[1]王 音,杨文娟.卵巢癌移植瘤裸鼠不同时期靶向超声造影参数与癌细胞生长关系研究[J].陕西医学杂志,2022,51(12):1492-1496.[doi:DOI:10.3969/j.issn.1000-7377.2022.12.006]
　WANG Yin,YANG Wenjuan.Relationship between targeted contrast-enhanced ultrasound parameters at different stages and cancer cell growth in nude mice with ovarian cancer xenografts[J].,2022,51(12):1492-1496.[doi:DOI:10.3969/j.issn.1000-7377.2022.12.006]
点击复制

卵巢癌移植瘤裸鼠不同时期靶向超声造影参数与癌细胞生长关系研究
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年12期
页码:
1492-1496
栏目:
基础研究
出版日期:
2022-12-05

文章信息/Info

Title:
Relationship between targeted contrast-enhanced ultrasound parameters at different stages and cancer cell growth in nude mice with ovarian cancer xenografts
作者:
王 音1; 杨文娟2
(1.西安大兴医院超声医学科,陕西 西安 710016; 2.西安秦皇医院超声医学科,陕西 西安 712000)
Author(s):
WANG Yin; YANG Wenjuan
(Department of Ultrasound Medicine,Xi'an Daxing Hospital,Xi'an 710016,China)
关键词:
卵巢癌移植瘤;  靶向超声造影;  普通超声造影;  人卵巢癌SKOV3细胞;  相关性;  裸鼠
Keywords:
Ovarian cancer xenograft;  Targeted contrast-enhanced ultrasound;  Ordinary contrast-enhanced ultrasound;  Human ovarian cancer SKOV3 cells;  Correlation;  Nude mice
分类号:
R 737.31
DOI:
DOI:10.3969/j.issn.1000-7377.2022.12.006
文献标志码:
A
摘要:
目的:探讨卵巢癌移植瘤裸鼠不同时期靶向超声造影参数与癌细胞生长的关系。方法:将人卵巢癌SKOV3细胞移植至50只BALB/c-Nude雌性裸鼠皮下制备卵巢癌移植瘤模型。在造模后第10、20、30、40、50天分别抽取10只裸鼠行超声造影检查。分析超声微泡物理性状及荧光鉴定结果,统计人卵巢癌移植造模结果,比较造模后不同时间点普通与靶向超声造影参数(峰值强度、达峰时间及平均渡越时间)。在不同时间点超声造影完成后处死裸鼠,剥离肿瘤,使用酶联免疫吸附试剂盒检测血管新生分子蛋白血管内皮生长因子受体1(VEGFR1)、酪氨酸激酶受体2(Tie-2)、细胞增殖蛋白[细胞周期蛋白D1(Cyclin D1)和E74样因子5(ELF5)]、细胞侵袭蛋白[胰岛素样生长因子结合蛋白2(IGFBP2)和E-上皮钙黏附素(E-cadherin)]水平。分析靶向超声造影参数与癌细胞生长的相关性。结果:靶向与普通微泡形态、大小比较均无明显差异。荧光显微镜下靶向造影微泡出现明亮红色荧光,普通造影微泡无荧光。造模后不同时间点,普通超声造影的峰值强度、达峰时间、平均渡越时间比较差异无统计学意义(均P>0.05); 而靶向超声造影的峰值强度、达峰时间、平均渡越时间比较差异有统计学意义(均P<0.05)。同一时间点时,靶向超声造影的峰值强度和平均渡越时间高于普通超声造影,达峰时间低于普通超声造影(均P<0.05)。造模后不同时间点,VEGFR1、Tie-2、Cyclin D1、ELF5、IGFBP2、E-cadherin相对表达量比较差异有统计学意义(均P<0.05)。Spearman相关性分析显示,靶向超声造影峰值强度及平均渡越时间与VEGFR1、Tie-2、Cyclin D1、IGFBP2、E-cadherin水平呈正相关,与ELF5呈负相关(均P<0.05); 达峰时间与VEGFR1、Tie-2、Cyclin D1、IGFBP2、E-cadherin水平呈负相关,与ELF5呈正相关(均P<0.05)。结论:不同时期超声靶向造影显影优于普通超声造影,超声造影参数与癌细胞生长有一定的相关性,有助于卵巢癌的早期诊断。
Abstract:
Objective:To investigate the relationship between of targeted contrast-enhanced ultrasound parameters at different stages and cancer cell growth in nude mice with ovarian cancer xenografts.Methods:Human ovarian cancer SKOV3 cells were transplanted into 50 BALB/c-nude female nude mice to prepare ovarian cancer xenograft model.Contrast-enhanced ultrasound was performed in 10 nude mice on days 10,20,30,40 and 50 after modeling,respectively.The physical characteristics and fluorescence identification results of ultrasound microbubbles were analyzed,and the results of human ovarian cancer transplantation were counted,and the common and targeted contrast-enhanced ultrasound parameters(peak intensity,peak time and average transit time)at different time points after modeling were compared.At different time points,the nude mice were killed and the tumor was dissected.Levels of angiogenic molecules VEGFR1,Tie-2,cell proliferation proteins(Cyclin D1 and ELF5),cell invasion proteins(IGFBP2 and E-cadherin)were measured by using enzyme-linked immunosorbent assays.The correlation between targeted contrast-enhanced ultrasound parameters and cancer cell growth was analyzed.Results:There was no difference in morphology and size between targeted microvesicles and ordinary microvesicles.The targeted contrast microbubbles showed bright red fluorescence under fluorescence microscope,while the ordinary contrast microbubbles showed no fluorescence.At different time points after modeling,there were no significant differences in peak intensity,peak time and average transit time of ordinary contrast-enhanced ultrasound(all P>0.05),while there were significant differences in peak intensity,peak time and average transit time of targeted contrast-enhanced ultrasound(all P<0.05).At the same time point,the peak intensity and average transit time of targeted contrast-enhanced ultrasound were higher than those of ordinary contrast-enhanced ultrasound,and the peak time was lower than that of ordinary contrast-enhanced ultrasound(all P<0.05).The relative expressions of VEGFR1,Tie-2,Cyclin D1,ELF5,IGFBP2 and E-cadherin were significantly different at different time points after modeling(all P<0.05).Spearman correlation analysis showed that peak intensity and average transit time of targeted contrast-enhanced ultrasound were positively correlated with VEGFR1,Tie-2,Cyclin D1,IGFBP2 and E-cadherin levels,and negatively correlated with ELF5(all P<0.05); peak time was negatively correlated with VEGFR1,Tie-2,Cyclin D1,IGFBP2 and E-cadherin levels,and positively correlated with ELF5(all P<0.05).Conclusion:Targeted contrast-enhanced ultrasound imaging at different stages is superior to ordinary contrast-enhanced ultrasound.Contrast-enhanced ultrasound parameters have certain correlation with cancer cell growth,which is helpful for early diagnosis of ovarian cancer.

参考文献/References:

[1] 韩婷婷,任 艳,刘 明.经阴道彩色多普勒超声检查卵巢癌患者血流指标与肿瘤恶性度和血管新生的关系[J].中国肿瘤临床与康复,2021,28(6):699-703.
[2] 耿海慧,曹爱华.卵巢癌患者多种血清肿瘤标志物表达水平及其对疾病的诊断价值研究[J].陕西医学杂志,2022,51(7):886-889.
[3] 史阿娉,史成兴,杨志军,等.超声造影与彩色多普勒超声检查诊断卵巢肿瘤对比研究[J].陕西医学杂志,2019,48(5):628-631.
[4] 李月兴,胡秋根,黄伟俊,等.声触诊组织定量成像联合声诺维超声造影对乳腺BI-RADS 4类结节的鉴别诊断价值[J].现代生物医学进展,2021,21(13):2484-2488.
[5] 张 敏,张红河,来茂德.肿瘤免疫编辑中肿瘤微环境的变化[J].中华病理学杂志,2021,50(9):1098-1102.
[6] 刘少璇,李 佳,刘芳媛,等.基于“亢害承制”理论辨析卵巢癌因机证治[J].陕西中医,2022,43(7):925-928.
[7] 胡天祺,杨梦琪,杨 红,等.益气养阴方对卵巢癌术后患者化疗不良反应及生存质量的影响[J].陕西中医,2021,42(4):434-437.
[8] 李 玲,胡 刚,谭 亮,等.多模态无创诊断技术在卵巢癌早期诊断中的价值研究[J].中国医学装备,2021,18(8):90-93.
[9] 高宇峰,王 元.非编码RNAs在卵巢癌早期诊断中的研究进展[J].疑难病杂志,2022,21(6):665-668.
[10] 芦翼飞,孙君拓,剧 龙.血清HE4、CEA、CA125与卵巢癌早期诊断及疗效的关系[J].实用癌症杂志,2021,36(12):2044-2047.
[11] 杜成许,杨 坡,王超华,等.多普勒超声联合DKK-4、YKL-40检测对上皮性卵巢癌早期诊断价值[J].中国计划生育学杂志,2022,30(2):462-465.
[12] 邹媛媛,陶馨馨.卵巢子宫内膜异位症与卵巢癌的超声鉴别诊断[J].中国妇幼保健,2017,32(14):3362-3364.
[13] 李秀明.转移性卵巢癌的超声表现与病理对照分析[J].广西医学,2010,32(11):1406-1407.
[14] 范海燕,刘伟珂,董 敏,等.年龄差异对卵巢癌患者超声影像学特征及病理类型的影响[J].医学影像学杂志,2016,26(11):2132-2134,2135.
[15] 肖艳菊,郑红雨,杜阳春,等.二维超声及声脉冲辐射力弹性成像技术在卵巢癌术前分期中应用价值[J].中华实用诊断与治疗杂志,2021,35(8):848-850.
[16] 胡毓婷,黄晓民,缪 恺,等.超声造影联合血清学在卵巢癌早期筛查中的应用研究[J].中国超声医学杂志,2022,38(1):95-98.
[17] 顾文莉,张 园,田舒桐,等.超声造影定量参数对卵巢癌患者淋巴结转移的评价价值[J].中国妇幼保健,2020,35(1):172-174.
[18] 郭微群,朱长玉,周华玲,等.阴道超声造影联合CA125+HE4提高卵巢癌早期诊断的临床价值[J].浙江创伤外科,2021,26(6):1089-1091.
[19] Dai Y,Zhang X,Lv J,et al.Familial uterine leiomyoma-associated germline oncogenic mutant VEGFR1 is sensitive to mTOR inhibitor[J].Science Bulletin,2022,67(6):585-587.
[20] 孟 沙,秦恩杰,熊敏涵,等.eIF4E和cyclin D1在肿瘤中的调控机制及临床治疗进展[J].中国医药生物技术,2020,15(2):216-219,198.

备注/Memo

备注/Memo:
基金项目:陕西省社会发展科技攻关项目(2016SF-098)
更新日期/Last Update: 2022-12-05