[1]姜 帆,李春香,李忠原.超声双模态影像技术联合动态增强磁共振检查对大鼠早期肝纤维化的评估价值实验研究[J].陕西医学杂志,2023,52(3):273-277.[doi:DOI:10.3969/j.issn.1000-7377.2023.03.006]
　JIANG Fan,LI Chunxiang,LI Zhongyuan.Evaluation value of ultrasound dual-modality imaging technique combined with DCE-MRI in rats with early hepatic fibrosis[J].,2023,52(3):273-277.[doi:DOI:10.3969/j.issn.1000-7377.2023.03.006]
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超声双模态影像技术联合动态增强磁共振检查对大鼠早期肝纤维化的评估价值实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年3期
页码:
273-277
栏目:
基础研究
出版日期:
2023-03-05

文章信息/Info

Title:
Evaluation value of ultrasound dual-modality imaging technique combined with DCE-MRI in rats with early hepatic fibrosis
作者:
姜 帆1; 2; 李春香1; 李忠原1
(1.锦州医科大学齐齐哈尔医学院附属第一医院研究生培养基地,黑龙江 齐齐哈尔161041; 2.齐齐哈尔市传染病防治院,黑龙江 齐齐哈尔161041)
Author(s):
JIANG Fan; LI Chunxiang; LI Zhongyuan
(Postgraduate Training Base,First Affiliated Hospital of Qiqihar Medical University of Jinzhou Medical University,Qiqihar 161041,China)
关键词:
肝纤维化;  超声弹性成像;  动态增强磁共振;  血清学指标;  HE染色;  大鼠
Keywords:
Hepatic fibrosis;  Ultrasound elastic imaging;  DCE-MRI;  Serologic indexes;  HE staining;  Rats
分类号:
R 575.2
DOI:
DOI:10.3969/j.issn.1000-7377.2023.03.006
文献标志码:
A
摘要:
目的:探讨超声双模态影像技术联合动态增强磁共振(DCE-MRI)检查对大鼠早期肝纤维化(HF)的评估价值。方法:将SD大鼠48只随机分为对照组、M4组、M6组和M8组,每组12只。对照组自由饮食及摄水。模型组(M4、M6、M8组)采用CCl4加乙醇复合法对大鼠进行诱导,M4、M6、M8组分别注射4、6、8周。采用常规超声、超声弹性成像、常规MRI及DCE-MRI对大鼠肝脏进行检查。处死大鼠后,取血清检测谷丙转氨酶(ALT)、谷草转氨酶(AST)、透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原N端肽(PCⅢNP)、Ⅳ型胶原(CⅣ)含量; 取肝组织固定后行HE染色,光镜下观察病理变化。结果:常规超声检查结果显示,对照组大鼠肝脏无异常变化,而模型组大鼠肝脏的形态发生改变。超声弹性成像结果显示,与对照组比较,模型组大鼠肝脏弹性值随着纤维化程度的加重而呈上升趋势(均P<0.05)。常规MRI检查结果显示,对照组大鼠肝脏形态正常,M4、M6、M8组大鼠肝脏出现形态改变,M8组大鼠肝脏变化最为明显。DCE-MRI检查结果显示,随着大鼠HF分级的不断进展,Ktrans、Kep、Ve、iAUC呈依次下降趋势(均P<0.05)。血清学检测结果显示,随着大鼠HF分级的不断进展,ALT、AST、HA、LN、PCⅢNP、CⅣ呈依次升高趋势(均P<0.05)。HE染色结果显示,对照组大鼠肝组织正常,模型组大鼠肝组织出现不同程度的纤维增生。结论:在评估早期肝纤维化的无创诊断技术中,超声双模态影像技术联合动态增强磁共振可有效地提高评估肝纤维化严重程度的能力。
Abstract:
Objective:To investigate the evaluation value of ultrasound dual-modality imaging technique combined with dynamic contrast-enhanced magnetic resonance imaging(DCE-MRI)in rats with early hepatic fibrosis(HF).Methods:Forty-eight SD rats were randomly divided into control group,M4 group,M6 group and M8 group,with 12 rats in each group.The control group was allowed free diet and water.The model group(M4,M6,and M8 groups)was induced with CCl4 and ethanol for 4,6,and 8 weeks,respectively.The livers of rats were examined by conventional ultrasound,ultrasound elastic imaging,conventional MRI,and DCE-MRI.After the rats were executed,serum was taken to determine the levels of ALT,AST,HA,PCⅢNP and CⅣ; liver tissues were fixed and stained with HE,and pathological changes were observed by light microscopy.Results:Conventional ultrasound examination revealed that no abnormal changes in control group,whereas morphological changes were observed in model group.Ultrasound elastic imaging showed that the ultrasound elasticity values increased as the degree of liver fibrosis increased in model group in comparison with control group(all P<0.05).Conventional MRI showed normal morphology in control group,but M4,M6 and M8 groups showed morphological changes that were most pronounced in M8 group.DCE-MRI examination shouwed that Ktrans,Kep,Ve and iAUC decreased as the increase of HF grade(all P<0.05).Serologic tests showed an increasing trend in ALT,AST,HA,LN,PCⅢNP,and CⅣ as the increase of HF grade(all P<0.05).HE staining showed normal liver tissue in control group and varying degrees of fibrosis in model group.Conclusion:Among the noninvasive diagnostic techniques for the assessment of early liver fibrosis,ultrasound dual-modality imaging technique combined with DCE-MRI effectively can improve the ability to assess the severity of liver fibrosis.

参考文献/References:

[1] Zhang ZQ,Yang B,Zou H,et al.ALBI/ST ratio versus FIB-4 and APRI as a predictor of posthepatectomy liver failure in hepatocellular carcinoma patients[J].Medicine,2019,27(4):63-68.
[2] Sarin SK.Asian-pacific clinical practice guidelines on the management of hepatitis B:A 2015 update[J].Hepatology International,2016,10(1):91-98.
[3] 周 翔,顾 达,童 聪,等.鳖甲煎丸对CCl4诱导小鼠肝纤维化的治疗作用机制研究[J].陕西中医,2022,43(2):151-156.
[4] Moreno C,Mueller S,Szabo G.Non-invasive diagnosis and biomarkers in alcohol-related liver disease[J].Journal of Hepatology,2019,70(2):273-283.
[5] 黄艳梅,陈成彩,陈洁华,等.超声弹性成像在无创性肝纤维化诊断中的研究进展[J].影像研究与医学应用,2022,6(11):4-6.
[6] 葛岩松,石鸣琪,孙林娜,等.Gd-EOB-DTPA动态对比增强MRI定量评估大鼠中度肝纤维化价值的实验研究[J].磁共振成像,2022,13(1):7-11.
[7] Liu C,Sun R,Wang J,et al.Combination of DCE-MRI and DWI in predicting the treatment effect of concurrent chemoradiotherapy in esophageal carcinoma[J].Bio Med Research International,2020,2020:2576563.
[8] Asrani SK,Devarbhavi H,Eamath PS.Burden of liver diseases in the world[J].Hepatol,2019,70(2):151-171.
[9] 司 晶,唐 露,李臣诚,等.肝纤维化的发病机制及其治疗药物研究进展[J].药学进展,2021 45(3):205-211.
[10] 雷 玲,闵 珺,刘 锋,等.黄芪对肝纤维化大鼠肝损伤保护作用及机制研究[J].陕西中医,2020,41(9):1192-1196.
[11] 中华医学会超声医学分会,中国研究型医院学会肿瘤介入专业委员会,国家卫生和健康委员会能力建设和继续教育中心超声医学专家委员会,等.肝病超声诊断指南[J].临床肝胆病杂志,2021,37(8):16-34.
[12] 魏 华,马旭艳.彩色多普勒超声半定量评分对早期乙型肝炎肝硬化的诊断价值[J].实用肝脏病杂志,2017,20(1):41-44.
[13] 董常峰,姜 伟.超声弹性成像对肝纤维化的诊断及应用[J].新发传染病电子杂志,2020,5(3):207-211.
[14] 李定富,唐换灵,黄 健,等.血PⅢP、LN、HA水平与慢性肝炎程度、肝纤维化分期及炎症活动度分级的关系[J].临床和实验医学杂志,2022,21(10):1119-1121.
[15] 何 桥,原文聪,樊海宁,等.弹性成像联合血清学诊断慢性乙型肝炎肝纤维化的研究现状[J].临床肝胆病杂志,2021,37(12):2914-2918.
[16] 刘晓敏,孙慧莹,王永福,等.HA、ColⅣ、LN和PⅢNP-P在结缔组织病合并间质性肺病病情评估中的意义[J].天津医药,2021,49(6):617-621.
[17] 王秀丽,姚 鹏.血清透明质酸对慢性病毒性肝炎肝纤维化的诊断价值[J].中国基层医药,2018,25(21):2819-2822.
[18] 蔡碧莲,文亦磊,罗伟生.大鼠肝纤维化实验动物模型的构建[J].陕西医学杂志,2020,49(6):659-662.
[19] 李健明,胡向东,张岩峰,等.剪切波弹性成像的影响因素分析[J].中华医学超声杂志,2019,16(8):565-567.
[20] Liu HF,Wang Q,Du YN,et al.Dynamic contrast-enhanced MRI with Gd-EOB-DTPA for the quantitative assessment of early-stage liver fibrosis induced by carbon tetrachloride in rabbits[J].Magnetic Resonance Imaging,2020,70(7):57-63.
[21] 艾丁丁,罗伟生,蒋云霞.动物肝纤维化模型建立研究进展[J].陕西医学杂志,2020,49(7):907-909.

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备注/Memo

备注/Memo:
基金项目:黑龙江省卫生健康委员会科研课题(20210909040360); 黑龙江省齐齐哈尔市科技计划联合引导项目(LSFGG-2022066)
更新日期/Last Update: 2023-03-06