenin ameliorates streptozotocin-induced diabetic nephropathy in rats via MAPK-NF-κB-TNF-α and TGF-β1-MAPK-fibronectin pathways[J].Am J Physiol Renal Physiol,2017,313(2):414-422.
[4] 吴 翔,毛盛程,陈 浪.血清长链非编码RNA Rpph1表达对2型糖尿病患者蛋白尿进展的预测价值研究[J].中国现代医学杂志,2022,32(3):16-20.
[5] 邵明玮,卫芳祎,宋 怡,等.长链非编码RNA在糖尿病肾病中的研究进展与展望[J].中华内分泌代谢杂志,2021,37(11):1020-1024.
[6] Yaribeygi H,Atkin SL,Sahebkar A.Interleukin-18 and diabetic nephropathy:A review[J].J Cell Physiol,2019,234(5):5674-5682.
[7] 袁凤英,孙少康,金智生.长非编码RNA NEAT1在糖尿病及其并发症中的分子调控机制[J].中国生物化学与分子生物学报,2022,38(2):159-164.
[8] Senthilkumar GP,Anithalekshmi MS,Yasir M,et al.Role of omentin 1 and IL-6 in type 2 diabetes mellitus patients with diabetic nephropathy[J].Diabetes Metab Syndr,2018,12(1):23-26.
[9] Long J,Badal SS,Ye Z,et al.Long noncoding RNA Tug1 regulates mitochondrial bioenergetics in diabetic nephropathy[J].J Clin Invest,2016,126(11):4205-4218.
[10] Wang F,Gao X,Zhang R,et al.LncRNA TUG1 ameliorates diabetic nephropathy by inhibiting miR-21 to promote TIMP3-expression[J].Int J Clin Exp Pathol,2019,12(3):717-729.
[11] 李春梦,王海峰,卢 佩,等.糖尿病肾病患者D-二聚体、纤维蛋白原、胱抑素C检测及临床意义[J].陕西医学杂志,2020,49(11):1503-1506.
[12] Wang S,Yi P,Wang N,et al.LncRNA TUG1/miR-29c-3p/SIRT1 axis regulates endoplasmic reticulum stress-mediated renal epithelial cells injury in diabetic nephropathy model in vitro[J].PLoS One,2021,16(6):e0252761.
[13] 刘 芳,张立祥,王慧敏,等.养生益肾汤对糖尿病肾病患者血清细胞因子水平及肾功能的影响[J].陕西中医,2022,43(4):476-478.
[14] Yang H,Xie T,Li D,et al.Tim-3 aggravates podocyte injury in diabetic nephropathy by promoting macrophage activation via the NF-κB/TNF-α pathway[J].Mol Metab,2019,23:24-36.
[15] 韩 敬,顾津伊,聂君旭,等.2型糖尿病肾病患者血清miR-27b-3p、miR-342-3p表达特点及临床意义[J].天津医药,2022,50(10):1077-1082.
[16] Lei X,Zhang L,Li Z,et al.Astragaloside Ⅳ/lncRNA-TUG1/TRAF5 signaling pathway participates in podocyte apoptosis of diabetic nephropathy rats[J].Drug Des Devel Ther,2018,12:2785-2793.
[17] Chen Y,Li Z,Chen X,et al.Long non-coding RNAs:From disease code to drug role[J].Acta Pharm Sin B,2021,11(2):340-354.
[18] Xiang E,Han B,Zhang Q,et al.Human umbilical cord-derived mesenchymal stem cells prevent the progression of early diabetic nephropathy through inhibiting inflammation and fibrosis[J].Stem Cell Res Ther,2020,11(1):336.
[19] 卢发菊,王 丽,陈永建.lncRNA TUG1靶向调控miR-199a-3p对高糖诱导的足细胞损伤的作用与机制研究[J].广西医科大学学报,2021,38(12):2294-2299.
[20] 李小丽,王 辉,张庆军.LncRNA TUG1靶向miR-222-3p对肺炎链球菌诱导的人肺泡上皮细胞损伤的影响[J].国际检验医学杂志,2021,42(24):3054-3058.
[21] 王 焕,尤冠巧,郭利芹,等.长链非编码RNA Gm15645对糖尿病肾病足细胞损伤的影响[J].中华医学遗传学杂志,2021,38(10):1017-1020.
[22] 俞元植,尹 敏,陈佳琦,等.circRNA在糖尿病肾病中的研究进展[J].中国实验诊断学,2022,26(10):1565-1569.
[23] Li L,Long J,Mise K,et al.PGC1α is required for the renoprotective effect of lncRNA Tug1 in vivo and links Tug1 with urea cycle metabolites[J].Cell Rep,2021,36(6):109510.

相似文献/References:

[1]袁江红.腹膜透析与血液透析治疗终末期糖尿病肾病患者疗效比较[J].陕西医学杂志,2016,(10):1365.
[2]徐 琳,贾睿博△,宋民喜,等.颗粒蛋白前体与糖尿病肾病关系探讨*[J].陕西医学杂志,2019,(8):1024.
[3]李 明,门 敏△,王丽萍,等.瑞舒伐他汀联合前列地尔治疗早期糖尿病肾病疗效研究*[J].陕西医学杂志,2019,(9):1223.
　LI Ming,MEN Min,WANG Liping,et al.Efficacy of rosuvastatin combined with alprostadil in the 〖JZ〗treatment of early diabetic nephropathy[J].,2019,(3):1223.
[4]徐 琳,贺菲菲△.糖尿病肾病患者血清铁代谢指标与尿蛋白排泄量相关性分析*[J].陕西医学杂志,2019,(10):1281.
　XU Ling,HE Feifei..Correlation between serum iron metabolism index and urine protein 〖JZ〗excretion in patients with diabetic nephropathy[J].,2019,(3):1281.
[5]高旭航,郑玉群,何志杰,等.基因多态性与糖尿病肾病关系研究进展[J].陕西医学杂志,2019,(11):1574.
　GAO Xuhang,ZHENG Yuqun,HE Zhijie,et al.Research progress on relationsship between gene polymorphism and diabetic nephropathy[J].,2019,(3):1574.
[6]张晓丽.血液透析联合血液灌注对糖尿病肾病患者血清空腹血糖、 C反应蛋白、白细胞介素6影响的临床研究[J].陕西医学杂志,2020,49(1):68.
　ZHANG Xiaoli..Effect of hemodialysis and blood perfusion on serum FPG,CRP and IL6 in patients with diabetic nephropathy[J].,2020,49(3):68.
[7]张 菁,姚 蔚,张进安,等.沙格列汀联合前列地尔治疗老年糖尿病肾病临床评价*[J].陕西医学杂志,2020,49(12):1657.[doi:DOI:10.3969/j.issn.1000-7377.2020.12.032]
　ZHANG Jing,YAO Wei,ZHANG Jin'an,et al.Clinical evaluation of saxagliptin combined with alprostadil in treatment of elderly diabetic nephropathy[J].,2020,49(3):1657.[doi:DOI:10.3969/j.issn.1000-7377.2020.12.032]
[8]易亭伍,梁 睿,宿向东.长链非编码RNA SNHG15通过miR-141-3p /KLF9机制调控鼻咽癌细胞增殖的实验研究[J].陕西医学杂志,2021,50(1):10.[doi:DOI:10.3969/j.issn.1000-7377.2021.01.003]
　YI Tingwu,LIANG Rui,SU Xiangdong.Study on long chain non-coding RNA SNHG15 regulating the proliferation of nasopharyngeal carcinoma cells through miR-141-3p/KLF9[J].,2021,50(3):10.[doi:DOI:10.3969/j.issn.1000-7377.2021.01.003]
[9]刘海波,许建秦,李春梦,等.胱抑素C和甘油三酯检测对糖尿病肾病的早期诊断价值研究[J].陕西医学杂志,2021,50(1):96.[doi:DOI:10.3969/j.issn.1000-7377.2021.01.026]
　LIU Haibo,XU Jianqin,LI Chunmeng,et al.Value of cystatin C and triglyceride in early diagnosis of diabetic nephropathy[J].,2021,50(3):96.[doi:DOI:10.3969/j.issn.1000-7377.2021.01.026]
[10]袁 瑾,赵 灿,张渭涛.依普利酮通过靶向微小RNA-192和微小RNA-29a/b/c减轻糖尿病肾病模型大鼠肾损伤机制研究[J].陕西医学杂志,2022,51(3):293.[doi:DOI:10.3969/j.issn.1000-7377.2022.03.007]
　YUAN Jin,ZHAO Can,ZHANG Weitao.Mechanism of eplerenone alleviating renal injury in diabetic nephropathy rat model by targeting miR-192 and miR-29a/b/c[J].,2022,51(3):293.[doi:DOI:10.3969/j.issn.1000-7377.2022.03.007]

备注/Memo

备注/Memo:
基金项目:辽宁省科学技术计划项目(2021-BS-269)
更新日期/Last Update: 2023-03-06