[1]石艳红,邵 英.生物钟相关基因CLOCK及褪黑素受体MT1和MT2基因与2型糖尿病患者肠道菌群及炎症因子相关性分析[J].陕西医学杂志,2023,52(3):335-339.[doi:DOI:10.3969/j.issn.1000-7377.2023.03.021]
　SHI Yanhong,SHAO Ying.Correlation of clock-related gene CLOCK,melatonin receptor MT1 and MT2 genes with intestinal microbiota and inflammatory factors in patients with type 2 diabetes mellitus[J].,2023,52(3):335-339.[doi:DOI:10.3969/j.issn.1000-7377.2023.03.021]
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生物钟相关基因CLOCK及褪黑素受体MT1和MT2基因与2型糖尿病患者肠道菌群及炎症因子相关性分析
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年3期
页码:
335-339
栏目:
临床检验
出版日期:
2023-03-05

文章信息/Info

Title:
Correlation of clock-related gene CLOCK,melatonin receptor MT1 and MT2 genes with intestinal microbiota and inflammatory factors in patients with type 2 diabetes mellitus
作者:
石艳红1; 邵 英2
(1.铜川矿务局中心医院内分泌科,陕西 铜川 727000; 2.空军军医大学空军第九八六医院内分泌科,陕西 西安 710054)
Author(s):
SHI Yanhong; SHAO Ying
(Department of Endocrinology,Tongchuan Mining Bureau Central Hospital,Tongchuan 727000,China)
关键词:
2型糖尿病;  生物钟相关基因;  肠道菌群;  炎症因子;  相关性
Keywords:
Type 2 diabetes mellitus;  Clock-related gene;  Intestinal microbiota;  Inflammatory factor;  Correlation
分类号:
R 587.1
DOI:
DOI:10.3969/j.issn.1000-7377.2023.03.021
文献标志码:
A
摘要:
目的:探讨生物钟基因CLOCK及褪黑素受体MT1和MT2基因与2型糖尿病(T2DM)患者肠道菌群及炎症因子的相关性。方法:选择T2DM患者80例为观察组,选择同期进行健康体检者80例为对照组。检测两组血清CLOCK、MT1和MT2基因水平,以及超敏C反应蛋白(hs-CRP)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和IL-18水平。检测两组粪便中肠杆菌、双歧杆菌、乳杆菌、肠球菌、双歧杆菌/肠杆菌丰度。分析观察组血清CLOCK、MT1和MT2基因水平与T2DM患者肠道菌群及炎症因子的相关性。结果:观察组血清CLOCK、MT1、MT2基因水平明显较对照组低(均P<0.05)。观察组肠杆菌水平明显较对照组高,双歧杆菌、乳杆菌、双歧杆菌/肠杆菌水平明显较对照组低(均P<0.05)。观察组hs-CRP、IL-6、TNF-α、IL-18水平明显较对照组高(均P<0.05)。观察组血清CLOCK、MT1、MT2基因水平与肠杆菌、hs-CRP、IL-6、TNF-α、IL-18水平呈负相关,与双歧杆菌、乳杆菌、双歧杆菌/肠杆菌水平呈正相关(均P<0.05)。结论:血清生物钟相关基因CLOCK、MT1和MT2基因与T2DM患者肠道菌群及炎症因子均有一定相关性,可作为潜在的诊断指标及治疗靶点。
Abstract:
Objective:To investigate the correlation of clock-related gene CLOCK,melatonin receptor MT1 and MT2 genes with intestinal microbiota and inflammatory factors in patients with type 2 diabetes mellitus(T2DM).Methods:Eighty T2DM patients were selected as observation group,and Eighty healthy people for physical examination during the same period were selected as control group.The levels of CLOCK,MT1 and MTT2,hs-CRP,IL-6,TNF-α and IL-18 in serum of the two groups were detected.The abundance of enterobacterium,bifidobacterium,lactobacillus,enterococcus and bifidobacterium/enterobacterium in feces of the two groups were detected.The correlation of CLOCK,MT1 and MT2 genes with intestinal microbiota and inflammatory factors in T2DM patients was analyzed. Results:The levels of CLOCK,MT1 and MT2 genes in the observation group were significantly lower than those in the control group(all P<0.05).The level of enterobacter in the observation group was significantly higher than that in the control group,and the levels of bifidobacterium,lactobacillus and bifidobacterium/enterobacter in the observation group were significantly lower than those in the control group(all P<0.05).The levels of hs-CRP,IL-6,TNF-α and IL-18 in the observation group were significantly higher than those in the control group(all P<0.05).The levels of CLOCK,MT1 and MT2 genes in the observation group were negatively correlated with the levels of enterobacterium,hs-CRP,IL-6,TNF-α and IL-18,and positively correlated with the levels of bifidobacterium,lactobacillus and bifidobacterium/enterobacterium(all P<0.05).Conclusion:The serum clock-related genes CLOCK,MT1 and MT2 have certain correlation with intestinal microbiota and inflammatory factors in T2DM patients,which can be used as potential diagnostic indicators and therapeutic targets.

参考文献/References:

[1] 贾伟平,包玉倩,缪 珩,等.两种门冬胰岛素治疗糖尿病的有效性及安全性比较:多中心、随机、开放、对照试验[J].中华内科杂志,2021,60(12):1148-1156.
[2] 黄 娜,周英凤,李 丽,等.妊娠期糖尿病孕妇血糖管理决策行为及影响因素的研究[J].中华护理杂志,2021,56(9):1312-1317.
[3] 谭楚红,张铭思,杨梦佳,等.肠道菌群和短链脂肪酸代谢紊乱与高血压性脑出血患者转归的相关性[J].国际脑血管病杂志,2021,29(11):837-844.
[4] 聂 丁,赵 澎.肠道菌群与垂体腺瘤免疫微环境关系的研究进展[J].中华神经外科杂志,2021,37(10):1070-1073.
[5] 宋绍霏,侯园园,王云雷,等.异常光周期所致昼夜节律紊乱对大鼠腓肠肌时钟基因和葡萄糖摄取相关基因表达节律的影响[J].中国康复理论与实践,2022,28(5):552-558.
[6] 路兰芳.2型糖尿病患者HPA轴激素水平与糖代谢的关系[J].实用临床医药杂志,2014,18(15):133-134,137.
[7] 陈振山,张耀文,王小明,等.生物钟系统调控机体代谢的分子机制及中药干预研究进展[J].药学学报,2020,55(12):2818-2826.
[8] 余明芳,刘旭光,吴 晓.生物钟基因Bmal1与骨代谢相关性的研究进展[J].实用医学杂志,2020,36(20):2881-2884.
[9] 中华医学会糖尿病学分会.中国2型糖尿病防治指南(2013年版)[J].中华内分泌代谢杂志,2014,30(8):893-942.
[10] 王丹妮,郭晋良,仝立国,等.蛇床子催眠活性组分对PCPA损伤大鼠松果体细胞的保护作用及钟基因表达调控机制[J].中药材,2021,44(1):182-185.
[11] Liu J,Clough SJ,Hutchinson AJ,et al.MT1 and MT2 melatonin receptors:A therapeutic perspective[J].Annual Review of Pharmacology and Toxicology,2016,56:361-383.
[12] Devavry S,Legros C,Brasseurand C,et al.Molecular pharmacology of the mouse melatonin receptors MT1 and MT2[J].European Journal of Pharmacology,2012,677(3):15-21.
[13] 骆丹越,孔敏刚,章 晨,等.初诊高血压患者血清IL-18、hs-CRP、TNF-α水平与颈动脉内中膜厚度的相关性研究[J].浙江医学,2021,43(10):1062-1065,1070.
[14] 金雪锋,王惠庭,张 颖,等.实时荧光PCR法与细菌培养法检测儿童粪便沙门菌的对比研究[J].中国卫生检验杂志,2021,31(16):1960-1962.
[15] 曹 晋,武 易,邵 英,等.老年2型糖尿病患者红细胞分布宽度与心血管自主神经病变相关性研究[J].陕西医学杂志,2021,50(10):1260-1263.
[16] 崔 志,蔡 华,马淑红,等.芪明颗粒联合西药治疗糖尿病周围神经病变临床研究[J].陕西中医,2021,42(3):312-314.
[17] 白小岗,李 霞,贾爱华,等.2型糖尿病男性患者性激素水平与体重指数、糖脂代谢及胰岛素抵抗相关性分析[J].陕西医学杂志,2022,51(9):1086-1089.
[18] 杨川洲,彭艳文,李卫东,等.三才封髓丹加减治疗气阴两虚型2型糖尿病合并汗证疗效研究[J].陕西中医,2022,43(9):1204-1207.
[19] 于 雷,孙 超,张曼旭.灵芝多糖对HepG2细胞诱导的肝癌小鼠肠道菌群及其菌群代谢功能的调节作用[J].实用肝脏病杂志,2021,24(4):476-479.
[20] 付金蓉,关海霞.生物钟和核心钟基因与自身免疫性疾病[J].国际免疫学杂志,2022,45(2):171-175.
[21] 王 霞,李 美,张文奇.CLOCK基因多态性与脑卒中后静脉血栓形成的关系[J].中国老年学杂志,2022,42(12):2879-2883.
[22] 曹 波,张 铭,陈 杨,等.外侧下丘脑与褪黑素降低大鼠觉醒水平的关系及受体机制[J].中华麻醉学杂志,2021,41(3):311-314.

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备注/Memo

备注/Memo:
基金项目:陕西省自然科学基础研究计划项目(2019JM-422)
更新日期/Last Update: 2023-03-06