[1]李 泽,单 伟,姜 东.Maresin1对糖尿病大鼠肾脏损伤的保护作用及机制研究[J].陕西医学杂志,2022,51(12):1467-1471.[doi:DOI:10.3969/j.issn.1000-7377.2022.12.001]
　LI Ze,SHAN Wei,JIANG Dong.Protective effect and mechanism of Maresin1 on renal injury in diabetic rats[J].,2022,51(12):1467-1471.[doi:DOI:10.3969/j.issn.1000-7377.2022.12.001]
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Maresin1对糖尿病大鼠肾脏损伤的保护作用及机制研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年12期
页码:
1467-1471
栏目:
基础研究
出版日期:
2022-12-05

文章信息/Info

Title:
Protective effect and mechanism of Maresin1 on renal injury in diabetic rats
作者:
李 泽; 单 伟; 姜 东
(锦州医科大学基础医学院解剖学教研室,辽宁 锦州 121001)
Author(s):
LI Ze; SHAN Wei; JIANG Dong
(Department of Anatomy,Basic Medical College,Jinzhou Medical University,Jinzhou 121001,China)
关键词:
糖尿病肾病;  Maresin1;  核因子-κB;  信号转导和转录激活因子3;  炎症因子;  链脲佐菌素
Keywords:
Diabetes nephropathy;  Maresin1;  NF-κB;  STAT3;  Inflammation factor;  Streptozotocin
分类号:
R 587.2
DOI:
DOI:10.3969/j.issn.1000-7377.2022.12.001
文献标志码:
A
摘要:
目的:探讨Maresin1对糖尿病大鼠肾脏损伤的保护作用及机制。方法:将SD大鼠分为四组,每组10只。对照组(Control组)不做任何处理; 糖尿病组(DM组)为单纯模型组; Maresin1预处理组(DM+Maresin1组)在成模8周后进行4 ng/g Maresin1腹腔注射给药,每周2次; DM+Maresin1+colivelin组在DM+Maresin1组基础上给予信号转导和转录激活因子3(STAT3)激活剂colivelin 2 mg/(kg·d)腹腔注射。采用单次腹腔注射链脲佐菌素55 mg/kg制备糖尿病模型。12周后,采用全自动生化分析仪检测血糖、血肌酐(Scr)和血尿素氮(BUN)水平。采用酶联免疫吸附试验检测血清炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)和IL-6水平。取肾脏组织进行HE染色、Masson染色和PAS染色。采用Western blot检测核因子-κB(NF-κB)/STAT3通路相关蛋白表达水平。结果:与Control组相比,DM组血糖、Scr、BUN、TNF-α、IL-1β、IL-6、NF-κB p65及p-STAT3水平明显增加(均P<0.05),肾脏病理损伤及纤维化严重。与DM组相比,DM+Maresin1组Scr、BUN、TNF-α、IL-1β、IL-6、NF-κB p65及p-STAT3水平明显下降(均P<0.05),肾脏病理损伤及纤维化减轻。经colivelin处理后,Maresin1对糖尿病大鼠肾脏损伤的保护作用出现部分逆转(均P<0.05)。结论:Maresin1对糖尿病大鼠肾脏损伤具有保护作用,其机制与抑制NF-κB/STAT3信号通路有关。
Abstract:
Objective:To investigate the protective effect and mechanism of Maresin1 on renal injury in diabetes rats.Methods:SD rats were divided into four groups with 10 rats in each group.The control group(Control group)did not receive any treatment; the diabetic group(DM group)was the model group; the Maresin1 pretreatment group(DM+Maresin1 group)was given 4 ng/g Maresin1 intraperitoneally twice a week after 8 weeks of modeling; the DM+Maresin1+colivelin group was given STAT3 activator colivelin 2 mg/(kg·d)intraperitoneally on the basis of DM+Maresin1 group.A single intraperitoneal injection of streptozotocin 55 mg/kg was used to establish a diabetic model.After 12 weeks,blood glucose,Scr and BUN levels were measured with a fully automated biochemical analyzer.Serum levels of inflammatory TNF-α,IL-1β and IL-6 were measured by enzyme-linked immunosorbent assay.Kidney tissues were stained with HE,Masson and PAS.Western blot was used to detect NF-κB/STAT3 pathway-related protein expression levels.Results:Compared with Control group,the levels of blood glucose,Scr,BUN,TNF-α,IL-1β,IL-6,NF-κB p65 and p-STAT3 in the DM group were significantly increased(all P<0.05),and the renal pathological damage and fibrosis were serious.Compared with the DM group,the levels of Scr,BUN,TNF-α,IL-1β,IL-6,NF-κB p65 and p-STAT3 in the DM+Maresin1 group were significantly decreased(all P<0.05),and the renal pathological damage and fibrosis were alleviated.After treatment with colivelin,the protective effect of Maresin1 on renal injury in diabetic rats was partially reversed(all P<0.05).Conclusion:Maresin1 has a protective effect on renal injury in diabetes rats,and its mechanism is related to the inhibition of NF-κB/STAT3 signaling pathway.

参考文献/References:

[1] Sagoo MK,Gnudi L.Diabetic nephropathy:An overview[J].Methods Mol Biol,2020,2067:3-7.
[2] 刘 芳,张立祥,王慧敏,等.养生益肾汤对糖尿病肾病患者血清细胞因子水平及肾功能的影响[J].陕西中医,2022,43(4):476-478,486.
[3] 孙 越,刘 阳.糖尿病肾病发病机制及中医药治疗研究进展[J].亚太传统医药,2021,17(5):207-212.
[4] 门 凌,何日明,杨曙东,等.p38 MAPK介导炎症在糖尿病肾病中作用研究进展[J].陕西医学杂志,2017,46(7):983-985.
[5] Zheng S,Ma M,Li Z,et al.Posttreatment of Maresin1 inhibits NLRP3 inflammasome activation via promotion of NLRP3 ubiquitination[J].FASEB J,2020,34(9):11944-11956.
[6] Xia H,Wang F,Wang M,et al.Maresin1 ameliorates acute lung injury induced by sepsis through regulating Th17/Treg balance[J].Life Sci,2020,254:117773.
[7] Morita Y,Kurano M,Sakai E,et al.Simultaneous analyses of urinary eicosanoids and related mediators identified tetranor-prostaglandin E metabolite as a novel biomarker of diabetic nephropathy[J].J Lipid Res,2021,62:100120.
[8] Wang F,Wang M,Wang J,et al.Maresin1 ameliorates sepsis-associated lung injury by inhibiting the activation of the JAK2/STAT3 and MAPK/NF-κB signaling pathways[J].Microb Pathog,2020,148:104468.
[9] Soto G,Rodríguez MJ,Fuentealba R,et al.Maresin 1,a proresolving lipid mediator,ameliorates liver ischemia-reperfusion injury and stimulates hepatocyte proliferation in sprague-dawley rats[J].Int J Mol Sci,2020,21(2):540.
[10] Cao Z,Huang D,Tang C,et al.Pyroptosis in diabetes and diabetic nephropathy[J].Clin Chim Acta,2022,531:188-196.
[11] 杨 雄,高 伟,邱万胜,等.白蛋白球蛋白比值对慢性肾脏病患者预后判断的价值研究[J].陕西医学杂志,2020,49(6):728-731.
[12] Saito-Sasaki N,Sawada Y,Nakamura M.Maresin-1 and inflammatory disease[J].Int J Mol Sci,2022,23(3):1367.
[13] Albuquerque-Souza E,Schulte F,Chen T,et al.Maresin-1 and resolvin E1 promote regenerative properties of periodontal ligament stem cells under inflammatory conditions[J].Front Immunol,2020,11:585530.
[14] Rodríguez MJ,Sabaj M,Tolosa G,et al.Maresin-1 prevents liver fibrosis by targeting Nrf2 and NF-κB,reducing oxidative stress and inflammation[J].Cells,2021,10(12):3406.
[15] Sun HJ,Xiong SP,Cao X,et al.Polysulfide-mediated sulfhydration of SIRT1 prevents diabetic nephropathy by suppressing phosphorylation and acetylation of p65 NF-κB and STAT3[J].Redox Biol,2021,38:101813.
[16] Ke G,Chen X,Liao R,et al.Receptor activator of NF-κB mediates podocyte injury in diabetic nephropathy[J].Kidney Int,2021,100(2):377-390.
[17] Li T,Zheng J,Wang Z,et al.Maresin 1 improves cognitive decline and ameliorates inflammation and blood-brain barrier damage in rats with chronic cerebral hypoperfusion[J].Brain Res,2022,1788:147936.
[18] Scalise V,Sanguinetti C,Neri T,et al.PCSK9 induces tissue factor expression by activation of TLR4/NF-κB signaling[J].Int J Mol Sci,2021,22(23):12640.
[19] 郭 黎,王 静.核因子-κB信号通路对肺炎模型小鼠免疫功能和炎症反应的影响[J].陕西医学杂志,2020,49(11):1383-1386.
[20] Butturini E,Carcereri PA,Mariotto S.Redox regulation of STAT1 and STAT3 signaling[J].Int J Mol Sci,2020,21(19):7034.
[21] Miao X,Xiang Y,Mao W,et al.TRIM27 promotes IL-6-induced proliferation and inflammation factor production by activating STAT3 signaling in HaCaT cells[J].Am J Physiol Cell Physiol,2020,318(2):272-281.

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备注/Memo

备注/Memo:
基金项目:辽宁省教育厅科学研究经费资助项目(JYTJCZR2020087)
更新日期/Last Update: 2022-12-05